S adenosylhomocysteine hydrolase (AHCY) (NM_000687) Human Untagged Clone

CAT#: SC319344

AHCY (untagged)-Human adenosylhomocysteinase (AHCY), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AHCY
• Synonyms: adoHcyase; SAHH
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000687.1 GCTGCCCGCCCAGTTCCTGTTCCCAGACTGAGGCCCAGCCCCCTTCGCCCGTTTCCATCA
  CGAGTGCCGCCAGCATGTCTGACAAACTGCCCTACAAAGTCGCCGACATCGGCCTGGCTG
  CCTGGGGACGCAAGGCCCTGGACATTGCTGAGAACGAGATGCCGGGCCTGATGCGTATGC
  GGGAGCGGTACTCGGCCTCCAAGCCACTGAAGGGCGCCCGCATCGCTGGCTGCCTGCACA
  TGACCGTGGAGACGGCCGTCCTCATTGAGACCCTCGTCACCCTGGGTGCTGAGGTGCAGT
  GGTCCAGCTGCAACATCTTCTCCACCCAGGACCATGCGGCGGCTGCCATTGCCAAGGCTG
  GCATTCCGGTGTATGCCTGGAAGGGCGAAACGGACGAGGAGTACCTGTGGTGCATTGAGC
  AGACCCTGTACTTCAAGGACGGGCCCCTCAACATGATTCTGGACGACGGGGGCGACCTCA
  CCAACCTCATCCACACCAAGTACCCGCAGCTTCTGCCAGGCATCCGAGGCATCTCTGAGG
  AGACCACGACTGGGGTCCACAACCTCTACAAGATGATGGCCAATGGGATCCTCAAGGTGC
  CTGCCATCAATGTCAATGACTCCGTCACCAAGAGCAAGTTTGACAACCTCTATGGCTGCC
  GGGAGTCCCTCATAGATGGCATCAAGCGGGCCACAGATGTGATGATTGCCGGCAAGGTAG
  CGGTGGTAGCAGGCTATGGTGATGTGGGCAAGGGCTGTGCCCAGGCCCTGCGGGGTTTCG
  GAGCCCGCGTCATCATCACCGAGATTGACCCCATCAACGCACTGCAGGCTGCCATGGAGG
  GCTATGAGGTGACCACCATGGATGAGGCCTGTCAGGAGGGCAACATCTTTGTCACCACCA
  CAGGCTGTATTGACATCATCCTTGGCCGGCACTTTGAGCAGATGAAGGATGATGCCATTG
  TGTGTAACATTGGACACTTTGACGTGGAGATCGATGTCAAGTGGCTCAACGAGAACGCCG
  TGGAGAAGGTGAACATCAAGCCGCAGGTGGACCGGTATCGGTTGAAGAATGGGCGCCGCA
  TCATCCTGCTGGCCGAGGGTCGGCTGGTCAACCTGGGTTGTGCCATGGGCCACCCCAGCT
  TCGTGATGAGTAACTCCTTCACCAACCAGGTGATGGCGCAGATCGAGCTGTGGACCCATC
  CAGACAAGTACCCCGTTGGGGTTCATTTCCTGCCCAAGAAGCTGGATGAGGCAGTGGCTG
  AAGCCCACCTGGGCAAGCTGAATGTGAAGTTGACCAAGCTAACTGAGAAGCAAGCCCAGT
  ACCTGGGCATGTCCTGTGATGGCCCCTTCAAGCCGGATCACTACCGCTACTGAGAGCCAG
  GTCTGCGTTTCACCCTCCAGCTGCTGTCCTTGCCCAGGCCCCACCTCTCCTCCCTAAGAG
  CTAATGGCACCAACTTTGTGATTGGTTTGTCAGTGTCCCCCATCGACTCTCTGGGGCTGA
  TCACTTAGTTTTTGGCCTCTGCTGCAGCCGTCATACTGTTCCAAATGTGGCAGCGGGAAC
  AGAGTACCCTCTTCAAGCCCCGGTCATGATGGAGGTCCCAGCCACAGGGAACCATGAGCT
  CAGTGGTCTTGGAACAGCTCACTAAGTCAGTCCTTCCTTAGCCTGGAAGTCAGTAGTGGA
  GTCACAAAGCCCATGTGTTTTGCCATCTAGGCCTTCACCTGGTCTGTGGACTTATACCTG
  TGTGCTTGGTTTACAGGTCCAGTGGTTCTTCAGCCCATGACAGATGAGAAGGGGCTATAT
  TGAAGGGCAAAGAGGAACTGTTGTTTGAATTTTCCTGAGAGCCTGGCTTAGTGCTGGGCC
  TTCTCTTAAACCTCATTACAATGAGGTTAGTACTTTTAGTCCCTGTTTTACAGGGGTTAG
  AATAGACTGTTAAGGGGCAACTGAGAAAGAACAGAGAAGTGACAGCTAGGGGTTGAGAGG
  GGCCAGAAAAACATGAATGCAGGCAGATTTCGTGAAATCTGCCACCACTTTATAACCAGA
  TGGTTCCTTTCTCAACCCTGGGTCAAAAAGAGAATAATTTGGCCTATAATGTTAAAAGAA
  AGCAGGAAGGTGGGTAAATAAAAATCTTGGTGCCTGGAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000687
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000687.1, NP_000678.1
• RefSeq Size: 2110 bp
• RefSeq ORF: 1299 bp
• Locus ID: 191
• Cytogenetics: 20q11.22
• Domains: AdoHcyase
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
• Gene Summary: 'S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]'
  Transcript Variant: This variant (1) encodes isoform 1.