ACTR3B (NM_020445) Human Tagged ORF Clone
CAT#: RC202148
ACTR3B (Myc-DDK-tagged)-Human ARP3 actin-related protein 3 homolog B (yeast) (ACTR3B), transcript variant 1
"NM_020445" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ACTR3B |
Synonyms | ARP3BETA; ARP11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202148 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGCTCCCTGCCTCCCTGCGTGGTGGACTGTGGCACCGGGTATACCAAGCTTGGCTACGCAGGCA ACACTGAGCCCCAGTTCATTATTCCTTCATGTATTGCCATCAGAGAGTCAGCAAAGGTAGTTGACCAAGC TCAAAGGAGAGTGTTGAGGGGAGTTGATGACCTTGACTTTTTCATAGGAGATGAAGCCATCGATAAACCT ACATATGCTACAAAGTGGCCGATACGACATGGAATCATTGAAGACTGGGATCTTATGGAAAGGTTCATGG AGCAAGTGGTTTTTAAATATCTTCGAGCTGAACCTGAGGACCATTATTTTTTAATGACAGAACCTCCACT CAATACACCAGAAAACAGAGAGTATCTTGCAGAAATTATGTTTGAATCATTTAACGTACCAGGACTCTAC ATTGCAGTTCAGGCAGTGCTGGCCTTGGCGGCATCTTGGACATCTCGACAAGTGGGTGAACGTACGTTAA CGGGGATAGTCATTGACAGCGGAGATGGAGTCACCCATGTTATCCCAGTGGCAGAAGGTTATGTAATTGG AAGCTGCATCAAACACATCCCGATTGCAGGTAGAGATATTACGTATTTCATTCAACAGCTGCTAAGGGAG AGGGAGGTGGGAATCCCTCCTGAGCAGTCACTGGAGACCGCAAAAGCCATTAAGGAGAAATACTGTTACA TTTGCCCCGATATAGTCAAGGAATTTGCCAAGTATGATGTGGATCCCCGGAAGTGGATCAAACAGTACAC GGGTATCAATGCGATCAACCAGAAGAAGTTTGTTATAGACGTTGGTTACGAAAGATTCCTGGGACCTGAA ATATTCTTTCACCCGGAGTTTGCCAACCCAGACTTTATGGAGTCCATCTCAGATGTTGTTGATGAAGTAA TACAGAACTGCCCCATCGATGTGCGGCGCCCGCTGTATAAGAATGTCGTACTCTCAGGAGGCTCCACCAT GTTCAGGGATTTCGGACGCCGACTGCAGAGGGATTTGAAGAGAGTGGTGGATGCTAGGCTGAGGCTCAGC GAGGAGCTCAGCGGCGGGAGGATCAAGCCGAAGCCTGTGGAGGTCCAGGTGGTCACGCATCACATGCAGC GCTACGCCGTGTGGTTCGGAGGCTCCATGCTGGCCTCGACTCCCGAGTTCTTTCAGGTCTGCCACACCAA GAAGGACTATGAAGAGTACGGGCCCAGCATCTGCCGCCACAACCCCGTCTTTGGAGTCATGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202148 protein sequence
Red=Cloning site Green=Tags(s) MAGSLPPCVVDCGTGYTKLGYAGNTEPQFIIPSCIAIRESAKVVDQAQRRVLRGVDDLDFFIGDEAIDKP TYATKWPIRHGIIEDWDLMERFMEQVVFKYLRAEPEDHYFLMTEPPLNTPENREYLAEIMFESFNVPGLY IAVQAVLALAASWTSRQVGERTLTGIVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRE REVGIPPEQSLETAKAIKEKYCYICPDIVKEFAKYDVDPRKWIKQYTGINAINQKKFVIDVGYERFLGPE IFFHPEFANPDFMESISDVVDEVIQNCPIDVRRPLYKNVVLSGGSTMFRDFGRRLQRDLKRVVDARLRLS EELSGGRIKPKPVEVQVVTHHMQRYAVWFGGSMLASTPEFFQVCHTKKDYEEYGPSICRHNPVFGVMS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020445 |
ORF Size | 1254 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020445.1, NM_020445.2, NM_020445.3, NM_020445.4, NM_020445.5, NP_065178.1 |
RefSeq Size | 2223 bp |
RefSeq ORF | 1257 bp |
Locus ID | 57180 |
Domains | ACTIN |
MW | 47.6 kDa |
Gene Summary | This gene encodes a member of the actin-related proteins (ARP), which form multiprotein complexes and share 35-55% amino acid identity with conventional actin. The protein encoded by this gene may have a regulatory role in the actin cytoskeleton and induce cell-shape change and motility. Pseudogenes of this gene are located on chromosomes 2, 4, 10, 16, 22 and Y. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jul 2012] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC113083 | ACTR3B (untagged)-Human ARP3 actin-related protein 3 homolog B (yeast) (ACTR3B), transcript variant 1 |
USD 310.00 |
|
SC322271 | ACTR3B (untagged)-Human ARP3 actin-related protein 3 homolog B (yeast) (ACTR3B), transcript variant 1 |
USD 760.00 |
|
RG202148 | ACTR3B (GFP-tagged) - Human ARP3 actin-related protein 3 homolog B (yeast) (ACTR3B), transcript variant 1 |
USD 460.00 |
|
RC202148L3 | Lenti ORF clone of Human ARP3 actin-related protein 3 homolog B (yeast) (ACTR3B), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC202148L4 | Lenti ORF clone of Human ARP3 actin-related protein 3 homolog B (yeast) (ACTR3B), transcript variant 1, mGFP tagged |
USD 620.00 |
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