PCCB (NM_000532) Human Tagged ORF Clone

CAT#: RC202019

PCCB (Myc-DDK-tagged)-Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCCB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202019 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCATTACGGGTGGCGGCGGTCGGGGCAAGGCTCAGCGTTCTGGCGAGCGGTCTCCGCGCCG
  CGGTCCGCAGCCTTTGCAGCCAGGCCACCTCTGTTAACGAACGCATCGAAAACAAGCGCCGGACCGCGCT
  GCTGGGAGGGGGCCAACGCCGTATTGACGCGCAGCACAAGCGAGGAAAGCTAACAGCCAGGGAGAGGATC
  AGTCTCTTGCTGGACCCTGGCAGCTTTGTTGAGAGCGACATGTTTGTGGAACACAGATGTGCAGATTTTG
  GAATGGCTGCTGATAAGAATAAGTTTCCTGGAGACAGCGTGGTCACTGGACGAGGCCGAATCAATGGAAG
  ATTGGTTTATGTCTTCAGTCAGGATTTTACAGTTTTTGGAGGCAGTCTGTCAGGAGCACATGCCCAAAAG
  ATCTGCAAAATCATGGACCAGGCCATAACGGTGGGGGCTCCAGTGATTGGGCTGAATGACTCTGGGGGAG
  CACGGATCCAAGAAGGAGTGGAGTCTTTGGCTGGCTATGCAGACATCTTTCTGAGGAATGTTACGGCATC
  CGGAGTCATCCCTCAGATTTCTCTGATCATGGGCCCATGTGCTGGTGGGGCCGTCTACTCCCCAGCCCTA
  ACAGACTTCACGTTCATGGTAAAGGACACCTCCTACCTGTTCATCACTGGCCCTGATGTTGTGAAGTCTG
  TCACCAATGAGGATGTTACCCAGGAGGAGCTCGGTGGTGCCAAGACCCACACCACCATGTCAGGTGTGGC
  CCACAGAGCTTTTGAAAATGATGTTGATGCCTTGTGTAATCTCCGGGATTTCTTCAACTACCTGCCCCTG
  AGCAGTCAGGACCCGGCTCCCGTCCGTGAGTGCCACGATCCCAGTGACCGTCTGGTTCCTGAGCTTGACA
  CAATTGTCCCTTTGGAATCAACCAAAGCCTACAACATGGTGGACATCATACACTCTGTTGTTGATGAGCG
  TGAATTTTTTGAGATCATGCCCAATTATGCCAAGAACATCATTGTTGGTTTTGCAAGAATGAATGGGAGG
  ACTGTTGGAATTGTTGGCAACCAACCTAAGGTGGCCTCAGGATGCTTGGATATTAATTCATCTGTGAAAG
  GGGCTCGTTTTGTCAGATTCTGTGATGCATTCAATATTCCACTCATCACTTTTGTTGATGTCCCTGGCTT
  TCTACCTGGCACAGCACAGGAATACGGGGGCATCATCCGGCATGGTGCCAAGCTTCTCTACGCATTTGCT
  GAGGCAACTGTACCCAAAGTCACAGTCATCACCAGGAAGGCCTATGGAGGTGCCTATGATGTCATGAGCT
  CTAAGCACCTTTGTGGTGATACCAACTATGCCTGGCCCACCGCAGAGATTGCAGTCATGGGAGCAAAGGG
  CGCTGTGGAGATCATCTTCAAAGGGCATGAGAATGTGGAAGCTGCTCAGGCAGAGTACATCGAGAAGTTT
  GCCAACCCTTTCCCTGCAGCAGTGCGAGGGTTTGTGGATGACATCATCCAACCTTCTTCCACACGTGCCC
  GAATCTGCTGTGACCTGGATGTCTTGGCCAGCAAGAAGGTACAACGTCCTTGGAGAAAACATGCAAATAT
  TCCATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202019 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAALRVAAVGARLSVLASGLRAAVRSLCSQATSVNERIENKRRTALLGGGQRRIDAQHKRGKLTARERI
  SLLLDPGSFVESDMFVEHRCADFGMAADKNKFPGDSVVTGRGRINGRLVYVFSQDFTVFGGSLSGAHAQK
  ICKIMDQAITVGAPVIGLNDSGGARIQEGVESLAGYADIFLRNVTASGVIPQISLIMGPCAGGAVYSPAL
  TDFTFMVKDTSYLFITGPDVVKSVTNEDVTQEELGGAKTHTTMSGVAHRAFENDVDALCNLRDFFNYLPL
  SSQDPAPVRECHDPSDRLVPELDTIVPLESTKAYNMVDIIHSVVDEREFFEIMPNYAKNIIVGFARMNGR
  TVGIVGNQPKVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLYAFA
  EATVPKVTVITRKAYGGAYDVMSSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKF
  ANPFPAAVRGFVDDIIQPSSTRARICCDLDVLASKKVQRPWRKHANIPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000532
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000532.1, NM_000532.2, NM_000532.3, NM_000532.4, NP_000523.1, NP_000523.2
• RefSeq Size: 1825 bp
• RefSeq ORF: 1620 bp
• Locus ID: 5096
• Cytogenetics: 3q22.3
• Domains: Carboxyl_trans
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• MW: 58.2 kDa
• Gene Summary: 'The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]'