AlaRS (AARS) (NM_001605) Human Tagged ORF Clone

CAT#: RC202136

AARS (Myc-DDK-tagged)-Human alanyl-tRNA synthetase (AARS)

Product Images

Western blot validation of overexpression lysate (Cat# LY400604) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202136 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified AARS protein (Cat# TP302136). The protein was produced from HEK293T cells transfected with AARS cDNA clone (Cat# RC202136) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AARS
• Synonyms: CMT2N; EIEE29
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202136 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCTACTCTAACAGCAAGTGAAATCCGGCAGCGATTTATAGATTTCTTCAAGAGGAACGAGCATA
  CGTATGTTCACTCGTCTGCCACCATCCCATTGGATGACCCCACTTTGCTCTTTGCCAATGCAGGCATGAA
  CCAGTTTAAACCCATTTTCCTGAACACAATTGACCCATCTCACCCCATGGCAAAGCTGAGCAGAGCTGCC
  AATACCCAGAAGTGCATCCGGGCTGGGGGCAAACATAATGACCTGGACGATGTGGGCAAGGATGTCTATC
  ATCACACCTTCTTCGAGATGCTGGGCTCTTGGTCTTTTGGAGATTACTTTAAGGAATTGGCATGTAAGAT
  GGCTCTGGAACTCCTCACCCAAGAGTTTGGCATTCCCATTGAAAGACTTTATGTTACTTACTTTGGCGGG
  GATGAAGCAGCTGGCTTAGAAGCAGATCTGGAATGCAAACAGATCTGGCAAAATTTGGGGCTGGATGACA
  CCAAAATCCTCCCAGGCAACATGAAGGATAACTTCTGGGAGATGGGTGACACGGGCCCCTGTGGTCCTTG
  CAGTGAGATCCACTACGACCGGATTGGTGGTCGGGACGCCGCACATCTTGTCAACCAGGACGACCCTAAT
  GTGCTGGAGATCTGGAACCTTGTGTTCATCCAGTATAACAGGGAAGCTGATGGCATTCTGAAACCTCTTC
  CCAAGAAAAGCATTGACACAGGGATGGGCCTGGAACGACTGGTATCTGTGCTGCAGAATAAGATGTCCAA
  CTATGACACTGACCTTTTTGTCCCTTACTTTGAAGCCATTCAGAAGGGCACAGGTGCCCGACCATACACT
  GGGAAAGTTGGTGCTGAGGATGCCGATGGGATTGACATGGCCTACCGGGTGCTGGCTGACCATGCTCGGA
  CCATCACTGTGGCACTGGCTGATGGTGGCCGGCCTGACAACACAGGGCGTGGATATGTGTTGAGACGGAT
  TCTCCGCCGAGCTGTCCGATACGCCCATGAAAAGCTCAATGCCAGCAGGGGCTTCTTTGCTACGTTAGTG
  GATGTTGTCGTCCAGTCCCTGGGAGATGCATTTCCTGAGCTGAAGAAGGACCCAGACATGGTGAAGGACA
  TCATTAATGAAGAAGAGGTGCAGTTTCTCAAGACTCTCAGCAGAGGGCGTCGCATCCTGGACAGGAAAAT
  TCAGAGCCTGGGAGACAGCAAGACCATTCCCGGAGACACTGCTTGGCTCCTCTATGACACCTATGGGTTT
  CCAGTGGATCTGACTGGACTGATTGCTGAAGAGAAGGGCCTGGTGGTAGACATGGATGGCTTTGAAGAGG
  AGAGGAAACTGGCCCAGCTGAAATCACAGGGCAAGGGAGCTGGTGGGGAAGACCTCATTATGCTGGACAT
  TTACGCTATCGAAGAGCTCCGGGCACGGGGTCTGGAGGTCACAGATGATTCCCCAAAGTACAATTACCAT
  TTGGACTCCAGTGGTAGCTATGTATTTGAGAACACAGTGGCTACGGTGATGGCTCTGCGCAGGGAGAAGA
  TGTTCGTGGAAGAGGTGTCCACAGGCCAGGAGTGTGGAGTGGTGCTGGACAAGACCTGTTTCTATGCTGA
  GCAAGGAGGCCAGATCTATGACGAAGGCTACCTGGTGAAGGTGGATGACAGCAGTGAAGATAAAACAGAG
  TTTACAGTGAAGAATGCTCAGGTCCGAGGAGGGTATGTGCTACACATTGGAACCATCTACGGTGACCTGA
  AAGTGGGGGATCAGGTCTGGCTGTTTATTGATGAGCCCCGACGAAGACCCATCATGAGCAACCACACAGC
  TACGCACATTCTGAACTTCGCCCTGCGCTCAGTGCTTGGGGAAGCTGACCAGAAAGGCTCATTGGTTGCT
  CCTGACCGCCTCAGATTTGACTTTACTGCCAAGGGAGCCATGTCCACCCAACAGATCAAGAAGGCTGAAG
  AGATTGCTAATGAGATGATTGAGGCAGCCAAGGCCGTCTATACCCAGGATTGCCCCCTGGCAGCAGCGAA
  AGCCATCCAGGGCCTACGGGCTGTGTTTGATGAGACCTATCCTGACCCTGTGCGAGTCGTCTCCATTGGG
  GTCCCGGTGTCCGAGTTGCTGGATGACCCCTCTGGGCCTGCTGGCTCCCTGACTTCTGTTGAGTTCTGTG
  GGGGAACGCACCTGCGGAACTCGAGTCATGCAGGAGCTTTTGTGATCGTGACGGAAGAAGCCATTGCCAA
  GGGTATCCGGAGGATTGTGGCTGTCACAGGTGCCGAGGCCCAGAAGGCCCTCAGGAAAGCAGAGAGCTTG
  AAGAAATGTCTCTCTGTCATGGAAGCCAAAGTGAAGGCTCAGACTGCTCCAAACAAGGATGTGCAGAGGG
  AGATCGCTGACCTTGGAGAGGCCCTGGCCACTGCAGTCATCCCCCAGTGGCAGAAGGATGAATTGCGGGA
  GACTCTCAAATCCCTAAAGAAGGTCATGGATGACTTGGACCGAGCCAGCAAAGCCGATGTCCAGAAACGA
  GTGTTAGAGAAGACGAAGCAGTTCATCGACAGCAACCCCAACCAGCCTCTTGTCATCCTGGAGATGGAGA
  GCGGCGCCTCAGCCAAGGCCCTGAATGAAGCCTTGAAGCTCTTCAAGATGCACTCCCCTCAGACTTCTGC
  CATGCTCTTCACGGTGGACAATGAGGCTGGCAAGATCACGTGCCTGTGTCAAGTCCCCCAGAATGCAGCC
  AATCGGGGCTTAAAAGCCAGCGAGTGGGTGCAGCAGGTGTCAGGCTTGATGGACGGTAAAGGTGGTGGCA
  AGGATGTGTCTGCACAGGCCACAGGCAAGAACGTTGGCTGCCTGCAGGAGGCGCTGCAGCTGGCCACTTC
  CTTCGCCCAGCTGCGCCTCGGGGATGTAAAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202136 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAA
  NTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGG
  DEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPN
  VLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYT
  GKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLV
  DVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDSKTIPGDTAWLLYDTYGF
  PVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRARGLEVTDDSPKYNYH
  LDSSGSYVFENTVATVMALRREKMFVEEVSTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTE
  FTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVLGEADQKGSLVA
  PDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKAVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIG
  VPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESL
  KKCLSVMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKR
  VLEKTKQFIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQTSAMLFTVDNEAGKITCLCQVPQNAA
  NRGLKASEWVQQVSGLMDGKGGGKDVSAQATGKNVGCLQEALQLATSFAQLRLGDVKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001605
• ORF Size: 2904 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001605.1, NM_001605.2, NP_001596.1, NP_001596.2
• RefSeq Size: 3344 bp
• RefSeq ORF: 2907 bp
• Locus ID: 16
• Cytogenetics: 16q22.1
• Domains: tRNA-synt_2c, DHHA1
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 106.8 kDa
• Gene Summary: 'The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008]'