PRP18 homolog (PRPF18) (NM_003675) Human Tagged ORF Clone

CAT#: RC201556

PRPF18 (Myc-DDK-tagged)-Human PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae) (PRPF18)

Product Images

Western blot validation of overexpression lysate (Cat# LY418505) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201556 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PRPF18 protein (Cat# TP301556). The protein was produced from HEK293T cells transfected with PRPF18 cDNA clone (Cat# RC201556) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRPF18
• Synonyms: hPrp18; PRP18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201556 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATTCTGAAATCAGAGATCCTTCGGAAGCGGCAGCTGGTGGAGGACAGGAACCTGCTGGTGGAAA
  ATAAAAAATATTTCAAGCGTAGTGAGCTCGCCAAAAAAGAAGAGGAAGCATATTTTGAAAGATGTGGCTA
  CAAGATACAGCCAAAAGAGGAGGACCAGAAACCATTAACTTCATCGAATCCAGTGTTAGAACTTGAACTG
  GCAGAGGAAAAATTACCTATGACGCTTTCTAGGCAAGAGGTCATCAGAAGATTGAGAGAAAGAGGAGAAC
  CAATCAGACTATTTGGAGAGACTGATTATGATGCTTTTCAACGTTTAAGGAAAATAGAGATCCTCACACC
  AGAAGTTAACAAGGGATTGAGGAATGATTTGAAAGCAGCCTTGGATAAGATTGATCAGCAGTACCTCAAT
  GAAATCGTCGGCGGTCAGGAGCCTGGAGAGGAAGACACACAGAATGATCTGAAAGTTCATGAGGAAAACA
  CCACAATTGAAGAGTTAGAGGCGCTTGGAGAGTCCTTAGGGAAAGGCGATGATCATAAGGACATGGACAT
  CATCACCAAATTCCTGAAGTTTCTTCTTGGCGTTTGGGCTAAAGAATTGAATGCCAGAGAAGATTATGTG
  AAACGCAGTGTGCAGGGTAAACTGAACAGTGCGACCCAGAAACAGACCGAGTCCTACCTAAGACCACTTT
  TTAGAAAGCTACGGAAAAGGAATCTTCCTGCTGATATTAAAGAATCAATAACGGATATTATTAAATTCAT
  GTTGCAGAGAGAATACGTGAAGGCAAATGATGCTTATCTTCAGATGGCCATTGGAAATGCGCCTTGGCCC
  ATCGGTGTCACTATGGTTGGTATCCATGCCAGAACTGGCAGAGAAAAGATTTTTTCCAAGCATGTTGCAC
  ATGTTTTAAATGACGAAACTCAGCGGAAATATATTCAGGGATTGAAGAGGTTAATGACCATTTGCCAGAA
  ACACTTTCCTACAGACCCATCCAAATGTGTGGAGTACAATGCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201556 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDILKSEILRKRQLVEDRNLLVENKKYFKRSELAKKEEEAYFERCGYKIQPKEEDQKPLTSSNPVLELEL
  AEEKLPMTLSRQEVIRRLRERGEPIRLFGETDYDAFQRLRKIEILTPEVNKGLRNDLKAALDKIDQQYLN
  EIVGGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDDHKDMDIITKFLKFLLGVWAKELNAREDYV
  KRSVQGKLNSATQKQTESYLRPLFRKLRKRNLPADIKESITDIIKFMLQREYVKANDAYLQMAIGNAPWP
  IGVTMVGIHARTGREKIFSKHVAHVLNDETQRKYIQGLKRLMTICQKHFPTDPSKCVEYNAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003675
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003675.1, NM_003675.2, NM_003675.3, NP_003666.1
• RefSeq Size: 1711 bp
• RefSeq ORF: 1029 bp
• Locus ID: 8559
• Domains: SFM, Prp18
• Protein Pathways: Spliceosome
• MW: 39.9 kDa
• Gene Summary: Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008]