ACTR3B (NM_020445) Human Tagged ORF Clone

CAT#: RG202148

ACTR3B (GFP-tagged) - Human ARP3 actin-related protein 3 homolog B (yeast) (ACTR3B), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ACTR3B
• Synonyms: ARP3BETA; ARP11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202148 representing NM_020445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGCTCCCTGCCTCCCTGCGTGGTGGACTGTGGCACCGGGTATACCAAGCTTGGCTACGCAGGCA
  ACACTGAGCCCCAGTTCATTATTCCTTCATGTATTGCCATCAGAGAGTCAGCAAAGGTAGTTGACCAAGC
  TCAAAGGAGAGTGTTGAGGGGAGTTGATGACCTTGACTTTTTCATAGGAGATGAAGCCATCGATAAACCT
  ACATATGCTACAAAGTGGCCGATACGACATGGAATCATTGAAGACTGGGATCTTATGGAAAGGTTCATGG
  AGCAAGTGGTTTTTAAATATCTTCGAGCTGAACCTGAGGACCATTATTTTTTAATGACAGAACCTCCACT
  CAATACACCAGAAAACAGAGAGTATCTTGCAGAAATTATGTTTGAATCATTTAACGTACCAGGACTCTAC
  ATTGCAGTTCAGGCAGTGCTGGCCTTGGCGGCATCTTGGACATCTCGACAAGTGGGTGAACGTACGTTAA
  CGGGGATAGTCATTGACAGCGGAGATGGAGTCACCCATGTTATCCCAGTGGCAGAAGGTTATGTAATTGG
  AAGCTGCATCAAACACATCCCGATTGCAGGTAGAGATATTACGTATTTCATTCAACAGCTGCTAAGGGAG
  AGGGAGGTGGGAATCCCTCCTGAGCAGTCACTGGAGACCGCAAAAGCCATTAAGGAGAAATACTGTTACA
  TTTGCCCCGATATAGTCAAGGAATTTGCCAAGTATGATGTGGATCCCCGGAAGTGGATCAAACAGTACAC
  GGGTATCAATGCGATCAACCAGAAGAAGTTTGTTATAGACGTTGGTTACGAAAGATTCCTGGGACCTGAA
  ATATTCTTTCACCCGGAGTTTGCCAACCCAGACTTTATGGAGTCCATCTCAGATGTTGTTGATGAAGTAA
  TACAGAACTGCCCCATCGATGTGCGGCGCCCGCTGTATAAGAATGTCGTACTCTCAGGAGGCTCCACCAT
  GTTCAGGGATTTCGGACGCCGACTGCAGAGGGATTTGAAGAGAGTGGTGGATGCTAGGCTGAGGCTCAGC
  GAGGAGCTCAGCGGCGGGAGGATCAAGCCGAAGCCTGTGGAGGTCCAGGTGGTCACGCATCACATGCAGC
  GCTACGCCGTGTGGTTCGGAGGCTCCATGCTGGCCTCGACTCCCGAGTTCTTTCAGGTCTGCCACACCAA
  GAAGGACTATGAAGAGTACGGGCCCAGCATCTGCCGCCACAACCCCGTCTTTGGAGTCATGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202148 representing NM_020445
  Red=Cloning site Green=Tags(s)
  
  MAGSLPPCVVDCGTGYTKLGYAGNTEPQFIIPSCIAIRESAKVVDQAQRRVLRGVDDLDFFIGDEAIDKP
  TYATKWPIRHGIIEDWDLMERFMEQVVFKYLRAEPEDHYFLMTEPPLNTPENREYLAEIMFESFNVPGLY
  IAVQAVLALAASWTSRQVGERTLTGIVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRE
  REVGIPPEQSLETAKAIKEKYCYICPDIVKEFAKYDVDPRKWIKQYTGINAINQKKFVIDVGYERFLGPE
  IFFHPEFANPDFMESISDVVDEVIQNCPIDVRRPLYKNVVLSGGSTMFRDFGRRLQRDLKRVVDARLRLS
  EELSGGRIKPKPVEVQVVTHHMQRYAVWFGGSMLASTPEFFQVCHTKKDYEEYGPSICRHNPVFGVMS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020445
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020445.4, NP_065178.1
• RefSeq Size: 2199
• RefSeq ORF: 1257
• Locus ID: 57180
• Domains: ACTIN
• Gene Summary: This gene encodes a member of the actin-related proteins (ARP), which form multiprotein complexes and share 35-55% amino acid identity with conventional actin. The protein encoded by this gene may have a regulatory role in the actin cytoskeleton and induce cell-shape change and motility. Pseudogenes of this gene are located on chromosomes 2, 4, 10, 16, 22 and Y. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jul 2012]