EXOSC4 (NM_019037) Human Tagged ORF Clone

CAT#: RC201058

EXOSC4 (Myc-DDK-tagged)-Human exosome component 4 (EXOSC4)

Product Images

Western blot validation of overexpression lysate (Cat# LY412805) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201058 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXOSC4
• Synonyms: hRrp41p; p12A; RRP41; RRP41A; Rrp41p; SKI6; Ski6p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201058 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGCTGGAGCTCTTGTCGGACCAGGGCTACCGGGTGGACGGGCGGCGCGCCGGGGAGCTGCGCA
  AGATCCAGGCGCGGATGGGCGTGTTCGCGCAGGCTGACGGCTCGGCCTACATTGAGCAGGGCAACACCAA
  GGCACTGGCTGTGGTCTACGGCCCGCACGAGATCCGGGGCTCCCGGGCTCGAGCCCTGCCGGACAGGGCC
  CTAGTGAACTGTCAATATAGTTCAGCGACCTTCAGCACAGGTGAGCGCAAGCGACGGCCACATGGGGACC
  GTAAGTCCTGTGAGATGGGCCTGCAGCTCCGCCAGACTTTCGAAGCAGCCATCCTCACACAGCTGCACCC
  ACGCTCCCAGATTGATATCTATGTGCAGGTGCTACAGGCAGATGGTGGGACCTATGCAGCTTGTGTGAAT
  GCAGCCACGCTGGCAGTGCTGGATGCCGGGATACCCATGAGAGACTTTGTGTGTGCGTGCTCAGCTGGCT
  TCGTGGACGGCACAGCCCTGGCGGACCTCAGCCATGTGGAGGAAGCAGCTGGTGGCCCCCAGCTGGCCCT
  GGCCCTGCTGCCAGCCTCAGGACAGATTGCGCTGCTTGAGATGGATGCCCGGCTGCACGAGGACCACCTG
  GAGCGGGTGTTGGAGGCTGCTGCCCAGGCTGCCCGAGATGTGCACACCCTCTTAGATCGAGTGGTCCGGC
  AGCATGTGCGTGAGGCCTCTATCTTGCTGGGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201058 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGLELLSDQGYRVDGRRAGELRKIQARMGVFAQADGSAYIEQGNTKALAVVYGPHEIRGSRARALPDRA
  LVNCQYSSATFSTGERKRRPHGDRKSCEMGLQLRQTFEAAILTQLHPRSQIDIYVQVLQADGGTYAACVN
  AATLAVLDAGIPMRDFVCACSAGFVDGTALADLSHVEEAAGGPQLALALLPASGQIALLEMDARLHEDHL
  ERVLEAAAQAARDVHTLLDRVVRQHVREASILLGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019037
• ORF Size: 735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019037.1, NM_019037.2, NP_061910.1
• RefSeq Size: 948 bp
• RefSeq ORF: 738 bp
• Locus ID: 54512
• Domains: RNase_PH_C
• Protein Pathways: RNA degradation
• MW: 26.4 kDa