UBE2G1 (NM_003342) Human Tagged ORF Clone
CAT#: RC201159
UBE2G1 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2G 1 (UBE2G1)
"NM_003342" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | UBE2G1 |
Synonyms | E217K; UBC7; UBE2G |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201159 representing NM_003342
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGAGCTGCAGTCGGCACTGCTACTGCGAAGACAGCTGGCAGAACTCAACAAAAATCCAGTGGAAG GCTTTTCTGCAGGTTTAATAGATGACAATGATCTCTACCGATGGGAAGTCCTTATTATTGGCCCTCCAGA TACACTTTATGAAGGTGGTGTTTTTAAGGCTCATCTTACTTTCCCAAAAGATTATCCCCTCCGACCTCCT AAAATGAAATTCATTACAGAAATCTGGCACCCAAATGTTGATAAAAATGGTGATGTGTGCATTTCTATTC TTCATGAGCCTGGGGAAGATAAGTATGGTTATGAAAAGCCAGAGGAACGCTGGCTCCCTATCCACACTGT GGAAACCATCATGATTAGTGTCATTTCTATGCTGGCAGACCCTAATGGAGACTCACCTGCTAATGTTGAT GCTGCGAAAGAATGGAGGGAAGATAGAAATGGAGAATTTAAAAGAAAAGTTGCCCGCTGTGTAAGAAAAA GCCAAGAGACTGCTTTTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201159 representing NM_003342
Red=Cloning site Green=Tags(s) MTELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPP KMKFITEIWHPNVDKNGDVCISILHEPGEDKYGYEKPEERWLPIHTVETIMISVISMLADPNGDSPANVD AAKEWREDRNGEFKRKVARCVRKSQETAFE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003342 |
ORF Size | 510 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003342.1, NM_003342.2, NM_003342.3, NM_003342.4, NP_003333.1 |
RefSeq Size | 4208 bp |
RefSeq ORF | 513 bp |
Locus ID | 7326 |
Cytogenetics | 17p13.2 |
Domains | UBCc |
Protein Pathways | Parkinson's disease, Ubiquitin mediated proteolysis |
MW | 19.3 kDa |
Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC112727 | UBE2G1 (untagged)-Human ubiquitin-conjugating enzyme E2G 1 (UBE2G1) |
USD 310.00 |
|
RG201159 | UBE2G1 (GFP-tagged) - Human ubiquitin-conjugating enzyme E2G 1 (UBE2G1) |
USD 460.00 |
|
RC201159L1 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2G 1 (UBE2G1), Myc-DDK-tagged |
USD 620.00 |
|
RC201159L2 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2G 1 (UBE2G1), mGFP tagged |
USD 768.00 |
|
RC201159L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2G 1 (UBE2G1), Myc-DDK-tagged |
USD 620.00 |
|
RC201159L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2G 1 (UBE2G1), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review