ZNF426 (NM_024106) Human Tagged ORF Clone

CAT#: RC200852

ZNF426 (Myc-DDK-tagged)-Human zinc finger protein 426 (ZNF426)

Product Images

Western blot validation of overexpression lysate (Cat# LY411340) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200852 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF426
• Synonyms: K-RBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200852 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTGCTGATTTGTCCCATGGACATTATCTTTCTGGGGACCCAGTTTGCCTTCATGAAGAAAAGA
  CACCAGCAGGAAGAATAGTGGCTGACTGCCTAACAGATTGTTATCAGGATTCAGTGACCTTTGACGATGT
  GGCTGTGGACTTCACCCAGGAGGAGTGGACTTTACTGGACTCAACTCAGAGAAGCCTCTACAGTGACGTG
  ATGCTGGAGAACTACAAGAACCTGGCCACAGTAGGAGGTCAGATCATCAAACCCAGTCTAATCTCTTGGT
  TGGAACAAGAAGAGTCAAGGACAGTTCAGGGAGGAGTTCTCCAAGGATGGGAAATGCGACTTGAAACCCA
  GTGGTCTATACTTCAGCAGGACTTTTTGAGGGGTCAGACATCCATTGGGATACAATTGGAAGGAAAACAC
  AATGGAAGGGAACTCTGTGACTGTGAGCAATGTGGAGAAGTCTTCAGTGAACACTCATGCCTTAAGACGC
  ACGTGAGAACTCAAAGTACAGGGAACACTCATGACTGTAATCAGTATGGAAAAGATTTCCTTACCCTGTG
  TGAGAAAACCTCTACTGGTGAGAAACTTTCTGAGTTTAATCAGAGTGAAAAAATCTTCAGCCTGACACCA
  AATATTGTATACCAGAGAACTAGCACACAAGAAAAGTCATTTGAATGTAGTCACTGTGGAAAATCCTTCA
  TTAATGAGTCATACCTTCAGGCACATATGAGAACTCACAATGGAGAAAAACTCTACGAATGGAGGAATTA
  TGGGCCAGGTTTTATTGACTCTACAAGCCTTTCTGTGCTTATAGAAACCCTCAATGCAAAAAAGCCCTAC
  AAATGTAAGGAATGTGGAAAAGGCTATAGATACCCAGCCTACCTCAGTATTCACATGCGAACCCACACTG
  GGGAGAAACCATATGAATGTAAGGAATGTGGGAAAGCCTTCAATTATTCCAACTCATTTCAGATACATGG
  AAGAACTCACACTGGAGAGAAACCCTATGTATGTAAGGAATGTGGGAAAGCCTTCACTCAGTACTCGGGC
  CTTAGTATGCATGTACGATCTCACAGTGGAGACAAGCCCTATGAATGTAAGGAATGTGGGAAATCCTTCC
  TTACATCCTCACGCCTTATTCAACATATAAGAACTCACACTGGAGAGAAGCCTTTTGTATGTGTTGAATG
  TGGGAAAGCCTTTGCAGTTTCCTCAAATCTTAGTGGACATTTGAGAACTCACACTGAAGAGAAGGCCTGT
  GAGTGTAAGATATGTGGGAAAGTATTTGGGTATCCCTCATGTCTTAATAATCACATGCGAACGCACAGTG
  CCCAGAAACCATACACCTGTAAGGAATGTGGGAAGGCTTTTAACTATTCCACCCACCTTAAAATTCACAT
  GCGAATCCACACTGGAGAAAAACCCTATGAGTGTAAACAATGTGGAAAGGCCTTCAGTCATTCCAGTTCA
  TTTCAAATACATGAAAGGACTCACACTGGAGAGAAACCCTATGAATGCAAGGAGTGTGGGAAAGCCTTCA
  CGTGTTCCAGTTCCTTTAGAATTCATGAAAAAACTCACACAGAAGAGAAACCCTATAAATGTCAGCAATG
  CGGGAAAGCTTACAGTCATCCCCGTTCACTTCGAAGACATGAACAAATTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200852 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAADLSHGHYLSGDPVCLHEEKTPAGRIVADCLTDCYQDSVTFDDVAVDFTQEEWTLLDSTQRSLYSDV
  MLENYKNLATVGGQIIKPSLISWLEQEESRTVQGGVLQGWEMRLETQWSILQQDFLRGQTSIGIQLEGKH
  NGRELCDCEQCGEVFSEHSCLKTHVRTQSTGNTHDCNQYGKDFLTLCEKTSTGEKLSEFNQSEKIFSLTP
  NIVYQRTSTQEKSFECSHCGKSFINESYLQAHMRTHNGEKLYEWRNYGPGFIDSTSLSVLIETLNAKKPY
  KCKECGKGYRYPAYLSIHMRTHTGEKPYECKECGKAFNYSNSFQIHGRTHTGEKPYVCKECGKAFTQYSG
  LSMHVRSHSGDKPYECKECGKSFLTSSRLIQHIRTHTGEKPFVCVECGKAFAVSSNLSGHLRTHTEEKAC
  ECKICGKVFGYPSCLNNHMRTHSAQKPYTCKECGKAFNYSTHLKIHMRIHTGEKPYECKQCGKAFSHSSS
  FQIHERTHTGEKPYECKECGKAFTCSSSFRIHEKTHTEEKPYKCQQCGKAYSHPRSLRRHEQIH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024106
• ORF Size: 1662 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024106.1, NM_024106.2, NP_077011.1
• RefSeq Size: 2320 bp
• RefSeq ORF: 1665 bp
• Locus ID: 79088
• Domains: KRAB, zf-BED, zf-C2H2
• Protein Families: Transcription Factors
• MW: 63.1 kDa
• Gene Summary: Kaposi's sarcoma-associated herpesvirus (KSHV) can be reactivated from latency by the viral protein RTA. The protein encoded by this gene is a zinc finger transcriptional repressor that interacts with RTA to modulate RTA-mediated reactivation of KSHV. While the encoded protein can repress KSHV reactivation, RTA can induce degradation of this protein through the ubiquitin-proteasome pathway to overcome the repression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]