PRP19 (PRPF19) (NM_014502) Human Tagged ORF Clone
CAT#: RC200810
PRPF19 (Myc-DDK-tagged)-Human PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) (PRPF19)
"NM_014502" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PRPF19 |
Synonyms | hPSO4; NMP200; PRP19; PSO4; SNEV; UBOX4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200810 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTAATCTGCTCCATCTCTAACGAAGTGCCGGAGCACCCATGTGTATCCCCTGTCTCTAATCATG TTTATGAGCGGCGGCTCATCGAGAAGTACATTGCGGAGAATGGTACCGACCCCATCAACAACCAGCCTCT CTCCGAGGAGCAGCTCATCGACATCAAAGTTGCTCACCCAATCCGGCCCAAGCCTCCCTCAGCCACCAGC ATCCCGGCCATTCTGAAAGCTTTGCAGGATGAGTGGGATGCAGTCATGCTGCACAGCTTCACTCTGCGCC AGCAGCTGCAGACAACCCGCCAAGAGCTGTCACACGCTCTGTACCAGCACGATGCCGCCTGCCGTGTCAT TGCCCGTCTCACCAAGGAAGTCACTGCTGCCCGAGAAGCTCTGGCTACCCTGAAACCACAGGCTGGCCTC ATTGTGCCCCAGGCTGTGCCAAGTTCCCAACCAAGTGTTGTGGGTGCGGGTGAGCCAATGGATTTGGGTG AGCTGGTGGGAATGACCCCAGAGATTATTCAGAAGCTTCAAGACAAAGCCACTGTGCTAACCACGGAGCG CAAGAAGAGAGGGAAGACTGTGCCTGAGGAGCTGGTGAAGCCAGAAGAGCTCAGCAAATACCGGCAGGTG GCATCCCACGTGGGGTTGCACAGTGCCAGCATTCCTGGGATCCTGGCCCTGGACCTCTGCCCGTCCGACA CCAACAAGATCCTCACTGGTGGGGCGGATAAAAATGTCGTTGTGTTTGACAAAAGTTCTGAACAAATCCT GGCTACCCTCAAAGGCCATACCAAGAAGGTCACCAGCGTGGTGTTTCACCCTTCCCAGGACCTGGTGTTT TCTGCTTCCCCCGATGCCACTATCAGGATTTGGTCGGTCCCCAATGCCTCTTGTGTACAGGTGGTTCGGG CCCATGAGAGTGCTGTGACAGGCCTCAGCCTTCATGCCACTGGCGACTATCTCCTGAGCTCCTCCGATGA TCAGTACTGGGCTTTCTCTGACATCCAGACAGGGCGTGTGCTCACCAAGGTGACAGATGAGACCTCCGGC TGCTCTCTCACCTGTGCACAGTTCCACCCTGACGGACTCATCTTTGGAACAGGAACCATGGACTCTCAGA TCAAGATCTGGGACTTGAAGGAACGTACTAATGTGGCCAACTTCCCTGGCCACTCGGGCCCCATCACTAG CATCGCCTTCTCTGAGAATGGTTACTACCTGGCTACAGCGGCTGATGACTCCTCTGTCAAGCTCTGGGAT CTGCGCAAGCTTAAGAACTTTAAGACTTTGCAGCTGGATAACAACTTTGAGGTAAAGTCACTGATCTTTG ACCAGAGTGGTACCTACCTGGCTCTTGGGGGCACGGATGTCCAGATCTACATCTGCAAACAATGGACGGA GATTCTTCACTTTACAGAGCATAGCGGCCTGACCACAGGGGTGGCCTTCGGGCATCACGCCAAGTTCATC GCTTCAACAGGCATGGACAGAAGCCTCAAGTTCTACAGCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200810 protein sequence
Red=Cloning site Green=Tags(s) MSLICSISNEVPEHPCVSPVSNHVYERRLIEKYIAENGTDPINNQPLSEEQLIDIKVAHPIRPKPPSATS IPAILKALQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALATLKPQAGL IVPQAVPSSQPSVVGAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQV ASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVF SASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSG CSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWD LRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFI ASTGMDRSLKFYSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014502 |
ORF Size | 1512 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014502.1, NM_014502.2, NM_014502.3, NM_014502.4, NP_055317.1 |
RefSeq Size | 2342 bp |
RefSeq ORF | 1515 bp |
Locus ID | 27339 |
Domains | WD40, U-box |
Protein Families | Druggable Genome |
Protein Pathways | Spliceosome, Ubiquitin mediated proteolysis |
MW | 55.2 kDa |
Gene Summary | PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]). [supplied by OMIM, Sep 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC319066 | PRPF19 (untagged)-Human PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) (PRPF19) |
USD 310.00 |
|
RG200810 | PRPF19 (GFP-tagged) - Human PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) (PRPF19) |
USD 460.00 |
|
RC200810L1 | Lenti ORF clone of Human PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) (PRPF19), Myc-DDK-tagged |
USD 768.00 |
|
RC200810L2 | Lenti ORF clone of Human PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) (PRPF19), mGFP tagged |
USD 620.00 |
|
RC200810L3 | Lenti ORF clone of Human PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) (PRPF19), Myc-DDK-tagged |
USD 768.00 |
|
RC200810L4 | Lenti ORF clone of Human PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) (PRPF19), mGFP tagged |
USD 768.00 |
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