Vgl4 (VGLL4) (NM_014667) Human Tagged ORF Clone

CAT#: RC200886

VGLL4 (Myc-DDK-tagged)-Human vestigial like 4 (Drosophila) (VGLL4), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY415121) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200886 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified VGLL4 protein (Cat# TP300886). The protein was produced from HEK293T cells transfected with VGLL4 cDNA clone (Cat# RC200886) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VGLL4
• Synonyms: VGL-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200886 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGCCATTGGATGTTTTGTCCAGGGCAGCATCTCTGGTGCATGCTGATGACGAAAAACGCGAAG
  CTGCTCTCAGGGGAGAACCCAGAATGCAGACCCTGCCGGTGGCCTCTGCCCTCAGCAGTCACCGCACCGG
  CCCTCCCCCAATCAGCCCCAGCAAGAGGAAGTTCAGCATGGAGCCAGGTGACGAGGACCTAGACTGTGAC
  AACGACCACGTCTCCAAAATGAGTCGCATCTTCAACCCCCATCTGAACAAGACTGCCAATGGAGACTGCC
  GCAGAGACCCCCGGGAGCGGAGCCGCAGCCCCATCGAGCGCGCTGTGGCCCCCACCATGAGCCTGCACGG
  CAGCCACCTGTACACCTCCCTCCCCAGCCTTGGCCTGGAGCAGCCCCTCGCACTGACCAAGAACAGCCTG
  GACGCCAGCAGGCCAGCCGGCCTCTCGCCCACACTGACCCCGGGGGAGCGGCAGCAGAACCGGCCCTCCG
  TGATCACCTGTGCCTCGGCTGGCGCCCGCAACTGCAACCTCTCGCACTGCCCCATCGCGCACAGCGGCTG
  TGCCGCGCCCGGGCCTGCCAGCTACCGGAGGCCACCGAGCGCTGCCACCACCTGTGACCCCGTGGTGGAG
  GAGCATTTCCGCAGGAGCCTGGGCAAGAATTACAAGGAGCCCGAGCCGGCACCCAACTCCGTGTCCATCA
  CGGGCTCCGTGGACGACCACTTTGCCAAAGCTCTGGGTGACACGTGGCTCCAGATCAAAGCGGCCAAGGA
  CGGAGCATCCAGCAGCCCTGAGTCCGCCTCTCGCAGGGGCCAGCCCGCCAGCCCCTCTGCCCACATGGTC
  AGCCACAGTCACTCCCCCTCTGTGGTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200886 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METPLDVLSRAASLVHADDEKREAALRGEPRMQTLPVASALSSHRTGPPPISPSKRKFSMEPGDEDLDCD
  NDHVSKMSRIFNPHLNKTANGDCRRDPRERSRSPIERAVAPTMSLHGSHLYTSLPSLGLEQPLALTKNSL
  DASRPAGLSPTLTPGERQQNRPSVITCASAGARNCNLSHCPIAHSGCAAPGPASYRRPPSAATTCDPVVE
  EHFRRSLGKNYKEPEPAPNSVSITGSVDDHFAKALGDTWLQIKAAKDGASSSPESASRRGQPASPSAHMV
  SHSHSPSVVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014667
• ORF Size: 870 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014667.1, NM_014667.2, NM_014667.3, NP_055482.1
• RefSeq Size: 3756 bp
• RefSeq ORF: 873 bp
• Locus ID: 9686
• Protein Families: Transcription Factors
• MW: 30.9 kDa