ACAD8 (NM_014384) Human Tagged ORF Clone

CAT#: RG201507

ACAD8 (GFP-tagged) - Human acyl-CoA dehydrogenase family, member 8 (ACAD8), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ACAD8
• Synonyms: ACAD-8; ARC42
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201507 representing NM_014384
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGAGCGGCTGCCGGCGTTTCGGGGCGCGCCTCGGCTGCCTGCCCGGCGGTCTCCGGGTCCTCG
  TCCAGACCGGCCACCGGAGCTTGACCTCCTGCATCGACCCTTCCATGGGACTTAATGAAGAGCAGAAAGA
  ATTTCAAAAAGTGGCCTTTGACTTTGCTGCCCGAGAGATGGCTCCAAATATGGCAGAGTGGGACCAGAAG
  GAGCTGTTCCCAGTGGATGTGATGCGGAAGGCAGCCCAGCTAGGCTTCGGAGGGGTCTACATACAAACAG
  ATGTGGGCGGGTCTGGGCTGTCACGTCTTGATACCTCTGTCATTTTTGAAGCCTTGGCTACAGGCTGCAC
  CAGCACCACAGCCTATATAAGCATCCACAACATGTGTGCCTGGATGATTGATAGCTTCGGAAATGAGGAA
  CAGAGGCACAAATTTTGCCCACCGCTCTGTACCATGGAGAAGTTTGCTTCCTACTGCCTCACTGAACCAG
  GAAGTGGGAGTGATGCTGCCTCTCTTCTGACCTCCGCTAAGAAACAGGGAGATCATTACATCCTCAATGG
  CTCCAAGGCCTTCATCAGTGGTGCTGGTGAGTCAGACATCTATGTGGTCATGTGCCGAACAGGAGGACTA
  GGCCCCAAGGGCATCTCATGCATAGTTGTTGAGAAGGGGACCCCTGGCCTCAGCTTTGGCAAGAAGGAGA
  AAAAGGTGGGGTGGAACTCCCAGCCAACACGAGCTGTGATCTTCGAAGACTGTGCTGTCCCTGTGGCCAA
  CAGAATTGGGAGCGAGGGGCAGGGCTTCCTCATTGCCGTGAGAGGACTGAACGGAGGGAGGATCAATATT
  GCTTCCTGCTCCCTGGGGGCTGCCCACGCCTCTGTCATCCTCACCCGAGACCACCTCAATGTCCGGAAGC
  AGTTTGGAGAGCCTCTGGCCAGTAACCAGTACTTGCAATTCACACTGGCTGATATGGCAACAAGGCTGGT
  GGCCGCGCGGCTGATGGTCCGCAATGCAGCAGTGGCTCTGCAGGAGGAGAGGAAGGATGCAGTGGCCTTG
  TGCTCCATGGCCAAGCTCTTTGCTACAGATGAATGCTTTGCCATCTGCAACCAGGCCTTGCAGATGCACG
  GGGGCTACGGCTACCTGAAGGATTACGCTGTTCAGCAGTACGTGCGGGACTCCAGGGTCCACCAGATTCT
  AGAAGGTAGCAATGAAGTGATGAGGATACTGATCTCTAGAAGCCTGCTTCAGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201507 representing NM_014384
  Red=Cloning site Green=Tags(s)
  
  MLWSGCRRFGARLGCLPGGLRVLVQTGHRSLTSCIDPSMGLNEEQKEFQKVAFDFAAREMAPNMAEWDQK
  ELFPVDVMRKAAQLGFGGVYIQTDVGGSGLSRLDTSVIFEALATGCTSTTAYISIHNMCAWMIDSFGNEE
  QRHKFCPPLCTMEKFASYCLTEPGSGSDAASLLTSAKKQGDHYILNGSKAFISGAGESDIYVVMCRTGGL
  GPKGISCIVVEKGTPGLSFGKKEKKVGWNSQPTRAVIFEDCAVPVANRIGSEGQGFLIAVRGLNGGRINI
  ASCSLGAAHASVILTRDHLNVRKQFGEPLASNQYLQFTLADMATRLVAARLMVRNAAVALQEERKDAVAL
  CSMAKLFATDECFAICNQALQMHGGYGYLKDYAVQQYVRDSRVHQILEGSNEVMRILISRSLLQE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014384
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014384.2, NP_055199.1
• RefSeq Size: 2216
• RefSeq ORF: 1248
• Locus ID: 27034
• Domains: Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The encoded protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency. [provided by RefSeq, Nov 2009]