ELP3 (NM_018091) Human Tagged ORF Clone

CAT#: RC201491

ELP3 (Myc-DDK-tagged)-Human elongation protein 3 homolog (S. cerevisiae) (ELP3)


  "NM_018091" in other vectors (8)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ELP3
Synonyms KAT9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201491 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCAGAAGCGGAAAGGAGATCTCAGCCCTGCTGAGCTGATGATGCTGACTATAGGAGATGTTATTA
AACAACTGATTGAAGCCCACGAGCAGGGGAAAGACATCGATCTAAATAAGGTGAAAACCAAGACAGCTGC
CAAATATGGCCTTTCTGCCCAGCCCCGCCTGGTGGATATCATTGCTGCCGTCCCTCCTCAGTATCGCAAG
GTCTTGATGCCCAAGTTAAAGGCGAAACCCATCAGAACTGCTAGTGGGATTGCTGTCGTGGCTGTGATGT
GCAAACCCCACAGATGTCCACACATCAGTTTTACAGGAAATATATGTGTATACTGCCCTGGTGGACCTGA
TTCTGATTTTGAGTATTCCACCCAGTCTTACACTGGCTATGAGCCAACCTCCATGAGAGCTATCCGTGCC
AGATATGACCCTTTCCTACAGACAAGACACCGAATAGAACAGTTAAAACAACTTGGTCATAGTGTGGATA
AAGTGGAGTTTATTGTGATGGGTGGAACGTTTATGGCCCTTCCAGAAGAATACAGAGATTATTTTATTCG
AAATTTACATGATGCCTTATCAGGACATACTTCCAACAATATTTACGAGGCAGTCAAGTATTCTGAGAGA
AGCCTCACAAAGTGTATTGGAATTACTATTGAAACCAGACCAGATTACTGCATGAAGCGACATTTAAGTG
ACATGTTGACCTATGGCTGCACAAGGCTGGAGATTGGGGTGCAGAGTGTTTATGAAGATGTGGCTAGAGA
CACCAACAGGGGCCACACTGTGAAGGCAGTGTGTGAGTCATTTCACCTGGCCAAAGATTCCGGTTTTAAA
GTGGTGGCCCATATGATGCCTGACCTGCCAAACGTGGGACTAGAAAGAGACATTGAACAGTTCACAGAGT
TTTTTGAGAACCCTGCTTTTCGTCCCGATGGGCTGAAACTCTATCCTACCCTGGTGATTCGTGGGACCGG
GCTTTATGAGCTTTGGAAATCAGGAAGATATAAGAGTTACTCTCCTAGTGACCTGGTTGAATTGGTGGCT
CGGATCCTAGCCCTCGTGCCTCCATGGACTCGAGTGTACCGAGTACAGAGGGATATTCCAATGCCTTTAG
TTAGCTCAGGAGTAGAGCATGGTAACCTGAGAGAGCTGGCACTTGCAAGAATGAAAGACCTCGGAATACA
GTGTCGAGATGTGAGAACCAGAGAAGTTGGAATCCAAGAAATTCATCACAAAGTACGGCCATACCAGGTT
GAATTGGTAAGGAGAGATTATGTTGCAAATGGTGGCTGGGAAACATTCTTGTCATACGAAGACCCAGATC
AAGACATTTTGATTGGCCTCCTACGATTACGCAAGTGTTCAGAAGAAACTTTCCGTTTCGAATTGGGTGG
AGGTGTCTCCATAGTACGAGAGCTGCATGTGTATGGGAGTGTGGTCCCTGTGAGCAGCCGGGATCCTACT
AAATTTCAGCATCAGGGATTTGGCATGCTGCTGATGGAGGAAGCAGAAAGAATAGCTAGAGAAGAACATG
GGTCTGGGAAAATCGCTGTGATATCAGGGGTCGGCACCAGGAATTATTATAGAAAGATCGGCTACAGATT
ACAAGGCCCGTACATGGTGAAGATGCTGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201491 protein sequence
Red=Cloning site Green=Tags(s)

MRQKRKGDLSPAELMMLTIGDVIKQLIEAHEQGKDIDLNKVKTKTAAKYGLSAQPRLVDIIAAVPPQYRK
VLMPKLKAKPIRTASGIAVVAVMCKPHRCPHISFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRA
RYDPFLQTRHRIEQLKQLGHSVDKVEFIVMGGTFMALPEEYRDYFIRNLHDALSGHTSNNIYEAVKYSER
SLTKCIGITIETRPDYCMKRHLSDMLTYGCTRLEIGVQSVYEDVARDTNRGHTVKAVCESFHLAKDSGFK
VVAHMMPDLPNVGLERDIEQFTEFFENPAFRPDGLKLYPTLVIRGTGLYELWKSGRYKSYSPSDLVELVA
RILALVPPWTRVYRVQRDIPMPLVSSGVEHGNLRELALARMKDLGIQCRDVRTREVGIQEIHHKVRPYQV
ELVRRDYVANGGWETFLSYEDPDQDILIGLLRLRKCSEETFRFELGGGVSIVRELHVYGSVVPVSSRDPT
KFQHQGFGMLLMEEAERIAREEHGSGKIAVISGVGTRNYYRKIGYRLQGPYMVKMLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018091
ORF Size 1641 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018091.2, NM_018091.3, NM_018091.4, NM_018091.5, NP_060561.3
RefSeq Size 3232 bp
RefSeq ORF 1644 bp
Locus ID 55140
Domains Acetyltransf, Elp3, Radical_SAM
MW 62.3 kDa
Gene Summary ELP3 is the catalytic subunit of the histone acetyltransferase elongator complex, which contributes to transcript elongation and also regulates the maturation of projection neurons (Creppe et al., 2009 [PubMed 19185337]). [supplied by OMIM, Apr 2009]

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