UBOX5 (NM_014948) Human Tagged ORF Clone

CAT#: RC201812

UBOX5 (Myc-DDK-tagged)-Human U-box domain containing 5 (UBOX5), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414914) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201812 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified UBOX5 protein (Cat# TP301812). The protein was produced from HEK293T cells transfected with UBOX5 cDNA clone (Cat# RC201812) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBOX5
• Synonyms: hUIP5; RNF37; UBCE7IP5; UIP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201812 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCAGCTGATGGTT
  ACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGTTTCAGGACAGAGTATTTCAT
  TAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAATGTGGAAATCTGTAGGATCAACATAGACCTC
  ACAGCTGGGGGAGGTCAGAACGTCACTGGCCTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTT
  GGAATACGCCCCAGTGCCGGACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTT
  GGTAGGCAAAGTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
  CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGGAATAAAGGGG
  CTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTGACAGGCGGCGGTATCCCTTG
  TATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACCTGCTCCCAGGAAGTGATAGACAGCATCCTG
  CTGGTCACCTCAGAGAACCTGCCTCAGGATGTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACT
  GTGACCCTGGGGACCAGCCTGAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCA
  GGATGTGCCTGAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
  GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGCCGAGTGCCCA
  GTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCTCACCCCTCCCTCAAGGCCCG
  GATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGCCACCTGCTTGGGAGAGCACAGACGGCATTG
  GCAGTGATCCCTTCTTCCATTGTTCTGCCCTCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAG
  ACAGTAACTTTGGTGTAAATGCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGA
  GCACACTGCTAAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
  CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGCTCTATGCCCT
  CCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGAGGGAGCAACACTTCCTGGAG
  GCCTGGCACCGGCTCGGAGCAGCCTGGGAGCATCCTGGGCCCCGAATGTGCCTCCTGCAAAAGAGTATTT
  TCTCCCTACTTCAAAAAGGAGCCGGTGTACCAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGG
  GTGAGAAGCAACGCTCCCTGCCCATGACGTGCACAGCCTGCCAGCGGCCGGTTGCTAGCCAAGACGTGCT
  GCGGGTCCACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201812 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVINLCLPQFRPRIHCNKISADGYEVENLISEDLTKRSHGFRTEYFIKPPVYVTVSFPFNVEICRINIDL
  TAGGGQNVTGLEMYTSASSSRVSWNTPQCRTLGPAEPSVPDKEAFTLVGKVLLKNQSQVVFSHRGFKARP
  PFGAMEATLPSPAVVAQELWNKGALSLSHVAHLRICITHVTGGGIPCIKRLEVWGQPAKTCSQEVIDSIL
  LVTSENLPQDVALQAPALPMESDCDPGDQPESQQAPSSLQKLAEIIQDVPEEFLDPITLEIMPCPMLLPS
  GKVIDQSTLEKCNRSEATWGRVPSDPFTGVAFTPHSQPLPHPSLKARIDHFLLQHSIPGCHLLGRAQTAL
  AVIPSSIVLPSQKRKIEQAEHVPDSNFGVNASCFSATSPLVLPTTSEHTAKKMKATNEPSLTHMDCSTGP
  LSHEQKLSQSLEIALASTLGSMPSFTARLTRGQLQHLGTRGSNTSWRPGTGSEQPGSILGPECASCKRVF
  SPYFKKEPVYQLPCGHLLCRPCLGEKQRSLPMTCTACQRPVASQDVLRVHF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014948
• ORF Size: 1623 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014948.1, NM_014948.2, NM_014948.3, NP_055763.1
• RefSeq Size: 4362 bp
• RefSeq ORF: 1626 bp
• Locus ID: 22888
• Domains: U-box
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 59 kDa
• Gene Summary: This gene encodes a U-box domain containing protein. The encoded protein interacts with E2 enzymes and may play a role in the ubiquitination pathway. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]