ST6GALNAC5 (NM_030965) Human Tagged ORF Clone

CAT#: RC200989

ST6GALNAC5 (Myc-DDK-tagged)-Human ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 (ST6GALNAC5)

Product Images

Western blot validation of overexpression lysate (Cat# LY410629) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200989 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ST6GALNAC5
• Synonyms: SIAT7-E; SIAT7E; ST6GalNAcV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200989 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACCCTGATGCGCCATGGTCTGGCAGTGTGTTTAGCGCTCACCACCATGTGCACCAGCTTGTTGC
  TAGTGTACAGCAGCCTCGGCGGCCAGAAGGAGCGGCCCCCGCAGCAGCAGCAGCAGCAGCAGCAACAGCA
  GCAGCAGGCGTCGGCCACCGGCAGCTCGCAGCCGGCGGCGGAGAGCAGCACCCAGCAGCGCCCCGGGGTC
  CCCGCGGGACCGCGGCCACTGGACGGATACCTCGGAGTGGCGGACCACAAGCCCCTGAAAATGCACTGCA
  GGGACTGTGCCCTGGTGACCAGCTCAGGGCATCTGCTGCACAGTCGGCAAGGCTCCCAGATTGACCAGAC
  AGAGTGTGTCATCCGCATGAATGACGCCCCCACACGCGGCTATGGGCGTGACGTGGGCAATCGCACCAGC
  CTGAGGGTCATCGCGCATTCCAGCATCCAGAGGATCCTCCGCAACCGCCATGACCTGCTCAACGTGAGCC
  AGGGCACCGTGTTCATCTTCTGGGGCCCCAGCAGCTACATGCGGCGGGACGGCAAGGGCCAGGTCTACAA
  CAACCTGCATCTCCTGAGCCAGGTGCTGCCCCGGCTGAAGGCCTTCATGATTACTCGCCACAAGATGCTG
  CAGTTTGATGAGCTCTTCAAGCAGGAGACTGGCAAAGACAGGAAGATATCCAACACTTGGCTCAGCACTG
  GCTGGTTTACAATGACAATTGCACTGGAGCTCTGTGACAGGATCAATGTTTATGGCATGGTGCCCCCAGA
  CTTCTGCAGGGATCCCAATCACCCTTCAGTACCTTATCATTATTATGAACCTTTTGGACCTGATGAATGT
  ACAATGTACCTCTCCCATGAGCGAGGACGCAAGGGCAGTCATCACCGCTTTATCACAGAGAAACGAGTCT
  TTAAGAACTGGGCACGGACATTCAATATTCACTTTTTTCAACCAGACTGGAAACCAGAATCACTTGCTAT
  AAATCATCCTGAGAATAAACCTGTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200989 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKTLMRHGLAVCLALTTMCTSLLLVYSSLGGQKERPPQQQQQQQQQQQQASATGSSQPAAESSTQQRPGV
  PAGPRPLDGYLGVADHKPLKMHCRDCALVTSSGHLLHSRQGSQIDQTECVIRMNDAPTRGYGRDVGNRTS
  LRVIAHSSIQRILRNRHDLLNVSQGTVFIFWGPSSYMRRDGKGQVYNNLHLLSQVLPRLKAFMITRHKML
  QFDELFKQETGKDRKISNTWLSTGWFTMTIALELCDRINVYGMVPPDFCRDPNHPSVPYHYYEPFGPDEC
  TMYLSHERGRKGSHHRFITEKRVFKNWARTFNIHFFQPDWKPESLAINHPENKPVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030965
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030965.1, NM_030965.2, NP_112227.1
• RefSeq Size: 2048 bp
• RefSeq ORF: 1011 bp
• Locus ID: 81849
• Domains: Glyco_transf_29
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
• MW: 38.4 kDa
• Gene Summary: The protein encoded by this gene is a Golgi type II transmembrane glycosyltransferase. The encoded protein catalyzes the transfer of sialic acid to cell surface proteins to modulate cell-cell interactions. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]