ABHD5 (NM_016006) Human Tagged ORF Clone

CAT#: RC201869

ABHD5 (Myc-DDK-tagged)-Human abhydrolase domain containing 5 (ABHD5)

Product Images

Western blot validation of overexpression lysate (Cat# LY402485) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201869 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ABHD5 protein (Cat# TP301869). The protein was produced from HEK293T cells transfected with ABHD5 cDNA clone (Cat# RC201869) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABHD5
• Synonyms: CGI58; IECN2; NCIE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201869 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGAGGAGGAGGAGGTGGACTCTGCCGACACCGGAGAGAGGTCAGGATGGCTAACTGGTTGGC
  TCCCCACATGGTGCCCTACGTCTATATCACACCTTAAAGAAGCTGAAGAGAAGATGTTAAAATGTGTGCC
  TTGCACATACAAAAAAGAACCTGTTCGTATATCTAATGGAAATAAAATATGGACACTGAAGTTCTCTCAT
  AATATTTCAAATAAGACTCCACTTGTCCTTCTCCATGGTTTTGGAGGAGGTCTTGGGCTCTGGGCACTGA
  ATTTTGGAGATCTTTGCACCAACAGACCTGTCTATGCTTTTGACCTATTGGGTTTTGGACGAAGTAGTAG
  ACCCAGGTTTGACAGTGATGCAGAAGAAGTGGAGAATCAGTTTGTGGAATCCATTGAAGAGTGGAGATGT
  GCCCTAGGATTGGACAAAATGATCTTGCTTGGGCACAACCTAGGTGGATTCTTGGCTGCTGCTTACTCGC
  TGAAGTACCCATCAAGGGTTAATCATCTCATTTTAGTGGAGCCTTGGGGTTTCCCTGAACGACCAGACCT
  TGCTGATCAAGACAGACCAATTCCAGTTTGGATCAGAGCCTTGGGAGCAGCATTGACTCCCTTTAACCCT
  TTAGCTGGCCTAAGGATTGCAGGACCCTTTGGTTTAAGTCTAGTGCAGCGTTTAAGGCCTGATTTCAAAC
  GAAAGTATTCTTCAATGTTCGAAGACGATACTGTGACAGAATACATCTACCACTGTAATGTGCAGACTCC
  AAGTGGTGAGACAGCTTTCAAGAATATGACTATTCCTTATGGATGGGCAAAAAGGCCAATGCTCCAGCGA
  ATTGGTAAAATGCACCCTGACATTCCAGTTTCAGTGATCTTTGGCGCCCGATCCTGCATAGATGGCAATT
  CTGGCACCAGCATCCAGTCCTTACGACCACATTCATATGTGAAGACAATAGCTATTCTTGGGGCAGGACA
  TTATGTATATGCAGATCAACCAGAAGAATTCAACCAGAAAGTAAAGGAGATCTGCGACACTGTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201869 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAEEEEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSH
  NISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRC
  ALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNP
  LAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPMLQR
  IGKMHPDIPVSVIFGARSCIDGNSGTSIQSLRPHSYVKTIAILGAGHYVYADQPEEFNQKVKEICDTVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016006
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016006.1, NM_016006.2, NM_016006.3, NM_016006.4, NP_057090.2
• RefSeq Size: 5370 bp
• RefSeq ORF: 1050 bp
• Locus ID: 51099
• Domains: abhydrolase
• Protein Families: Protease
• MW: 39.1 kDa
• Gene Summary: The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq, Jul 2008]