SUPT5H (NM_003169) Human Tagged ORF Clone

CAT#: RC201196

SUPT5H (Myc-DDK-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1

Product Images

Coomassie blue staining of purified SUPT5H protein (Cat# TP301196). The protein was produced from HEK293T cells transfected with SUPT5H cDNA clone (Cat# RC201196) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SUPT5H
• Synonyms: SPT5; SPT5H; Tat-CT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201196 representing NM_003169
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGACAGCGAGGACAGCAACTTTTCCGAGGAGGAGGACAGCGAGCGCAGCAGTGACGGCGAGGAGG
  CCGAGGTAGACGAAGAGCGGCGGAGTGCAGCGGGCAGTGAGAAAGAAGAAGAGCCTGAGGACGAAGAGGA
  GGAGGAAGAGGAGGAGGAATATGATGAGGAAGAGGAAGAAGAAGATGATGACCGACCCCCCAAGAAACCC
  CGCCATGGAGGCTTCATTCTGGACGAGGCTGATGTTGACGATGAGTATGAGGACGAGGACCAGTGGGAGG
  ATGGAGCAGAGGACATTCTAGAGAAAGAAGAGATTGAAGCCTCCAATATCGATAATGTTGTCCTGGATGA
  AGATCGTTCTGGGGCTCGCCGCCTGCAAAACCTCTGGAGGGACCAGCGAGAAGAAGAACTGGGCGAGTAT
  TACATGAAGAAATACGCCAAGTCATCTGTGGGAGAGACGGTGTATGGAGGATCTGATGAGCTCTCAGACG
  ACATCACCCAGCAGCAGCTGCTCCCAGGAGTCAAGGATCCCAATCTGTGGACTGTCAAATGTAAGATTGG
  GGAGGAACGGGCCACGGCCATTTCCTTGATGCGCAAGTTCATTGCCTACCAGTTCACAGACACGCCCCTG
  CAGATCAAGTCAGTAGTGGCACCAGAGCATGTGAAGGGCTACATCTACGTGGAGGCCTACAAGCAGACCC
  ACGTGAAGCAGGCCATTGAGGGGGTGGGCAACCTGCGGCTTGGCTACTGGAACCAGCAGATGGTGCCCAT
  CAAGGAGATGACAGACGTGCTCAAAGTGGTGAAGGAGGTGGCCAACCTGAAACCAAAGTCCTGGGTCCGC
  CTCAAGCGGGGCATCTACAAGGATGACATTGCTCAGGTGGACTACGTGGAGCCCAGCCAGAACACCATCT
  CCCTGAAGATGATCCCACGCATCGACTACGATCGCATCAAGGCCCGCATGAGCTTGAAAGACTGGTTTGC
  CAAAAGGAAGAAGTTTAAGCGGCCTCCACAGAGGCTGTTTGATGCTGAGAAGATCAGGTCCCTGGGGGGT
  GATGTTGCCTCTGATGGTGACTTCCTCATCTTTGAGGGGAACCGTTACAGCCGGAAGGGCTTTCTGTTCA
  AGAGCTTCGCCATGTCTGCTGTGATCACGGAGGGTGTGAAGCCAACACTCTCTGAGCTGGAAAAGTTTGA
  GGACCAGCCAGAGGGCATTGACCTGGAGGTGGTGACTGAGAGCACAGGGAAGGAGCGGGAGCACAACTTC
  CAACCTGGGGACAACGTGGAGGTCTGTGAGGGTGAGCTCATCAACCTGCAGGGCAAGATCCTCAGCGTGG
  ATGGCAACAAGATCACCATCATGCCCAAGCATGAGGACCTCAAGGACATGTTGGAGTTCCCAGCCCAGGA
  ACTTAGAAAATACTTCAAGATGGGGGACCACGTGAAGGTGATTGCTGGCCGATTCGAGGGCGACACAGGC
  CTCATTGTGCGGGTGGAGGAGAATTTCGTTATCCTGTTCTCTGACCTCACCATGCATGAGCTGAAGGTGC
  TCCCCCGGGACCTGCAGCTCTGCTCAGAGACAGCATCAGGTGTGGATGTTGGGGGCCAGCATGAATGGGG
  CGAGCTGGTGCAGCTGGATCCCCAGACTGTGGGTGTCATCGTGCGACTAGAACGGGAGACCTTCCAGGTG
  CTGAACATGTACGGGAAGGTGGTGACTGTCAGACATCAGGCTGTGACCCGGAAGAAGGACAACCGCTTTG
  CTGTGGCCTTGGACTCAGAGCAGAACAACATCCATGTGAAAGACATCGTTAAGGTCATTGATGGCCCCCA
  CTCAGGCCGAGAAGGGGAGATTCGCCATCTCTTCCGAAGCTTCGCCTTCCTACATTGCAAGAAACTGGTG
  GAGAACGGGGGCATGTTTGTCTGCAAGACCCGCCACCTGGTGCTGGCTGGGGGCTCAAAGCCCCGTGATG
  TGACCAACTTCACCGTGGGTGGCTTTGCGCCTATGAGTCCCCGGATCAGCAGCCCCATGCACCCCAGTGC
  TGGAGGTCAGCGTGGCGGCTTTGGTAGCCCAGGTGGCGGCAGTGGTGGCATGAGCAGGGGCCGGGGCCGG
  AGGGACAACGAACTCATCGGCCAGACCGTGCGCATCTCCCAGGGGCCCTACAAAGGCTACATCGGTGTGG
  TGAAAGATGCCACAGAGTCCACGGCCCGTGTGGAGCTGCACTCCACCTGCCAGACCATCTCTGTGGACCG
  TCAGCGGCTCACCACGGTGGGCTCACGGCGCCCGGGCGGCATGACCTCGACCTATGGGAGGACGCCCATG
  TATGGCTCCCAGACGCCCATGTATGGCTCTGGCTCCCGAACACCCATGTACGGCTCACAGACACCCCTCC
  AGGATGGTAGCCGCACCCCACACTACGGCTCACAGACGCCCCTGCATGATGGCAGCCGCACTCCTGCCCA
  GAGTGGGGCCTGGGACCCCAACAACCCCAACACGCCGTCACGGGCTGAGGAAGAATATGAGTATGCTTTC
  GATGATGAGCCCACCCCGTCCCCGCAGGCCTATGGGGGAACCCCCAATCCCCAAACACCTGGCTACCCAG
  ACCCCTCGTCCCCACAGGTCAACCCACAATACAACCCGCAGACGCCAGGGACGCCGGCCATGTACAACAC
  AGACCAGTTCTCTCCCTATGCTGCCCCCTCCCCACAAGGTTCCTACCAGCCCAGCCCCAGCCCCCAGAGC
  TACCACCAGGTGGCGCCAAGCCCAGCAGGCTACCAGAATACCCACTCCCCAGCCAGCTACCACCCTACAC
  CGTCGCCCATGGCCTATCAGGCTAGCCCCAGCCCGAGCCCCGTTGGCTACAGTCCTATGACACCTGGAGC
  TCCCTCCCCTGGTGGCTACAACCCACACACGCCAGGCTCAGGCATCGAGCAGAACTCCAGCGACTGGGTA
  ACCACTGACATTCAGGTGAAGGTGCGGGACACCTACCTGGATACACAGGTGGTGGGACAGACAGGTGTCA
  TCCGCAGTGTCACGGGGGGCATGTGCTCTGTGTACCTGAAGGACAGTGAGAAGGTTGTCAGCATTTCCAG
  TGAGCACCTGGAGCCTATCACCCCCACCAAGAACAACAAGGTGAAAGTGATCCTGGGCGAGGATCGGGAA
  GCCACGGGCGTCCTACTGAGCATTGATGGTGAGGATGGCATTGTCCGTATGGACCTTGATGAGCAGCTCA
  AGATCCTCAACCTCCGCTTCCTGGGGAAGCTCCTGGAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201196 representing NM_003169
  Red=Cloning site Green=Tags(s)
  
  MSDSEDSNFSEEEDSERSSDGEEAEVDEERRSAAGSEKEEEPEDEEEEEEEEEYDEEEEEEDDDRPPKKP
  RHGGFILDEADVDDEYEDEDQWEDGAEDILEKEEIEASNIDNVVLDEDRSGARRLQNLWRDQREEELGEY
  YMKKYAKSSVGETVYGGSDELSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFIAYQFTDTPL
  QIKSVVAPEHVKGYIYVEAYKQTHVKQAIEGVGNLRLGYWNQQMVPIKEMTDVLKVVKEVANLKPKSWVR
  LKRGIYKDDIAQVDYVEPSQNTISLKMIPRIDYDRIKARMSLKDWFAKRKKFKRPPQRLFDAEKIRSLGG
  DVASDGDFLIFEGNRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVVTESTGKEREHNF
  QPGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDMLEFPAQELRKYFKMGDHVKVIAGRFEGDTG
  LIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDVGGQHEWGELVQLDPQTVGVIVRLERETFQV
  LNMYGKVVTVRHQAVTRKKDNRFAVALDSEQNNIHVKDIVKVIDGPHSGREGEIRHLFRSFAFLHCKKLV
  ENGGMFVCKTRHLVLAGGSKPRDVTNFTVGGFAPMSPRISSPMHPSAGGQRGGFGSPGGGSGGMSRGRGR
  RDNELIGQTVRISQGPYKGYIGVVKDATESTARVELHSTCQTISVDRQRLTTVGSRRPGGMTSTYGRTPM
  YGSQTPMYGSGSRTPMYGSQTPLQDGSRTPHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAF
  DDEPTPSPQAYGGTPNPQTPGYPDPSSPQVNPQYNPQTPGTPAMYNTDQFSPYAAPSPQGSYQPSPSPQS
  YHQVAPSPAGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWV
  TTDIQVKVRDTYLDTQVVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDRE
  ATGVLLSIDGEDGIVRMDLDEQLKILNLRFLGKLLEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003169
• ORF Size: 3261 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003169.1, NM_003169.2, NM_003169.3, NP_003160.2
• RefSeq Size: 3762 bp
• RefSeq ORF: 3264 bp
• Locus ID: 6829
• Cytogenetics: 19q13.2
• Domains: Supt5, KOW, NGN, KOW
• Protein Families: Transcription Factors
• MW: 120.8 kDa
• Gene Summary: ''