Lenti RapidTiter Sticks

CAT#: EB200001

Lateral flow testing sticks used for the semi-quantitative detection of the lentiviral p24 protein, 5 reactions

Specifications

Product Data

• Gene: HIV-1 gag
• Assay Format: Sticks
• Assay Length: 10 minutes
• Signal: Color Band
• Detection Range: 2.5 x 10⁵ to 2.5 x 10⁷ TU/ml
• Sample Type: Cell cuture supernatant, lentiviral sample
• Sample Volume: 20µl of lentiviral sample
• Reactivities: p24 protein
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Background: The gag protein p24 (MW 24kD) is a major structural protein of the HIV capsid, which is required for virus particle assembly. There are approximately 2000 p24 molecules per virus particle, or at a molecule weight of 24 kDa, about 104 virus particles per picogram of p24. HIV-1 p24 measurement is commonly used as an indicator of HIV-1 infection and viral load. Quantitative detection of the HIV-1 p24 has become a standard method for measuring lentiviral titer.
• Components:
  • 5 Lenti RapidTiter Sticks
  • 1 Vial of Running Buffer (1.0ml/vial)
  • 1 Reference Card
• Quality Control: Lenti RapidTiter Sticks were validated by testing various dilutions of lentiviral samples with a known titer (measured by ELISA). The results were then compared to a developed reference card and the line density was confirmed to properly reflect the estimated titer of the samples.
• Storage: Upon receipt, store at room temperature
• Stability: The kit should not be used beyond the expiration date. Leave Lenti RapidTiter Sticks sealed in its foil pouch until just before use. Do not use if pouch is damaged or open. Once opened, the Lenti RapidTiter Stick should be used immediately.