SLU7 (NM_006425) Human Tagged ORF Clone

CAT#: RC202113

SLU7 (Myc-DDK-tagged)-Human SLU7 splicing factor homolog (S. cerevisiae) (SLU7)

Product Images

Western blot validation of overexpression lysate (Cat# LY416650) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202113 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SLU7 protein (Cat# TP302113). The protein was produced from HEK293T cells transfected with SLU7 cDNA clone (Cat# RC202113) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLU7
• Synonyms: 9G8; hSlu7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202113 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCCACAGTTGTAGATGCAGTTAATGCTGCACCCCTATCGGGGTCCAAAGAAATGAGTTTGGAAG
  AACCAAAGAAGATGACCAGAGAGGACTGGAGAAAGAAGAAGGAGCTAGAAGAACAGCGAAAATTGGGCAA
  TGCTCCTGCAGAAGTTGATGAAGAAGGAAAAGACATCAACCCCCATATTCCTCAGTATATTTCTTCAGTG
  CCATGGTATATTGATCCTTCAAAAAGACCTACTTTAAAACACCAGAGACCACAACCAGAAAAACAAAAGC
  AGTTCAGCTCATCTGGAGAATGGTACAAGAGGGGTGTAAAAGAGAATTCCGTAATTACTAAGTACCGCAA
  AGGAGCATGTGAAAATTGTGGGGCCATGACACACAAAAAGAAAGACTGCTTTGAGAGACCTAGGCGAGTT
  GGAGCCAAATTTACAGGTACTAATATAGCTCCAGATGAACATGTCCAGCCTCAACTGATGTTTGACTATG
  ATGGGAAGAGGGATCGGTGGAATGGCTACAATCCAGAAGAACACATGAAAATTGTTGAAGAGTATGCCAA
  AGTTGATTTGGCAAAACGAACATTGAAAGCCCAGAAACTCCAAGAGGAATTAGCCTCAGGAAAATTAGTG
  GAACAGGCTAATTCTCCAAAACACCAGTGGGGAGAAGAGGAACCAAATTCTCAGACGGAAAAAGATCATA
  ATAGTGAAGATGAGGATGAAGATAAATATGCAGATGATATTGACATGCCTGGACAGAATTTTGACTCCAA
  GAGACGAATTACTGTCCGGAATCTCAGGATTCGAGAAGATATTGCAAAATATTTGAGGAATTTAGATCCA
  AATTCTGCCTACTATGATCCAAAAACTAGAGCAATGAGAGAGAATCCTTATGCCAATGCAGGAAAGAATC
  CAGATGAAGTGAGTTATGCTGGAGATAACTTTGTTAGGTACACAGGAGATACCATTTCAATGGCTCAGAC
  ACAGTTGTTTGCATGGGAAGCCTATGACAAGGGATCTGAAGTGCATCTACAGGCAGATCCTACAAAGCTA
  GAGCTGTTGTATAAGTCCTTCAAAGTCAAAAAAGAAGATTTCAAAGAACAGCAGAAAGAAAGCATCCTGG
  AAAAGTATGGTGGCCAAGAACATTTGGATGCCCCTCCAGCTGAATTGCTTTTAGCCCAGACTGAAGACTA
  TGTGGAGTACTCAAGACATGGGACAGTCATCAAAGGACAGGAGCGGGCTGTTGCCTGCTCTAAGTATGAG
  GAGGATGTGAAGATCCACAATCACACACATATCTGGGGATCGTACTGGAAAGAAGGCCGATGGGGATACA
  AATGCTGTCACTCTTTTTTCAAGTATTCCTATTGTACTGGAGAAGCTGGGAAGGAGATTGTTAACTCTGA
  GGAGTGTATTATAAATGAGATAACTGGGGAAGAATCTGTGAAAAAACCTCAAACCCTCATGGAGCTGCAT
  CAAGAAAAACTGAAAGAGGAAAAGAAGAAGAAGAAAAAGAAAAAGAAGAAGCATCGAAAGAGCAGTTCAG
  ATAGTGATGATGAAGAAAAGAAGCATGAAAAATTGAAAAAGGCACTGAACGCAGAGGAGGCCCGCCTTCT
  TCATGTCAAGGAGACCATGCAGATTGATGAGAGGAAGCGGCCTTACAATAGCATGTATGAAACTCGAGAA
  CCTACTGAAGAGGAAATGGAGGCATATAGAATGAAACGTCAGAGGCCAGATGACCCCATGGCCTCTTTCC
  TTGGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202113 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSATVVDAVNAAPLSGSKEMSLEEPKKMTREDWRKKKELEEQRKLGNAPAEVDEEGKDINPHIPQYISSV
  PWYIDPSKRPTLKHQRPQPEKQKQFSSSGEWYKRGVKENSVITKYRKGACENCGAMTHKKKDCFERPRRV
  GAKFTGTNIAPDEHVQPQLMFDYDGKRDRWNGYNPEEHMKIVEEYAKVDLAKRTLKAQKLQEELASGKLV
  EQANSPKHQWGEEEPNSQTEKDHNSEDEDEDKYADDIDMPGQNFDSKRRITVRNLRIREDIAKYLRNLDP
  NSAYYDPKTRAMRENPYANAGKNPDEVSYAGDNFVRYTGDTISMAQTQLFAWEAYDKGSEVHLQADPTKL
  ELLYKSFKVKKEDFKEQQKESILEKYGGQEHLDAPPAELLLAQTEDYVEYSRHGTVIKGQERAVACSKYE
  EDVKIHNHTHIWGSYWKEGRWGYKCCHSFFKYSYCTGEAGKEIVNSEECIINEITGEESVKKPQTLMELH
  QEKLKEEKKKKKKKKKKHRKSSSDSDDEEKKHEKLKKALNAEEARLLHVKETMQIDERKRPYNSMYETRE
  PTEEEMEAYRMKRQRPDDPMASFLGQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006425
• ORF Size: 1758 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006425.1, NM_006425.2, NM_006425.3, NM_006425.4, NP_006416.3
• RefSeq Size: 3570 bp
• RefSeq ORF: 1761 bp
• Locus ID: 10569
• Domains: zf-CCHC
• Protein Pathways: Spliceosome
• MW: 68.3 kDa
• Gene Summary: Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq, Jul 2008]