BEGAIN (NM_020836) Human Tagged ORF Clone

CAT#: RC200943

BEGAIN (Myc-DDK-tagged)-Human brain-enriched guanylate kinase-associated homolog (rat) (BEGAIN), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY431998) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228971 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified BEGAIN protein (Cat# TP300943). The protein was produced from HEK293T cells transfected with BEGAIN cDNA clone (Cat# RC200943) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BEGAIN
• Synonyms: KIAA1446
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200943 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAACTCAGCGCGCTGCAGGAGCAGAAGGGCGAGCTGCGCAAGCGGCTGTCCTACACCACACACA
  AGCTCGAGAAGCTCGAGACCGAGTTCGACTCCACGCGCCACTACCTGGAGATCGAGCTGCGGCGCGCGCA
  GGAGGAACTGGAGAAGGTCACGGAGAAGCTGCGCAGGATTCAGAGCAACTACATGGCACTGCAGAGGATC
  AACCAGGAGCTGGAGGACAAGCTGTACCGCATGGGCCAGCACTATGAGGAGGAGAAGCGTGCGCTGAGCC
  ACGAGATTGTTGCCCTCAACAGCCATCTGCTAGAAGCCAAGGTGACCATCGACAAGCTGTCAGAGGACAA
  CGAGCTCTATAGGAAGGACTGCAATCTAGCGGCCCAGCTGCTGCAGTGCAGCCAGACCTACGGCAGGGTC
  CACAAGGTGTCTGAGCTGCCCTCGGATTTCCAGGAGCGCGTGAGCCTGCACATGGAGAAGCACGGCTGCA
  GCCTGCCATCCCCGCTCTGCCACCCGGCCTACGCCGACAGCGTCCCCACCTGCGTCATTGCCAAGGTGCT
  GGAGAAGCCGGACCCCGCCAGCCTGTCCTCCCGCCTGTCCGATGCCTCCGCCCGCGACCTGGCCTTCTGC
  GACGGGGTGGAGAAACCAGGCCCGCGGCCCCCCTACAAGGGAGACATCTACTGCAGTGACACAGCCCTCT
  ACTGCCCGGAGGAGCGGCGGCGAGACCGGCGGCCTAGCGTGGACGCGCCCGTGACCGACGTGGGCTTCCT
  GCGGGCCCAGAACTCCACTGACAGCGCGGCCGAGGAGGAGGAGGAGGCCGAGGCGGCGGCCTTCCCGGCG
  GGCTTCCAGCATGAGGCCTTCCCCAGCTACGCAGGCTCACTGCCCACGTCCAGCTCCTACTCCAGCTTCA
  GCGCCACGTCGGAGGAGAAGGAGCACGCGCAGGCCAGCACGCTGACCGCGTCGCAGCAGGCCATCTACCT
  GAACAGCCGCGACGAGCTCTTCGACCGCAAGCCACCCGCCACCACCTACGAGGGCAGCCCTCGCTTTGCC
  AAGGCCACGGCCGCGGTGGCGGCCCCGCTGGAGGCCGAAGTGGCCCCAGGCTTCGGGCGGACCATGTCAC
  CGTACCCGGCCGAGACCTTCCGCTTCCCGGCCTCTCCGGGTCCCCAGCAGGCCCTGATGCCCCCAAACCT
  GTGGAGCCTGCGGGCCAAGCCGGGGACCGCCCGGCTCCCCGGGGAGGACATGAGGGGCCAGTGGCGTCCC
  CTGAGCGTGGAGGACATCGGCGCCTACTCCTACCCCGTGAGCGCTGCCGGCCGCGCCTCACCCTGCAGCT
  TCTCTGAACGCTACTACGGCGGGGCCGGGGGCAGCCCGGGCAAGAAGGCCGACGGCCGCGCCAGCCCGCT
  CTACGCCAGCTACAAGGCCGACAGCTTCTCCGAGGGGGACGACCTCTCCCAGGGCCACCTGGCAGAGCCC
  TGCTTCCTGCGGGCGGGCGGCGACCTGAGCCTCAGTCCCGGCCGCTCGGCTGACCCACTGCCCGGCTATG
  CACCCAGCGAGGGGGGGGACGGGGACAGGCTCGGGGTGCAGCTGTGTGGGACCGCCAGCAGCCCTGAGCC
  CGAGCAGGGTTCCAGGGACTCCTTGGAGCCGAGCTCCATGGAGGCCTCCCCGGAAATGCATCCTGCCGCC
  CGCCTCAGCCCCCAGCAGGCCTTTCCGCGGACTGGTGGCTCGGGGCTGAGCCGCAAGGACAGCCTCACCA
  AGGCCCAGCTCTACGGAACCTTGCTCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200943 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKLSALQEQKGELRKRLSYTTHKLEKLETEFDSTRHYLEIELRRAQEELEKVTEKLRRIQSNYMALQRI
  NQELEDKLYRMGQHYEEEKRALSHEIVALNSHLLEAKVTIDKLSEDNELYRKDCNLAAQLLQCSQTYGRV
  HKVSELPSDFQERVSLHMEKHGCSLPSPLCHPAYADSVPTCVIAKVLEKPDPASLSSRLSDASARDLAFC
  DGVEKPGPRPPYKGDIYCSDTALYCPEERRRDRRPSVDAPVTDVGFLRAQNSTDSAAEEEEEAEAAAFPA
  GFQHEAFPSYAGSLPTSSSYSSFSATSEEKEHAQASTLTASQQAIYLNSRDELFDRKPPATTYEGSPRFA
  KATAAVAAPLEAEVAPGFGRTMSPYPAETFRFPASPGPQQALMPPNLWSLRAKPGTARLPGEDMRGQWRP
  LSVEDIGAYSYPVSAAGRASPCSFSERYYGGAGGSPGKKADGRASPLYASYKADSFSEGDDLSQGHLAEP
  CFLRAGGDLSLSPGRSADPLPGYAPSEGGDGDRLGVQLCGTASSPEPEQGSRDSLEPSSMEASPEMHPAA
  RLSPQQAFPRTGGSGLSRKDSLTKAQLYGTLLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020836
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020836.1, NM_020836.2, NM_020836.3, NP_065887.1
• RefSeq Size: 2689 bp
• RefSeq ORF: 1782 bp
• Locus ID: 57596
• Protein Families: Druggable Genome
• MW: 64.8 kDa
• Gene Summary: May sustain the structure of the postsynaptic density (PSD). [UniProtKB/Swiss-Prot Function]