SNX11 (NM_152244) Human Tagged ORF Clone

CAT#: RC200906

SNX11 (Myc-DDK-tagged)-Human sorting nexin 11 (SNX11), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY407675) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200906 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SNX11 protein (Cat# TP300906). The protein was produced from HEK293T cells transfected with SNX11 cDNA clone (Cat# RC200906) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNX11
• Synonyms: MGC111019
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200906 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTTTGGTGTAGGATGTCGGAGAACCAAGAACAGGAGGAGGTGATTACAGTGCGTGTTCAGGACC
  CCCGAGTGCAGAATGAGGGCTCCTGGAACTCTTATGTGGATTATAAGATATTCCTCCATACCAACAGCAA
  AGCCTTTACTGCCAAGACTTCCTGTGTGCGGCGCCGCTACCGTGAGTTCGTGTGGCTGAGAAAGCAGCTA
  CAGAGAAATGCTGGTTTGGTGCCTGTTCCTGAACTTCCTGGGAAGTCAACCTTCTTCGGCACCTCAGATG
  AGTTCATTGAGAAGCGACGACAAGGTCTGCAGCACTTCCTTGAAAAGGTCCTGCAGAGTGTGGTTCTCCT
  GTCAGACAGCCAGTTGCACCTATTCCTGCAAAGCCAGCTCTCGGTGCCTGAGATAGAAGCCTGTGTCCAG
  GGCCGAAGTACCATGACTGTGTCTGATGCCATTCTTCGATATGCTATGTCAAACTGTGGCTGGGCCCAGG
  AAGAGAGGCAGAGCTCTTCTCACCTGGCTAAAGGAGACCAGCCTAAGAGTTGCTGCTTTCTTCCAAGATC
  GGGTAGGAGGAGCTCTCCCTCACCGCCTCCCAGTGAAGAAAAGGACCATTTAGAAGTGTGGGCTCCAGTT
  GTTGACTCTGAGGTTCCTTCCTTGGAAAGTCCCACTCTCCCACCCCTCTCCTCACCATTATGCTGTGATT
  TTGGAAGACCCAAAGAGGGAACCTCCACTCTTCAGTCTGTGAGGAGGGCTGTGGGAGGAGATCATGCTGT
  GCCTTTGGACCCTGGTCAGTTAGAAACAGTTTTGGAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200906 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGFWCRMSENQEQEEVITVRVQDPRVQNEGSWNSYVDYKIFLHTNSKAFTAKTSCVRRRYREFVWLRKQL
  QRNAGLVPVPELPGKSTFFGTSDEFIEKRRQGLQHFLEKVLQSVVLLSDSQLHLFLQSQLSVPEIEACVQ
  GRSTMTVSDAILRYAMSNCGWAQEERQSSSHLAKGDQPKSCCFLPRSGRRSSPSPPPSEEKDHLEVWAPV
  VDSEVPSLESPTLPPLSSPLCCDFGRPKEGTSTLQSVRRAVGGDHAVPLDPGQLETVLEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152244
• ORF Size: 810 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152244.1, NP_689450.1
• RefSeq Size: 2402 bp
• RefSeq ORF: 813 bp
• Locus ID: 29916
• Domains: PX
• MW: 30.4 kDa
• Gene Summary: This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members. This gene encodes a protein of unknown function. This gene results in two transcript variants differing in the 5' UTR, but encoding the same protein. [provided by RefSeq, Jul 2008]