DPH2 (NM_001384) Human Untagged Clone

CAT#: SC321580

DPH2 (untagged)-Human DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DPH2
• Synonyms: DPH2L2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001384.4 AGAAACAGTAGTTAGGATGGCTGAAGGGGATACTCACCGGCTGAAGGCCGACTGTGATTC
  CCCCTACCCCCACAAGGCGATTTTGACCCCCTGAGGGCTGCTCTAGAGGACTCAGGCCCC
  GAAGCTGTCCCAGGGAGGTCCCCGCTGCATCCCACCACCCAAGCTGTGCCTCATGGAGTC
  GATGTTTAGCAGCCCTGCCGAGGCGGCGCTGCAGCGAGAGACCGGGGTGCCAGGACTGCT
  TACTCCTCTTCCGGACCTGGACGGAGTGTACGAGCTGGAGCGAGTCGCTGGATTTGTCCG
  CGACCTGGGGTGTGAACGAGTTGCCTTGCAGTTCCCTGACCAGCTATTGGGAGATGCTGT
  GGCTGTGGCTGCACGACTGGAGGAGACGACAGGGTCAAAGATGTTCATTCTGGGTGACAC
  AGCCTACGGCAGCTGCTGCGTGGATGTGCTGGGTGCTGAGCAAGCTGGAGCTCAGGCTCT
  CATACATTTTGGCCCTGCCTGCTTAAGCCCTCCAGCCCGCCCACTGCCCGTTGCCTTCGT
  GCTTCGTCAACGTTCTGTGGCCTTGGAGCTCTGTGTCAAGGCCTTTGAGGCCCAGAACCC
  AGACCCCAAAGCGCCTGTGGTGCTGCTGAGTGAGCCGGCCTGTGCCCATGCCCTGGAGGC
  TTTGGCTACTCTCCTGCGCCCACGGTACCTGGACCTGCTAGTCTCCAGCCCAGCTTTTCC
  CCAACCAGTGGGTTCCCTGAGTCCAGAGCCTATGCCCCTAGAGCGTTTTGGGCGCCGCTT
  CCCCCTTGCCCCAGGGAGGCGTCTAGAAGAGTATGGTGCCTTCTATGTAGGGGGCTCTAA
  GGCCAGCCCTGACCCAGACCTTGACCCAGACCTGAGTCGGCTGCTCTTGGGGTGGGCACC
  AGGTCAACCCTTCTCCTCCTGCTGTCCAGATACAGGGAAGACTCAGGATGAGGGTGCCCG
  GGCTGGACGGCTAAGGGCACGAAGACGATATCTGGTAGAGAGGGCCAGAGATGCCCGCGT
  GGTAGGGCTGCTGGCAGGCACACTGGGTGTAGCCCAACACCGTGAGGCACTGGCCCACTT
  GCGGAACCTGACTCAGGCTGCTGGCAAGCGTAGCTATGTGTTGGCCCTGGGGCGGCCCAC
  CCCTGCCAAGCTTGCCAACTTCCCTGAGGTGGATGTCTTTGTGCTATTAGCCTGTCCTCT
  GGGTGCTCTAGCCCCCCAGCTTTCTGGTAGCTTCTTCCAGCCTATACTGGCACCATGTGA
  GCTGGAAGCTGCCTGCAACCCTGCCTGGCCACCTCCAGGCCTGGCTCCCCACCTCACACA
  TTATGCGGACTTATTGCCTGGCTCTCCCTTCCACGTGGCTCTCCCACCACCTGAGTCAGA
  GCTGTGGGAAACCCCAGACGTGTCACTCATTACTGGAGATCTCCGACCCCCACCTGCCTG
  GAAGTCATCAAATGATCATGGAAGCTTGGCTCTGACCCCACGGCCCCAGCTGGAGCTGGC
  TGAGAGCAGTCCTGCAGCCTCATTCCTTAGTTCCCGGAGCTGGCAAGGGCTGGAGCCCCG
  CCTGGGTCAGACGCCAGTGACAGAAGCTGTGAGTGGAAGACGAGGGATTGCCATCGCCTA
  TGAGGATGAGGGAAGCGGCTGATACCATGTGGGGCTGGAGACATAGATGGACTTATGAAT
  GGCTGCTAGGACCTTTAGTGCTCCCTGCACCAACCTCCCATCCCCCTGCCAAGATCCTTG
  AAGGACCCTGGAAGGAGGGAGAGCAGGCAGCCCTTCACAGGATAGGATCCGTCTCTGTCC
  TGTCCTGGCACTGGCACAAGCTCAGCCCATGCCCAGTAATGCGTGTTGTTTGGCTGATGG
  AATAAAGGGCTTAGGGACTTCCCTGAGGCCTCTGGACCCATCTGTCTTCCTGAGGGCAGC
  CCAGGACCTTTGGCCAATCCCAGTTCCCAGGCTGCAGTTGAGGGTCTGTCCTTGTCAAAA
  GGCAGGTGCTAGACAGTCTAGACCAGGGTTTCTCAAACTCGTACTTGACATTTGGGGCCA
  GATAATTCTTTGTTGTGGGGCTGTCTGGTGTATGGTAGGGTGCTCAGCAGCATCCCTGGC
  CTCTGCCCACTAGACATCAGAAGCACTCCCCCAGTTGTGACAACCAAAAATATCTCCAGA
  CCTTGGCAAATGTTATCTGTGGGGGAAAATTGCCCTCAATTGAGAACCACTGGTCTAGCT
  AGACCTGCACTGTCCAGTACAGTAGCCACTAAATACATGTGGCTAAACTTAAATTTAAGT
  TAATTAAGATTAAAAGCTCAGTTTCTCAGTCACATTAGTCATTCAAGTGTTCAGACAGCC
  ACATGAGGGGACAGTGCAGCTACAGGATATGCCATCATGGCAGAAAGTTCTGTTGGTTGG
  ACAGCTTTGGTCTATACTGACTCTTATTTCTCAGGGAGATCACAGCAACCTAAATAAACC
  AGATACCTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001384
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001384.4, NP_001375.2
• RefSeq Size: 2513 bp
• RefSeq ORF: 1470 bp
• Locus ID: 1802
• Cytogenetics: 1p34.1
• Domains: Diphthamide_syn
• Gene Summary: 'This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) encodes the longest isoform (a).