PCCB (NM_000532) Human Untagged Clone

CAT#: SC119822

PCCB (untagged)-Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCCB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119822 sequence for NM_000532 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGCATTACGGGTGGCGGCGGTCGGGGCAAGGCTCAGCGTTCTGGCGAGCGGT
  CTCCGCGCCGCGGTCCGCAGCCTTTGCAGCCAGGCCACCTCTGTTAACGAACGCATCGAA
  AACAAGCGCCGGACCGCGCTGCTGGGAGGGGGCCAACGCCGTATTGACGCGCAGCACAAG
  CGAGGAAAGCTAACAGCCAGGGAGAGGATCAGTCTCTTGCTGGACCCTGGCAGCTTTGTT
  GAGAGCGACATGTTTGTGGAACACAGATGTGCAGATTTTGGAATGGCTGCTGATAAGAAT
  AAGTTTCCTGGAGACAGCGTGGTCACTGGACGAGGCCGAATCAATGGAAGATTGGTTTAT
  GTCTTCAGTCAGGATTTTACAGTTTTTGGAGGCAGTCTGTCAGGAGCACATGCCCAAAAG
  ATCTGCAAAATCATGGACCAGGCCATAACGGTGGGGGCTCCAGTGATTGGGCTGAATGAC
  TCTGGGGGAGCACGGATCCAAGAAGGAGTGGAGTCTTTGGCTGGCTATGCAGACATCTTT
  CTGAGGAATGTTACGGCATCCGGAGTCATCCCTCAGATTTCTCTGATCATGGGCCCATGT
  GCTGGTGGGGCCGTCTACTCCCCAGCCCTAACAGACTTCACGTTCATGGTAAAGGACACC
  TCCTACCTGTTCATCACTGGCCCTGATGTTGTGAAGTCTGTCACCAATGAGGATGTTACC
  CAGGAGGAGCTCGGTGGTGCCAAGACCCACACCACCATGTCAGGTGTGGCCCACAGAGCT
  TTTGAAAATGATGTTGATGCCTTGTGTAATCTCCGGGATTTCTTCAACTACCTGCCCCTG
  AGCAGTCAGGACCCGGCTCCCGTCCGTGAGTGCCACGATCCCAGTGACCGTCTGGTTCCT
  GAGCTTGACACAATTGTCCCTTTGGAATCAACCAAAGCCTACAACATGGTGGACATCATA
  CACTCTGTTGTTGATGAGCGTGAATTTTTTGAGATCATGCCCAATTATGCCAAGAACATC
  ATTGTTGGTTTTGCAAGAATGAATGGGAGGACTGTTGGAATTGTTGGCAACCAACCTAAG
  GTGGCCTCAGGATGCTTGGATATTAATTCATCTGTGAAAGGGGCTCGTTTTGTCAGATTC
  TGTGATGCATTCAATATTCCACTCATCACTTTTGTTGATGTCCCTGGCTTTCTACCTGGC
  ACAGCACAGGAATACGGGGGCATCATCCGGCATGGTGCCAAGCTTCTCTACGCATTTGCT
  GAGGCAACTGTACCCAAAGTCACAGTCATCACCAGGAAGGCCTATGGAGGTGCCTATGAT
  GTCATGAGCTCTAAGCACCTTTGTGGTGATACCAACTATGCCTGGCCCACCGCAGAGATT
  GCAGTCATGGGAGCAAAGGGCGCTGTGGAGATCATCTTCAAAGGGCATGAGAATGTGGAA
  GCTGCTCAGGCAGAGTACATCGAGAAGTTTGCCAACCCTTTCCCTGCAGCAGTGCGAGGG
  TTTGTGGATGACATCATCCAACCTTCTTCCACACGTGCCCGAATCTGCTGTGACCTGGAT
  GTCTTGGCCAGCAAGAAGGTACAACGTCCTTGGAGAAAACATGCAAATATTCCATTGTAA
  
  Clone variation with respect to NM_000532.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000532 unedited
  GGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCCTCGTGCC
  GAATTCGGCACGAGGGCCGGCACAGCAAAAAGGCGGCGGCATTACGGGTGGCGGCGGTCG
  GGGCAAGGCTCAGCGTTCTGGCGAGCGGTCTCCGCGCCGCGGTCCGCAGCCTTTGCAGCC
  AGGCCACCTCTGTTAACGAACGCATCGAAAACAAGCGCCGGACCGCGCTGCTGGGAGGGG
  GCCAACGCCGTATTGACGCGCAGCACAAGCGAGGAAAGCTAACAGCCAGGGAGAGGATCA
  GTCTCTTGCTGGACCCTGGCAGCTTTGTTGAGAGCGACATGTTTGTGGAACACAGATGTG
  CAGATTTTGGAATGGCTGCTGATAAGAATAAGTTTCCTGGAGACAGCGTGGTCACTGGAC
  GAGGCCGAATCAATGGAAGATTGGTTTATGTCTTCAGTCAGGATTTTACAGTTTTTGGAG
  GCAGTCTGTCAGGAGCACATGCCCAAAAGATCTGCAAAATCATGGACCAGGCCATAACGG
  TGGGGGCTCCAGTGATTGGGCTGAATGACTCTGGGGGAGCACGGATCCAAGAAGGAGTGG
  AGTCTTTGGCTGGCTATGCAGACATCTTTCTGAGGAATGTTACGGCATCCGGAGTCATCC
  CTCAGATTTCTCTGATCATGGGCCCATGTGCTGGTGGGGCCGTCTACTCCCCAGCCCTAA
  CAGACTTCACGTTCATGGTAAAGGACACCTCCTACCTGTTCATCACTGGCCCTGATGTTG
  TGAAGTCTGTCACCATGGAAGATGTTACCCAGGAAGAGCCTGGGTGGTGCCAAGACCCCA
  CCACCATGTCAGGTGTGCCCACAGAAGCTTTGAAAAAGAAGGTGAAGCCTTGTGTAACTC
  TCGGGATTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000532 unedited
  GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACTATTTTTCTTTCTAGTTGTA
  TTGATTTTTCAGATTTCCACCTAAAAAAATCGAATTTATGCTCAAAAAATACAACTTAAA
  ATGTTTAAAAAAGTAAATAATTTTACAGTAAGGCACAAAAGAGATCTTTCTAGAATTTAA
  TAAACTTAGTTATTCTAAGTTATCCAACTATTTGGATTCCCAGGTTTCATGATTGCAAAA
  GGCAGGAATGGGATGTGAATGGGCAGACAGTAATTCAGTTCTTGGTTTCTTTTCCTTTGA
  TTTGTTTACAATGGAATATTTGCATGTTTTCTCCAAGGACGTTGTACCTTCTTGCTGGCC
  AAGACATCCAGGTCACAGCAGATTCGGGCACGTGTGGAAGAAGGTTGGATGATGTCATCC
  ACAAACCCTCGCACTGCTGCAGGGAAAGGGTTGGCAAACTTCTCGATGTACTCTGCCTGA
  GCAGCTTCCACATTCTCATGCCCTTTGAAGATGATCTCCACAGCGCCCTTTGCTCCCATG
  ACTGCAATCTCTGCGGTGGGCCAGGCATAGTTGGTATCACCACAAAGGTGCTTAGAGCTC
  ATGACATCATAGGCACCTCCATAGGCCTTCCTGGTGATGACTGTGACTTTGGGTACAGTT
  GCCTCAGCAAATGCGTAGAGAAGCTTGGCACCATGCCGGATGATGCCCCCGTATTCCTGT
  GCTGTGCCAGGTAGAAAGCCAGGGACATCAACAAAAGTGATGAGTGGGATATTGAATGCA
  TCACAGAATCTGACAAAACGAGCCCCTTTCACAGATGAAATATATTCAGCATCCTGAGGG
  CCACTTAGGTTGGTTGCCACAATNNCACAGTNNCTCCATCATTCTTGCAAAACAACATGA
  TGGTTTGGGCATATGGGCATGATCTACAAAATCACGCTATAACACACGAGGATGATGTCA
  CCTGTTGNAGCCTTGGTGATCCCAAGAC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000532
• Insert Size: 1960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000532.2, NP_000523.1
• RefSeq Size: 1829 bp
• RefSeq ORF: 1620 bp
• Locus ID: 5096
• Cytogenetics: 3q22.3
• Domains: Carboxyl_trans
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: 'The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]'
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1).