TRIM29 (NM_012101) Human Tagged ORF Clone

CAT#: RC201916

TRIM29 (Myc-DDK-tagged)-Human tripartite motif containing 29 (TRIM29)

Product Images

Coomassie blue staining of purified TRIM29 protein (Cat# TP301916). The protein was produced from HEK293T cells transfected with TRIM29 cDNA clone (Cat# RC201916) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM29
• Synonyms: ATDC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201916 representing NM_012101
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCTGCAGATGCCTCCAGGAGCAACGGGTCGAGCCCAGAAGCCAGGGATGCCCGGAGCCCGTCGG
  GCCCCAGTGGCAGCCTGGAGAATGGCACCAAGGCTGACGGCAAGGATGCCAAGACCACCAACGGGCACGG
  CGGGGAGGCAGCTGAGGGCAAGAGCCTGGGCAGCGCCCTGAAGCCAGGGGAAGGTAGGAGCGCCCTGTTC
  GCGGGCAATGAGTGGCGGCGACCCATCATCCAGTTTGTCGAGTCCGGGGACGACAAGAACTCCAACTACT
  TCAGCATGGACTCTATGGAAGGCAAGAGGTCGCCGTACGCAGGGCTCCAGCTGGGGGCTGCCAAGAAGCC
  ACCCGTTACCTTTGCCGAAAAGGGCGAGCTGCGCAAGTCCATTTTCTCGGAGTCCCGGAAGCCCACGGTG
  TCCATCATGGAGCCCGGGGAGACCCGGCGGAACAGCTACCCCCGGGCCGACACGGGCCTTTTTTCACGGT
  CCAAGTCCGGCTCCGAGGAGGTGCTGTGCGACTCCTGCATCGGCAACAAGCAGAAGGCGGTCAAGTCCTG
  CCTGGTGTGCCAGGCCTCCTTCTGCGAGCTGCATCTCAAGCCCCACCTGGAGGGCGCCGCCTTCCGAGAC
  CACCAGCTGCTCGAGCCCATCCGGGACTTTGAGGCCCGCAAGTGTCCCGTGCATGGCAAGACGATGGAGC
  TCTTCTGCCAGACCGACCAGACCTGCATCTGCTACCTTTGCATGTTCCAGGAGCACAAGAATCATAGCAC
  CGTGACAGTGGAGGAGGCCAAGGCCGAGAAGGAGACGGAGCTGTCACTGCAAAAGGAGCAGCTGCAGCTC
  AAGATCATTGAGATTGAGGATGAAGCTGAGAAGTGGCAGAAGGAGAAGGACCGCATCAAGAGCTTCACCA
  CCAATGAGAAGGCCATCCTGGAGCAGAACTTCCGGGACCTGGTGCGGGACCTGGAGAAGCAAAAGGAGGA
  AGTGAGGGCTGCGCTGGAGCAGCGGGAGCAGGATGCTGTGGACCAAGTGAAGGTGATCATGGATGCTCTG
  GATGAGAGAGCCAAGGTGCTGCATGAGGACAAGCAGACCCGGGAGCAGCTGCATAGCATCAGCGACTCTG
  TGTTGTTTCTGCAGGAATTTGGTGCATTGATGAGCAATTACTCTCTCCCCCCACCCCTGCCCACCTATCA
  TGTCCTGCTGGAGGGGGAGGGCCTGGGACAGTCACTAGGCAACTTCAAGGACGACCTGCTCAATGTATGC
  ATGCGCCACGTTGAGAAGATGTGCAAGGCGGACCTGAGCCGTAACTTCATTGAGAGGAACCACATGGAGA
  ACGGTGGTGACCATCGCTATGTGAACAACTACACGAACAGCTTCGGGGGTGAGTGGAGTGCACCGGACAC
  CATGAAGAGATACTCCATGTACCTGACACCCAAAGGTGGGGTCCGGACATCATACCAGCCCTCGTCTCCT
  GGCCGCTTCACCAAGGAGACCACCCAGAAGAATTTCAACAATCTCTATGGCACCAAAGGTAACTACACCT
  CCCGGGTCTGGGAGTACTCCTCCAGCATTCAGAACTCTGACAATGACCTGCCCGTCGTCCAAGGCAGCTC
  CTCCTTCTCCCTGAAAGGCTATCCCTCCCTCATGCGGAGCCAAAGCCCCAAGGCCCAGCCCCAGACTTGG
  AAATCTGGCAAGCAGACTATGCTGTCTCACTACCGGCCATTCTACGTCAACAAAGGCAACGGGATTGGGT
  CCAACGAAGCCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201916 representing NM_012101
  Red=Cloning site Green=Tags(s)
  
  MEAADASRSNGSSPEARDARSPSGPSGSLENGTKADGKDAKTTNGHGGEAAEGKSLGSALKPGEGRSALF
  AGNEWRRPIIQFVESGDDKNSNYFSMDSMEGKRSPYAGLQLGAAKKPPVTFAEKGELRKSIFSESRKPTV
  SIMEPGETRRNSYPRADTGLFSRSKSGSEEVLCDSCIGNKQKAVKSCLVCQASFCELHLKPHLEGAAFRD
  HQLLEPIRDFEARKCPVHGKTMELFCQTDQTCICYLCMFQEHKNHSTVTVEEAKAEKETELSLQKEQLQL
  KIIEIEDEAEKWQKEKDRIKSFTTNEKAILEQNFRDLVRDLEKQKEEVRAALEQREQDAVDQVKVIMDAL
  DERAKVLHEDKQTREQLHSISDSVLFLQEFGALMSNYSLPPPLPTYHVLLEGEGLGQSLGNFKDDLLNVC
  MRHVEKMCKADLSRNFIERNHMENGGDHRYVNNYTNSFGGEWSAPDTMKRYSMYLTPKGGVRTSYQPSSP
  GRFTKETTQKNFNNLYGTKGNYTSRVWEYSSSIQNSDNDLPVVQGSSSFSLKGYPSLMRSQSPKAQPQTW
  KSGKQTMLSHYRPFYVNKGNGIGSNEAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012101
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012101.1, NM_012101.2, NM_012101.3, NP_036233.2
• RefSeq Size: 3037 bp
• RefSeq ORF: 1767 bp
• Locus ID: 23650
• Protein Families: Transcription Factors
• MW: 65.8 kDa
• Gene Summary: The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype. [provided by RefSeq, Jul 2008]