SFRS4 (SRSF4) (NM_005626) Human Tagged ORF Clone

CAT#: RC201227

SRSF4 (Myc-DDK-tagged)-Human serine/arginine-rich splicing factor 4 (SRSF4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRSF4
• Synonyms: SFRS4; SRP75
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201227 representing NM_005626
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCGGGTGTACATCGGCCGCCTGAGCTACCAGGCCCGGGAGCGCGATGTGGAGCGCTTCTTTAAGG
  GCTACGGGAAGATCCTGGAGGTGGATCTGAAGAACGGATATGGTTTTGTGGAGTTTGATGATCTGCGTGA
  TGCAGATGATGCTGTTTATGAACTGAATGGCAAAGACCTTTGTGGTGAGCGAGTAATTGTTGAGCATGCC
  CGCGGCCCACGGCGAGATGGCAGTTACGGTTCTGGACGCAGTGGATATGGTTATAGAAGAAGTGGCCGAG
  ATAAATATGGCCCTCCTACTCGCACAGAGTACAGACTTATTGTGGAGAATTTGTCAAGTCGGTGCAGCTG
  GCAAGACCTAAAGGATTATATGCGTCAGGCAGGAGAAGTGACTTATGCAGATGCTCACAAGGGACGCAAA
  AATGAAGGGGTGATTGAATTTGTATCTTATTCTGATATGAAAAGAGCTTTGGAAAAGTTGGATGGAACTG
  AAGTCAATGGGAGAAAAATCAGATTAGTTGAAGACAAGCCAGGTTCCAGACGACGCCGGTCCTACTCCAG
  AAGCCGGAGTCATTCAAGGTCTCGCTCTCGAAGCAGACATTCCCGTAAGAGCAGAAGCCGAAGTGGCAGC
  AGCAAAAGCAGTCATTCTAAGAGTAGATCTCGGTCCAGGTCGGGCTCCCGCTCCCGGAGCAAGAGCCGGA
  GCCGGAGCCAGAGTCGGAGCCGGAGCAAGAAAGAGAAAAGCAGGAGCCCCAGCAAGGAAAAGAGCCGCAG
  CCGCAGCCATAGCGCTGGCAAGAGCCGCAGCAAGAGCAAAGACCAAGCTGAAGAGAAGATCCAAAACAAT
  GACAATGTCGGGAAACCCAAGAGCCGGAGTCCTAGCAGGCATAAAAGTAAGAGCAAAAGTCGGAGCAGGA
  GTCAGGAGAGGAGAGTGGAGGAGGAGAAGCGAGGGAGTGTGAGCAGGGGCAGGAGCCAGGAGAAGAGCCT
  CCGCCAGAGTCGGAGCCGGAGCAGGAGCAAAGGGGGCAGCAGGAGCCGGAGCAGGAGCCGCAGCAAGAGC
  AAGGACAAGAGGAAGGGCAGGAAGAGAAGCAGAGAGGAGAGCCGCAGTCGCAGTCGCAGCCGCAGCAAGA
  GTGAGAGGAGCAGAAAGCGAGGCAGCAAGCGAGACAGCAAGGCGGGCAGCAGCAAGAAGAAGAAGAAGGA
  AGACACTGACCGCTCCCAGTCCAGATCTCCATCCCGCTCCGTGTCAAAGGAGCGGGAACATGCCAAGTCT
  GAATCCAGCCAGAGGGAAGGTCGAGGAGAGAGTGAGAATGCTGGCACCAATCAGGAGACCCGGTCCAGGT
  CGAGATCCAATTCCAAATCGAAACCAAACCTTCCATCAGAATCACGCTCCAGATCAAAGTCAGCTTCAAA
  AACCCGATCTCGGTCCAAGTCTAGATCCAGGTCTGCTTCCAGATCGCCCTCCCGATCTAGATCTAGGTCC
  CACTCAAGGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201227 representing NM_005626
  Red=Cloning site Green=Tags(s)
  
  MPRVYIGRLSYQARERDVERFFKGYGKILEVDLKNGYGFVEFDDLRDADDAVYELNGKDLCGERVIVEHA
  RGPRRDGSYGSGRSGYGYRRSGRDKYGPPTRTEYRLIVENLSSRCSWQDLKDYMRQAGEVTYADAHKGRK
  NEGVIEFVSYSDMKRALEKLDGTEVNGRKIRLVEDKPGSRRRRSYSRSRSHSRSRSRSRHSRKSRSRSGS
  SKSSHSKSRSRSRSGSRSRSKSRSRSQSRSRSKKEKSRSPSKEKSRSRSHSAGKSRSKSKDQAEEKIQNN
  DNVGKPKSRSPSRHKSKSKSRSRSQERRVEEEKRGSVSRGRSQEKSLRQSRSRSRSKGGSRSRSRSRSKS
  KDKRKGRKRSREESRSRSRSRSKSERSRKRGSKRDSKAGSSKKKKKEDTDRSQSRSPSRSVSKEREHAKS
  ESSQREGRGESENAGTNQETRSRSRSNSKSKPNLPSESRSRSKSASKTRSRSKSRSRSASRSPSRSRSRS
  HSRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005626
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005626.1, NM_005626.2, NM_005626.3, NM_005626.4, NP_005617.2
• RefSeq Size: 2158 bp
• RefSeq ORF: 1485 bp
• Locus ID: 6429
• Cytogenetics: 1p35.3
• Domains: RRM
• Protein Pathways: Spliceosome
• MW: 56.5 kDa
• Gene Summary: 'This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing. [provided by RefSeq, Feb 2009]'