VAT1 (NM_006373) Human Tagged ORF Clone

CAT#: RC202201

VAT1 (Myc-DDK-tagged)-Human vesicle amine transport protein 1 homolog (T. californica) (VAT1)

Product Images

Western blot validation of overexpression lysate (Cat# LY401915) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202201 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified VAT1 protein (Cat# TP302201). The protein was produced from HEK293T cells transfected with VAT1 cDNA clone (Cat# RC202201) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VAT1
• Synonyms: VATI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202201 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGACGAGAGAGAGGTAGCCGAGGCAGCGACCGGGGAAGACGCCTCTTCGCCGCCTCCGAAAACCG
  AGGCAGCGAGCGACCCCCAGCATCCCGCGGCCTCCGAAGGGGCCGCCGCCGCCGCCGCCTCGCCGCCACT
  GCTGCGCTGCCTAGTGCTCACCGGCTTTGGAGGCTACGACAAGGTGAAGCTGCAGAGCCGGCCGGCAGCG
  CCCCCGGCCCCTGGGCCCGGCCAGCTGACGCTGCGTCTGCGGGCCTGCGGGCTCAACTTCGCAGACCTCA
  TGGCTAGGCAGGGGCTGTACGACCGTCTCCCGCCTCTGCCTGTCACTCCGGGCATGGAGGGCGCGGGTGT
  TGTGATCGCAGTGGGCGAGGGAGTCAGCGACCGCAAGGCAGGAGACCGGGTGATGGTGTTGAACCGGTCA
  GGGATGTGGCAGGAAGAGGTGACTGTGCCCTCGGTCCAGACCTTCCTGATTCCTGAGGCCATGACCTTTG
  AGGAAGCTGCTGCCTTGCTCGTCAATTACATTACAGCCTACATGGTCCTCTTTGACTTCGGCAACCTACA
  GCCTGGCCACAGCGTCTTGGTACACATGGCTGCAGGGGGTGTGGGTATGGCTGCCGTGCAGCTGTGCCGT
  ACAGTGGAGAATGTGACAGTGTTCGGAACGGCCTCGGCCAGCAAGCACGAGGCACTGAAGGAGAATGGGG
  TCACACATCCCATCGACTATCACACGACTGACTACGTGGATGAGATCAAGAAGATTTCCCCTAAAGGAGT
  GGACATTGTCATGGACCCTCTGGGTGGGTCAGATACTGCCAAGGGCTACAACCTCCTGAAACCCATGGGC
  AAAGTCGTCACCTATGGAATGGCCAACCTGCTGACGGGCCCCAAACGGAACCTGATGGCCCTGGCCCGGA
  CATGGTGGAATCAGTTCAGCGTGACAGCTCTGCAGCTGCTGCAGGCCAACCGGGCTGTGTGTGGCTTCCA
  CCTGGGCTACCTGGATGGTGAGGTGGAGCTGGTCAGTGGTGTGGTGGCCCGCCTCCTGGCTCTGTACAAC
  CAGGGCCACATCAAGCCCCACATTGACTCAGTCTGGCCCTTCGAGAAGGTGGCTGATGCCATGAAACAGA
  TGCAGGAGAAGAAGAATGTGGGCAAGGTCCTCCTGGTTCCAGGGCCAGAGAAGGAGAAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202201 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAA
  PPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRS
  GMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCR
  TVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMG
  KVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYN
  QGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006373
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006373.2, NM_006373.3, NP_006364.2
• RefSeq Size: 2758 bp
• RefSeq ORF: 1182 bp
• Locus ID: 10493
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• MW: 41.9 kDa
• Gene Summary: Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins. [provided by RefSeq, Jul 2008]