VAT1 (NM_006373) Human Tagged ORF Clone
CAT#: RC202201
VAT1 (Myc-DDK-tagged)-Human vesicle amine transport protein 1 homolog (T. californica) (VAT1)
"NM_006373" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | VAT1 |
Synonyms | VATI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202201 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGACGAGAGAGAGGTAGCCGAGGCAGCGACCGGGGAAGACGCCTCTTCGCCGCCTCCGAAAACCG AGGCAGCGAGCGACCCCCAGCATCCCGCGGCCTCCGAAGGGGCCGCCGCCGCCGCCGCCTCGCCGCCACT GCTGCGCTGCCTAGTGCTCACCGGCTTTGGAGGCTACGACAAGGTGAAGCTGCAGAGCCGGCCGGCAGCG CCCCCGGCCCCTGGGCCCGGCCAGCTGACGCTGCGTCTGCGGGCCTGCGGGCTCAACTTCGCAGACCTCA TGGCTAGGCAGGGGCTGTACGACCGTCTCCCGCCTCTGCCTGTCACTCCGGGCATGGAGGGCGCGGGTGT TGTGATCGCAGTGGGCGAGGGAGTCAGCGACCGCAAGGCAGGAGACCGGGTGATGGTGTTGAACCGGTCA GGGATGTGGCAGGAAGAGGTGACTGTGCCCTCGGTCCAGACCTTCCTGATTCCTGAGGCCATGACCTTTG AGGAAGCTGCTGCCTTGCTCGTCAATTACATTACAGCCTACATGGTCCTCTTTGACTTCGGCAACCTACA GCCTGGCCACAGCGTCTTGGTACACATGGCTGCAGGGGGTGTGGGTATGGCTGCCGTGCAGCTGTGCCGT ACAGTGGAGAATGTGACAGTGTTCGGAACGGCCTCGGCCAGCAAGCACGAGGCACTGAAGGAGAATGGGG TCACACATCCCATCGACTATCACACGACTGACTACGTGGATGAGATCAAGAAGATTTCCCCTAAAGGAGT GGACATTGTCATGGACCCTCTGGGTGGGTCAGATACTGCCAAGGGCTACAACCTCCTGAAACCCATGGGC AAAGTCGTCACCTATGGAATGGCCAACCTGCTGACGGGCCCCAAACGGAACCTGATGGCCCTGGCCCGGA CATGGTGGAATCAGTTCAGCGTGACAGCTCTGCAGCTGCTGCAGGCCAACCGGGCTGTGTGTGGCTTCCA CCTGGGCTACCTGGATGGTGAGGTGGAGCTGGTCAGTGGTGTGGTGGCCCGCCTCCTGGCTCTGTACAAC CAGGGCCACATCAAGCCCCACATTGACTCAGTCTGGCCCTTCGAGAAGGTGGCTGATGCCATGAAACAGA TGCAGGAGAAGAAGAATGTGGGCAAGGTCCTCCTGGTTCCAGGGCCAGAGAAGGAGAAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC202201 protein sequence
Red=Cloning site Green=Tags(s) MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAA PPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRS GMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCR TVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMG KVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYN QGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006373 |
ORF Size | 1179 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006373.2, NM_006373.3, NP_006364.2 |
RefSeq Size | 2758 bp |
RefSeq ORF | 1182 bp |
Locus ID | 10493 |
Domains | ADH_zinc_N |
Protein Families | Druggable Genome |
MW | 41.9 kDa |
Gene Summary | Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116157 | VAT1 (untagged)-Human vesicle amine transport protein 1 homolog (T. californica) (VAT1) |
USD 760.00 |
|
SC322371 | VAT1 (untagged)-Human vesicle amine transport protein 1 homolog (T. californica) (VAT1) |
USD 760.00 |
|
RG202201 | VAT1 (GFP-tagged) - Human vesicle amine transport protein 1 homolog (T. californica) (VAT1) |
USD 460.00 |
|
RC202201L1 | Lenti ORF clone of Human vesicle amine transport protein 1 homolog (T. californica) (VAT1), Myc-DDK-tagged |
USD 768.00 |
|
RC202201L2 | Lenti ORF clone of Human vesicle amine transport protein 1 homolog (T. californica) (VAT1), mGFP tagged |
USD 620.00 |
|
RC202201L3 | Lenti ORF clone of Human vesicle amine transport protein 1 homolog (T. californica) (VAT1), Myc-DDK-tagged |
USD 620.00 |
|
RC202201L4 | Lenti ORF clone of Human vesicle amine transport protein 1 homolog (T. californica) (VAT1), mGFP tagged |
USD 620.00 |
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