DPH2 (NM_001384) Human Tagged ORF Clone
CAT#: RC201382
DPH2 (Myc-DDK-tagged)-Human DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 1
"NM_001384" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | DPH2 |
Synonyms | DPH2L2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201382 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGATGTTTAGCAGCCCTGCCGAGGCGGCGCTGCAGCGAGAGACCGGGGTGCCAGGACTGCTTA CTCCTCTTCCGGACCTGGACGGAGTGTACGAGCTGGAGCGAGTCGCTGGATTTGTCCGCGACCTGGGGTG TGAACGAGTTGCCTTGCAGTTCCCTGACCAGCTATTGGGAGATGCTGTGGCTGTGGCTGCACGACTGGAG GAGACGACAGGGTCAAAGATGTTCATTCTGGGTGACACAGCCTACGGCAGCTGCTGCGTGGATGTGCTGG GTGCTGAGCAAGCTGGAGCTCAGGCTCTCATACATTTTGGCCCTGCCTGCTTAAGCCCTCCAGCCCGCCC ACTGCCCGTTGCCTTCGTGCTTCGTCAACGTTCTGTGGCCTTGGAGCTCTGTGTCAAGGCCTTTGAGGCC CAGAACCCAGACCCCAAAGCGCCTGTGGTGCTGCTGAGTGAGCCGGCCTGTGCCCATGCCCTGGAGGCTT TGGCTACTCTCCTGCGCCCACGGTACCTGGACCTGCTAGTCTCCAGCCCAGCTTTTCCCCAACCAGTGGG TTCCCTGAGTCCAGAGCCTATGCCCCTAGAGCGTTTTGGGCGCCGCTTCCCCCTTGCCCCAGGGAGGCGT CTAGAAGAGTATGGTGCCTTCTATGTAGGGGGCTCTAAGGCCAGCCCTGACCCAGACCTTGACCCAGACC TGAGTCGGCTGCTCTTGGGGTGGGCACCAGGTCAACCCTTCTCCTCCTGCTGTCCAGATACAGGGAAGAC TCAGGATGAGGGTGCCCGGGCTGGACGGCTAAGGGCACGAAGACGATATCTGGTAGAGAGGGCCAGAGAT GCCCGCGTGGTAGGGCTGCTGGCAGGCACACTGGGTGTAGCCCAACACCGTGAGGCACTGGCCCACTTGC GGAACCTGACTCAGGCTGCTGGCAAGCGTAGCTATGTGTTGGCCCTGGGGCGGCCCACCCCTGCCAAGCT TGCCAACTTCCCTGAGGTGGATGTCTTTGTGCTATTAGCCTGTCCTCTGGGTGCTCTAGCCCCCCAGCTT TCTGGTAGCTTCTTCCAGCCTATACTGGCACCATGTGAGCTGGAAGCTGCCTGCAACCCTGCCTGGCCAC CTCCAGGCCTGGCTCCCCACCTCACACATTATGCGGACTTATTGCCTGGCTCTCCCTTCCACGTGGCTCT CCCACCACCTGAGTCAGAGCTGTGGGAAACCCCAGACGTGTCACTCATTACTGGAGATCTCCGACCCCCA CCTGCCTGGAAGTCATCAAATGATCATGGAAGCTTGGCTCTGACCCCACGGCCCCAGCTGGAGCTGGCTG AGAGCAGTCCTGCAGCCTCATTCCTTAGTTCCCGGAGCTGGCAAGGGCTGGAGCCCCGCCTGGGTCAGAC GCCAGTGACAGAAGCTGTGAGTGGAAGACGAGGGATTGCCATCGCCTATGAGGATGAGGGAAGCGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201382 protein sequence
Red=Cloning site Green=Tags(s) MESMFSSPAEAALQRETGVPGLLTPLPDLDGVYELERVAGFVRDLGCERVALQFPDQLLGDAVAVAARLE ETTGSKMFILGDTAYGSCCVDVLGAEQAGAQALIHFGPACLSPPARPLPVAFVLRQRSVALELCVKAFEA QNPDPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRR LEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARD ARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQL SGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPP PAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001384 |
ORF Size | 1467 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001384.2, NM_001384.3, NM_001384.4, NP_001375.2 |
RefSeq Size | 2513 bp |
RefSeq ORF | 1470 bp |
Locus ID | 1802 |
Cytogenetics | 1p34.1 |
Domains | Diphthamide_syn |
MW | 52.1 kDa |
Gene Summary | 'This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC321580 | DPH2 (untagged)-Human DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 1 |
USD 760.00 |
|
RG201382 | DPH2 (GFP-tagged) - Human DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 1 |
USD 460.00 |
|
RC201382L3 | Lenti ORF clone of Human DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC201382L4 | Lenti ORF clone of Human DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 1, mGFP tagged |
USD 620.00 |
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