AP4 (REPIN1) (NM_014374) Human Tagged ORF Clone

CAT#: RC201848

REPIN1 (Myc-DDK-tagged)-Human replication initiator 1 (REPIN1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY420504) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224200 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: REPIN1
• Synonyms: AP4; RIP60; Zfp464; ZNF464
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201848 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCTGGCCCAGCCCCGACTCCTTTCTGGGCCCTCCCAGGAGTCACCCCAGACCCTGGGGAAGGAGT
  CCCGCGGGCTGAGGCAACAAGGCACGTCAGTGGCCCAGTCTGGTGCCCAAGCCCCAGGCAGGGCCCATCG
  CTGTGCCCACTGTCGAAGGCACTTCCCTGGCTGGGTGGCTCTGTGGCTTCACACCCGCCGGTGCCAGGCC
  CGGCTGCCCTTGCCCTGCCCTGAGTGTGGCCGTCGCTTTCGCCATGCCCCCTTCTTAGCACTGCACCGCC
  AGGTCCATGCTGCTGCCACCCCAGACCTGGGCTTTGCCTGCCACCTCTGTGGGCAGAGCTTCCGAGGCTG
  GGTGGCCCTGGTTCTGCATCTGCGGGCCCATTCAGCTGCAAAGCGGCCCATCGCTTGTCCCAAATGCGAG
  AGACGCTTCTGGCGACGAAAGCAGCTTCGAGCTCATCTGCGGCGGTGCCACCCTCCCGCCCCGGAGGCCC
  GGCCCTTCATATGCGGCAACTGTGGCCGGAGCTTTGCCCAGTGGGACCAGCTAGTTGCCCACAAGCGGGT
  GCACGTAGCTGAGGCCCTGGAGGAGGCCGCAGCCAAGGCTCTGGGGCCCCGGCCCAGGGGCCGCCCCGCG
  GTGACCGCCCCCCGGCCCGGTGGAGATGCCGTCGACCGCCCCTTCCAGTGTGCCTGTTGTGGCAAGCGCT
  TCCGGCACAAGCCCAACTTGATCGCTCACCGCCGCGTGCACACGGGCGAGCGGCCCCACCAGTGCCCCGA
  GTGCGGGAAGCGCTTTACCAATAAGCCCTATCTGACTTCGCACCGGCGCATCCACACCGGCGAGAAGCCC
  TACCCGTGCAAAGAGTGCGGCCGCCGCTTCCGGCACAAACCCAACCTGCTGTCTCACAGCAAGATTCACA
  AGCGATCCGAGGGGTCGGCCCAGGCCGCCCCCGGCCCGGGGAGCCCCCAGCTGCCAGCCGGCCCCCAGGA
  GTCCGCGGCCGAGCCCACCCCGGCGGTACCTCTGAAACCGGCCCAGGAGCCGCCGCCAGGGGCCCCGCCA
  GAGCACCCGCAGGACCCGATCGAAGCCCCCCCCTCCCTCTACAGCTGCGACGACTGCGGCAGGAGCTTCC
  GGCTGGAGCGCTTCCTGCGGGCCCACCAGCGGCAGCACACCGGGGAGCGGCCCTTCACCTGCGCCGAGTG
  CGGGAAGAACTTCGGCAAGAAGACGCACCTGGTGGCGCACTCGCGCGTGCACTCCGGCGAGCGGCCCTTC
  GCCTGCGAGGAGTGCGGCCGCCGCTTCTCCCAGGGCAGCCATCTGGCGGCGCATCGGCGCGACCACGCCC
  CCGATCGGCCCTTCGTGTGTCCCGACTGCGGCAAGGCCTTCCGCCACAAACCCTACCTGGCGGCGCACCG
  GCGCATCCACACCGGCGAGAAGCCCTACGTCTGCCCCGACTGCGGCAAAGCCTTCAGCCAGAAGTCCAAC
  CTGGTGTCGCACCGGCGCATCCACACGGGCGAGCGGCCCTACGCCTGTCCCGACTGCGACCGCAGCTTCA
  GCCAGAAGTCCAACCTCATCACCCACCGCAAGAGCCACATCCGGGACGGCGCCTTCTGCTGTGCCATCTG
  TGGCCAGACCTTCGACGACGAGGAGAGACTCCTGGCCCACCAGAAGAAGCACGATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201848 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLAQPRLLSGPSQESPQTLGKESRGLRQQGTSVAQSGAQAPGRAHRCAHCRRHFPGWVALWLHTRRCQA
  RLPLPCPECGRRFRHAPFLALHRQVHAAATPDLGFACHLCGQSFRGWVALVLHLRAHSAAKRPIACPKCE
  RRFWRRKQLRAHLRRCHPPAPEARPFICGNCGRSFAQWDQLVAHKRVHVAEALEEAAAKALGPRPRGRPA
  VTAPRPGGDAVDRPFQCACCGKRFRHKPNLIAHRRVHTGERPHQCPECGKRFTNKPYLTSHRRIHTGEKP
  YPCKECGRRFRHKPNLLSHSKIHKRSEGSAQAAPGPGSPQLPAGPQESAAEPTPAVPLKPAQEPPPGAPP
  EHPQDPIEAPPSLYSCDDCGRSFRLERFLRAHQRQHTGERPFTCAECGKNFGKKTHLVAHSRVHSGERPF
  ACEECGRRFSQGSHLAAHRRDHAPDRPFVCPDCGKAFRHKPYLAAHRRIHTGEKPYVCPDCGKAFSQKSN
  LVSHRRIHTGERPYACPDCDRSFSQKSNLITHRKSHIRDGAFCCAICGQTFDDEERLLAHQKKHDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014374
• ORF Size: 1668 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014374.1, NM_014374.2, NP_055189.1
• RefSeq Size: 2894 bp
• RefSeq ORF: 1704 bp
• Locus ID: 29803
• Domains: zf-C2H2
• MW: 62.3 kDa
• Gene Summary: Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending. [UniProtKB/Swiss-Prot Function]