ACAD8 (NM_014384) Human Tagged ORF Clone

CAT#: RC201507

ACAD8 (Myc-DDK-tagged)-Human acyl-CoA dehydrogenase family, member 8 (ACAD8), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY415328) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201507 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ACAD8 protein (Cat# TP301507). The protein was produced from HEK293T cells transfected with ACAD8 cDNA clone (Cat# RC201507) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACAD8
• Synonyms: ACAD-8; ARC42
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201507 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGAGCGGCTGCCGGCGTTTCGGGGCGCGCCTCGGCTGCCTGCCCGGCGGTCTCCGGGTCCTCG
  TCCAGACCGGCCACCGGAGCTTGACCTCCTGCATCGACCCTTCCATGGGACTTAATGAAGAGCAGAAAGA
  ATTTCAAAAAGTGGCCTTTGACTTTGCTGCCCGAGAGATGGCTCCAAATATGGCAGAGTGGGACCAGAAG
  GAGCTGTTCCCAGTGGATGTGATGCGGAAGGCAGCCCAGCTAGGCTTCGGAGGGGTCTACATACAAACAG
  ATGTGGGCGGGTCTGGGCTGTCACGTCTTGATACCTCTGTCATTTTTGAAGCCTTGGCTACAGGCTGCAC
  CAGCACCACAGCCTATATAAGCATCCACAACATGTGTGCCTGGATGATTGATAGCTTCGGAAATGAGGAA
  CAGAGGCACAAATTTTGCCCACCGCTCTGTACCATGGAGAAGTTTGCTTCCTACTGCCTCACTGAACCAG
  GAAGTGGGAGTGATGCTGCCTCTCTTCTGACCTCCGCTAAGAAACAGGGAGATCATTACATCCTCAATGG
  CTCCAAGGCCTTCATCAGTGGTGCTGGTGAGTCAGACATCTATGTGGTCATGTGCCGAACAGGAGGACTA
  GGCCCCAAGGGCATCTCATGCATAGTTGTTGAGAAGGGGACCCCTGGCCTCAGCTTTGGCAAGAAGGAGA
  AAAAGGTGGGGTGGAACTCCCAGCCAACACGAGCTGTGATCTTCGAAGACTGTGCTGTCCCTGTGGCCAA
  CAGAATTGGGAGCGAGGGGCAGGGCTTCCTCATTGCCGTGAGAGGACTGAACGGAGGGAGGATCAATATT
  GCTTCCTGCTCCCTGGGGGCTGCCCACGCCTCTGTCATCCTCACCCGAGACCACCTCAATGTCCGGAAGC
  AGTTTGGAGAGCCTCTGGCCAGTAACCAGTACTTGCAATTCACACTGGCTGATATGGCAACAAGGCTGGT
  GGCCGCGCGGCTGATGGTCCGCAATGCAGCAGTGGCTCTGCAGGAGGAGAGGAAGGATGCAGTGGCCTTG
  TGCTCCATGGCCAAGCTCTTTGCTACAGATGAATGCTTTGCCATCTGCAACCAGGCCTTGCAGATGCACG
  GGGGCTACGGCTACCTGAAGGATTACGCTGTTCAGCAGTACGTGCGGGACTCCAGGGTCCACCAGATTCT
  AGAAGGTAGCAATGAAGTGATGAGGATACTGATCTCTAGAAGCCTGCTTCAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201507 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLWSGCRRFGARLGCLPGGLRVLVQTGHRSLTSCIDPSMGLNEEQKEFQKVAFDFAAREMAPNMAEWDQK
  ELFPVDVMRKAAQLGFGGVYIQTDVGGSGLSRLDTSVIFEALATGCTSTTAYISIHNMCAWMIDSFGNEE
  QRHKFCPPLCTMEKFASYCLTEPGSGSDAASLLTSAKKQGDHYILNGSKAFISGAGESDIYVVMCRTGGL
  GPKGISCIVVEKGTPGLSFGKKEKKVGWNSQPTRAVIFEDCAVPVANRIGSEGQGFLIAVRGLNGGRINI
  ASCSLGAAHASVILTRDHLNVRKQFGEPLASNQYLQFTLADMATRLVAARLMVRNAAVALQEERKDAVAL
  CSMAKLFATDECFAICNQALQMHGGYGYLKDYAVQQYVRDSRVHQILEGSNEVMRILISRSLLQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014384
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014384.1, NM_014384.2, NP_055199.1
• RefSeq Size: 2216 bp
• RefSeq ORF: 1248 bp
• Locus ID: 27034
• Domains: Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• MW: 45.1 kDa
• Gene Summary: This gene encodes a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The encoded protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency. [provided by RefSeq, Nov 2009]