POLA2 (NM_002689) Human Tagged ORF Clone

CAT#: RC201145

POLA2 (Myc-DDK-tagged)-Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2)

Product Images

Western blot validation of overexpression lysate (Cat# LY419168) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201145 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POLA2
• Synonyms: FLJ21662; FLJ37250
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201145 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCATCCGCCCAGCAGCTGGCGGAGGAGCTGCAGATCTTCGGCCTAGACTGCGAGGAGGCTCTAA
  TTGAGAAATTGGTAGAGCTTTGTGTTCAGTATGGACAGAATGAGGAGGGAATGGTAGGCGAGCTTATAGC
  CTTCTGCACCAGCACACATAAAGTTGGCCTTACCTCAGAGATCCTGAACTCTTTTGAGCATGAGTTTCTG
  AGCAAAAGATTATCGAAAGCCAGGCATAGTACCTGCAAGGACAGTGGCCATGCAGGAGCTAGAGACATTG
  TTTCCATTCAAGAGCTAATTGAAGTGGAAGAAGAAGAGGAAATCCTCTTGAACTCTTACACCACACCTTC
  AAAGGGTTCTCAGAAGCGAGCTATCTCTACCCCAGAAACCCCCCTAACAAAAAGGAGTGTGTCAACTCGT
  AGCCCCCATCAGCTACTCTCACCGTCAAGTTTCTCTCCAAGTGCTACTCCCTCCCAGAAATACAACTCAC
  GAAGTAACCGAGGAGAAGTGGTTACCTCCTTCGGCTTAGCACAGGGAGTATCTTGGTCTGGGAGAGGAGG
  AGCTGGAAACATCAGCCTGAAGGTCTTGGGATGTCCAGAGGCACTAACTGGGAGCTACAAATCCATGTTT
  CAGAAGCTCCCAGACATTCGAGAAGTTCTGACCTGTAAGATAGAAGAACTTGGCAGCGAACTCAAGGAAC
  ATTACAAGATTGAAGCTTTCACTCCTTTGCTAGCCCCAGCACAGGAGCCTGTCACTCTGCTGGGCCAGAT
  TGGCTGTGATAGCAACGGGAAGCTGAACAACAAGTCAGTGATTCTCGAGGGAGACCGGGAACATTCCTCG
  GGTGCTCAAATTCCAGTGGATTTATCTGAGCTTAAGGAATATTCTCTGTTTCCTGGACAGGTTGTAATTA
  TGGAAGGAATCAACACCACTGGTAGGAAACTTGTTGCCACCAAACTCTACGAGGGTGTGCCACTTCCATT
  TTATCAGCCCACTGAAGAGGATGCAGACTTTGAGCAAAGCATGGTCCTGGTTGCCTGTGGACCATACACC
  ACATCTGACAGCATCACGTATGACCCCCTGCTTGACCTGATTGCTGTCATCAACCATGACCGGCCAGATG
  TCTGCATCCTGTTTGGCCCTTTCCTGGATGCTAAGCATGAACAGGTGGAGAATTGTCTACTGACAAGTCC
  ATTTGAAGACATTTTCAAGCAGTGTCTACGAACAATTATTGAAGGCACAAGAAGCTCCGGCTCCCACCTT
  GTCTTTGTCCCGTCATTGAGAGATGTGCACCATGAGCCTGTGTACCCCCAGCCGCCTTTCAGCTACTCCG
  ATCTGTCTCGAGAGGACAAAAAGCAAGTACAGTTTGTGTCCGAGCCCTGCAGCCTCTCCATAAACGGAGT
  GATCTTCGGCTTGACATCCACAGATCTGCTTTTCCACCTGGGGGCCGAGGAGATCAGTAGTTCTTCCGGA
  ACTTCAGACAGATTCAGCCGAATACTCAAGCACATCTTGACCCAGAGGAGCTACTACCCACTCTACCCGC
  CCCAAGAAGACATGGCCATTGACTATGAGTCGTTCTATGTTTACGCACAGCTGCCTGTCACCCCAGATGT
  CCTCATCATCCCGTCAGAGCTGAGGTACTTCGTGAAGGATGTCCTCGGCTGTGTCTGTGTGAACCCTGGG
  CGCCTTACCAAAGGGCAGGTGGGAGGCACCTTCGCCCGACTCTACCTTAGGAGGCCGGCAGCGGACGGGG
  CAGAGAGGCAGAGCCCATGCATTGCTGTGCAGGTCGTCAGGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201145 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSASAQQLAEELQIFGLDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFL
  SKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTR
  SPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMF
  QKLPDIREVLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGDREHSS
  GAQIPVDLSELKEYSLFPGQVVIMEGINTTGRKLVATKLYEGVPLPFYQPTEEDADFEQSMVLVACGPYT
  TSDSITYDPLLDLIAVINHDRPDVCILFGPFLDAKHEQVENCLLTSPFEDIFKQCLRTIIEGTRSSGSHL
  VFVPSLRDVHHEPVYPQPPFSYSDLSREDKKQVQFVSEPCSLSINGVIFGLTSTDLLFHLGAEEISSSSG
  TSDRFSRILKHILTQRSYYPLYPPQEDMAIDYESFYVYAQLPVTPDVLIIPSELRYFVKDVLGCVCVNPG
  RLTKGQVGGTFARLYLRRPAADGAERQSPCIAVQVVRI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002689
• ORF Size: 1794 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002689.1, NM_002689.2, NM_002689.3, NP_002680.2
• RefSeq Size: 2514 bp
• RefSeq ORF: 1797 bp
• Locus ID: 23649
• Domains: DNA_pol_alpha_B
• Protein Pathways: DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• MW: 65.9 kDa
• Gene Summary: May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery. [UniProtKB/Swiss-Prot Function]