MCPIP1 (ZC3H12A) (NM_025079) Human Tagged ORF Clone

CAT#: RC201381

ZC3H12A (Myc-DDK-tagged)-Human zinc finger CCCH-type containing 12A (ZC3H12A)

Product Images

Western blot validation of overexpression lysate (Cat# LY410904) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201381 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ZC3H12A protein (Cat# TP301381). The protein was produced from HEK293T cells transfected with ZC3H12A cDNA clone (Cat# RC201381) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZC3H12A
• Synonyms: dJ423B22.1; MCPIP; MCPIP-1; MCPIP1; Reg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201381 representing NM_025079.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGTGGCCCCTGTGGAGAGAAGCCTGTCCTGGAAGCCAGCCCCACCATGAGTCTGTGGGAATTTGAG
  GACAGCCACAGCCGTCAGGGCACCCCAAGGCCGGGTCAAGAGCTGGCCGCTGAGGAGGCCTCGGCCCTG
  GAACTGCAGATGAAGGTGGACTTCTTCCGGAAGCTGGGCTATTCATCCACGGAGATCCACAGCGTCCTG
  CAGAAGCTGGGCGTCCAGGCAGACACCAACACGGTGCTGGGTGAGCTGGTGAAACACGGGACAGCCACC
  GAGCGGGAGCGCCAGACCTCACCGGACCCCTGCCCTCAGCTCCCTCTAGTCCCGCGGGGTGGTGGCACC
  CCTAAGGCTCCCAACCTGGAGCCTCCACTCCCAGAAGAGGAAAAGGAGGGCAGCGACCTGAGACCAGTG
  GTCATCGATGGGAGCAACGTGGCCATGAGCCATGGGAACAAGGAGGTCTTCTCCTGCCGGGGCATCCTG
  CTGGCAGTGAACTGGTTTCTGGAGCGGGGCCACACAGACATCACAGTGTTTGTGCCATCCTGGAGGAAG
  GAGCAGCCTCGGCCCGACGTGCCCATCACAGACCAGCACATCCTGCGGGAACTGGAGAAGAAGAAGATC
  CTGGTGTTCACACCATCACGACGCGTGGGTGGCAAGCGGGTGGTGTGCTATGACGACAGATTCATTGTG
  AAGCTGGCCTACGAGTCTGACGGGATCGTGGTTTCCAACGACACATACCGTGACCTCCAAGGCGAGCGG
  CAGGAGTGGAAGCGCTTCATCGAGGAGCGGCTGCTCATGTACTCCTTCGTCAATGACAAGTTTATGCCC
  CCTGATGACCCACTGGGCCGGCACGGGCCCAGCCTGGACAACTTCCTGCGTAAGAAGCCACTCACTTTG
  GAGCACAGGAAGCAGCCGTGTCCCTATGGAAGGAAATGCACCTATGGGATCAAGTGCCGATTCTTCCAC
  CCAGAGCGGCCAAGCTGCCCCCAGCGCTCTGTGGCAGATGAGCTCCGTGCCAATGCTCTCCTCTCACCC
  CCCAGAGCCCCAAGCAAGGACAAAAATGGCCGGCGGCCTTCACCTTCATCCCAGTCCAGCTCTCTGCTA
  ACAGAGAGTGAGCAGTGCAGCCTGGATGGGAAGAAGCTGGGGGCCCAGGCATCCCCAGGGTCCCGCCAA
  GAGGGTCTAACACAGACCTATGCCCCATCAGGCAGGAGCCTCGCACCTAGCGGGGGCAGTGGCAGCAGC
  TTTGGGCCCACAGACTGGCTCCCACAGACGCTGGACTCACTCCCGTACGTCTCCCAGGATTGCCTGGAC
  TCGGGCATTGGCTCCCTGGAGAGCCAGATGTCGGAACTTTGGGGGGTTCGAGGAGGAGGCCCTGGTGAG
  CCGGGCCCACCCCGAGCCCCTTACACGGGCTACAGTCCCTATGGATCTGAGCTCCCAGCCACCGCAGCC
  TTCTCTGCCTTTGGCCGGGCCATGGGTGCTGGCCACTTCAGTGTCCCTGCCGACTACCCACCCGCGCCC
  CCTGCCTTTCCACCTCGAGAGTACTGGTCTGAACCATACCCACTGCCCCCACCCACATCAGTCCTTCAG
  GAGCCCCCAGTGCAGAGCCCAGGGGCTGGCAGGAGCCCGTGGGGCAGGGCAGACAGCCTGGCCAAGGAG
  CAGGCCAGCGTGTATACTAAGCTGTGTGGTGTGTTTCCCCCGCACCTGGTGGAGGCTGTGATGGGGCGC
  TTCCCACAGCTCCTGGACCCCCAGCAGCTGGCTGCCGAGATCCTCTCCTACAAGTCCCAGCACCCCAGT
  GAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC201381
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSGPCGEKPVLEASPTMSLWEFEDSHSRQGTPRPGQELAAEEASALELQMKVDFFRKLGYSSTEIHSVL
  QKLGVQADTNTVLGELVKHGTATERERQTSPDPCPQLPLVPRGGGTPKAPNLEPPLPEEEKEGSDLRPV
  VIDGSNVAMSHGNKEVFSCRGILLAVNWFLERGHTDITVFVPSWRKEQPRPDVPITDQHILRELEKKKI
  LVFTPSRRVGGKRVVCYDDRFIVKLAYESDGIVVSNDTYRDLQGERQEWKRFIEERLLMYSFVNDKFMP
  PDDPLGRHGPSLDNFLRKKPLTLEHRKQPCPYGRKCTYGIKCRFFHPERPSCPQRSVADELRANALLSP
  PRAPSKDKNGRRPSPSSQSSSLLTESEQCSLDGKKLGAQASPGSRQEGLTQTYAPSGRSLAPSGGSGSS
  FGPTDWLPQTLDSLPYVSQDCLDSGIGSLESQMSELWGVRGGGPGEPGPPRAPYTGYSPYGSELPATAA
  FSAFGRAMGAGHFSVPADYPPAPPAFPPREYWSEPYPLPPPTSVLQEPPVQSPGAGRSPWGRADSLAKE
  QASVYTKLCGVFPPHLVEAVMGRFPQLLDPQQLAAEILSYKSQHPSE
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC201381 also available, TP301381M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025079
• ORF Size: 1797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025079.1, NP_079355.1
• RefSeq Size: 2697 bp
• RefSeq ORF: 1800 bp
• Locus ID: 80149
• UniProt ID: Q5D1E8
• Cytogenetics: 1p34.3
• MW: 65.8 kDa
• Gene Summary: ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.[supplied by OMIM, Mar 2008]