BXDC2 (BRIX1) (NM_018321) Human Tagged ORF Clone

CAT#: RC201485

BRIX1 (Myc-DDK-tagged)-Human BRX1, biogenesis of ribosomes, homolog (S. cerevisiae) (BRIX1)

Product Images

Western blot validation of overexpression lysate (Cat# LY413164) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201485 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRIX1
• Synonyms: BRIX; BXDC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201485 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAACCAAGAGGAAACGGCGTGGAGGCTTTGCAGTTCAGGCGAAGAAGCCAAAAAGAAACGAAA
  TAGATGCGGAGCCGCCAGCTAAGCGGCACGCCACAGCAGAGGAGGTGGAGGAAGAAGAGAGGGACCGGAT
  CCCAGGCCCCGTTTGCAAGGGAAAGTGGAAAAATAAGGAACGGATTCTCATCTTTTCTTCCAGAGGAATA
  AATTTTAGAACAAGACATTTAATGCAGGACTTGAGAATGTTGATGCCTCATTCTAAAGCAGATACTAAAA
  TGGATCGTAAGGATAAGCTATTTGTGATTAACGAGGTTTGTGAAATGAAGAACTGTAATAAATGCATCTA
  TTTTGAAGCTAAGAAAAAACAGGATCTCTATATGTGGCTTTCAAATTCACCTCACGGACCATCTGCTAAA
  TTCCTTGTTCAAAATATTCATACCCTCGCTGAACTGAAGATGACTGGAAACTGTTTGAAAGGTTCTCGGC
  CCCTTTTGTCTTTTGACCCTGCTTTTGATGAATTACCACATTATGCTTTGTTAAAAGAACTCTTAATTCA
  GATCTTTAGTACACCACGGTATCATCCCAAAAGCCAACCATTTGTGGACCACGTGTTTACTTTCACCATT
  TTGGATAATAGGATATGGTTTCGGAACTTTCAGATCATAGAAGAAGATGCTGCTCTTGTAGAAATAGGAC
  CTCGTTTTGTCTTAAATCTCATAAAGATTTTCCAGGGAAGTTTTGGAGGACCAACTTTATATGAAAATCC
  TCACTACCAGTCACCAAACATGCATCGGCGTGTCATAAGATCCATCACAGCTGCAAAATACAGAGAGAAA
  CAGCAAGTGAAAGATGTGCAAAAACTGAGAAAGAAAGAGCCGAAGACTCTTCTTCCACATGATCCCACTG
  CAGATGTTTTTGTAACACCAGCTGAGGAGAAACCAATAGAAATACAGTGGGTAAAACCAGAGCCAAAAGT
  TGATTTGAAAGCAAGAAAGAAACGGATTTACAAAAGGCAAAGAAAAATGAAACAGAGGATGGACAGTGGG
  AAAACAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201485 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATKRKRRGGFAVQAKKPKRNEIDAEPPAKRHATAEEVEEEERDRIPGPVCKGKWKNKERILIFSSRGI
  NFRTRHLMQDLRMLMPHSKADTKMDRKDKLFVINEVCEMKNCNKCIYFEAKKKQDLYMWLSNSPHGPSAK
  FLVQNIHTLAELKMTGNCLKGSRPLLSFDPAFDELPHYALLKELLIQIFSTPRYHPKSQPFVDHVFTFTI
  LDNRIWFRNFQIIEEDAALVEIGPRFVLNLIKIFQGSFGGPTLYENPHYQSPNMHRRVIRSITAAKYREK
  QQVKDVQKLRKKEPKTLLPHDPTADVFVTPAEEKPIEIQWVKPEPKVDLKARKKRIYKRQRKMKQRMDSG
  KTK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018321
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018321.2, NM_018321.3, NP_060791.3
• RefSeq Size: 1303 bp
• RefSeq ORF: 1062 bp
• Locus ID: 55299
• Domains: Brix
• Protein Families: Embryonic stem cells, Stem cell - Pluripotency
• MW: 41.4 kDa
• Gene Summary: Required for biogenesis of the 60S ribosomal subunit. [UniProtKB/Swiss-Prot Function]