SSH3 (NM_017857) Human Tagged ORF Clone

CAT#: RC202399

SSH3 (Myc-DDK-tagged)-Human slingshot homolog 3 (Drosophila) (SSH3)

Product Images

Western blot validation of overexpression lysate (Cat# LY413421) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202399 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SSH3 protein (Cat# TP302399). The protein was produced from HEK293T cells transfected with SSH3 cDNA clone (Cat# RC202399) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SSH3
• Synonyms: SSH3L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202399 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGGTCACAGTGAGCCGTTCGCCCCCGGGCAGCGGCGCCTCCACGCCCGTGGGGCCCTGGGACC
  AGGCGGTCCAGCGAAGGAGTCGACTCCAGCGAAGGCAGAGCTTTGCGGTGCTCCGTGGGGCTGTCCTGGG
  ACTGCAGGATGGAGGGGACAATGATGATGCAGCAGAGGCCAGTTCTGAGCCAACAGAGAAGGCCCCGAGT
  GAGGAGGAGCTCCACGGGGACCAGACAGACTTCGGGCAAGGATCCCAGAGTCCCCAGAAGCAGGAGGAGC
  AGAGGCAGCACCTGCACCTCATGGTACAGCTGCTGAGGCCGCAGGATGACATCCGCCTGGCAGCCCAGCT
  GGAGGCACCCCGGCCTCCCCGGCTCCGCTACCTGCTGGTAGTTTCTACACGAGAAGGAGAAGGTCTGAGC
  CAGGATGAGACGGTCCTCCTGGGCGTGGATTTCCCTGACAGCAGCTCCCCCAGCTGCACCCTGGGCCTGG
  TCTTGCCCCTCTGGAGTGACACCCAGGTGTACTTAGATGGAGACGGGGGCTTCAGCGTGACGTCTGGTGG
  GCAAAGCCGGATCTTCAAGCCCATCTCCATCCAGACCATGTGGGCCACACTCCAGGTATTGCACCAAGCA
  TGTGAGGCAGCTCTAGGCAGCGGCCTTGTACCGGGTGGCAGTGCCCTCACCTGGGCCAGCCACTACCAGG
  AGAGACTGAACTCCGAACAGAGCTGCCTCAATGAGTGGACGGCTATGGCCGACCTGGAGTCTCTGCGGCC
  TCCCAGCGCCGAGCCTGGCGGGTCCTCAGAACAGGAGCAGATGGAGCAGGCGATCCGTGCTGAGCTGTGG
  AAAGTGTTGGATGTCAGTGACCTGGAGAGTGTCACTTCCAAAGAGATCCGCCAGGCTCTGGAGCTGCGCC
  TGGGGCTCCCCCTCCAGCAGTACCGTGACTTCATCGACAACCAGATGCTGCTGCTGGTGGCACAGCGGGA
  CCGAGCCTCCCGCATCTTCCCCCACCTCTACCTGGGCTCAGAGTGGAACGCAGCAAACCTGGAGGAGCTG
  CAGAGGAACAGGGTCACCCACATCTTGAACATGGCCCGGGAGATTGACAACTTCTACCCTGAGCGCTTCA
  CCTACCACAATGTGCGCCTCTGGGATGAGGAGTCGGCCCAGCTGCTGCCGCACTGGAAGGAGACGCACCG
  CTTCATTGAGGCTGCAAGAGCACAGGGCACCCACGTGCTGGTCCACTGCAAGATGGGCGTCAGCCGCTCA
  GCGGCCACAGTGCTGGCCTATGCCATGAAGCAGTACGAATGCAGCCTGGAGCAGGCCCTGCGCCACGTGC
  AGGAGCTCCGGCCCATCGCCCGCCCCAACCCTGGCTTCCTGCGCCAGCTGCAGATCTACCAGGGCATCCT
  GACGGCCAGCCGCCAGAGCCATGTCTGGGAGCAGAAAGTGGGTGGGGTCTCCCCAGAGGAGCACCCAGCC
  CCTGAAGTCTCTACACCATTCCCACCTCTTCCGCCAGAACCTGAGGGTGGTGGGGAGGAGAAGGTTGTAG
  GCATGGAAGAGAGCCAGGCAGCCCCGAAAGAAGAGCCTGGGCCACGGCCACGTATAAACCTCCGAGGGGT
  CATGAGGTCCATCAGTCTTCTGGAGCCCTCCTTGGAGCTGGAGAGCACCTCAGAGACCAGTGACATGCCA
  GAGGTCTTCTCTTCCCACGAGTCTTCACATGAAGAGCCTCTGCAGCCCTTCCCACAGCTTGCAAGGACCA
  AGGGAGGCCAGCAGGTGGACAGGGGGCCTCAGCCTGCCCTGAAGTCCCGCCAGTCAGTGGTTACCCTCCA
  GGGCAGTGCCGTGGTGGCCAACCGGACCCAGGCCTTCCAGGAGCAGGAGCAGGGGCAGGGGCAGGGGCAG
  GGAGAGCCCTGCATTTCCTCTACGCCCAGGTTCCGGAAGGTGGTGAGACAGGCCAGCGTGCATGACAGTG
  GAGAGGAGGGCGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202399 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALVTVSRSPPGSGASTPVGPWDQAVQRRSRLQRRQSFAVLRGAVLGLQDGGDNDDAAEASSEPTEKAPS
  EEELHGDQTDFGQGSQSPQKQEEQRQHLHLMVQLLRPQDDIRLAAQLEAPRPPRLRYLLVVSTREGEGLS
  QDETVLLGVDFPDSSSPSCTLGLVLPLWSDTQVYLDGDGGFSVTSGGQSRIFKPISIQTMWATLQVLHQA
  CEAALGSGLVPGGSALTWASHYQERLNSEQSCLNEWTAMADLESLRPPSAEPGGSSEQEQMEQAIRAELW
  KVLDVSDLESVTSKEIRQALELRLGLPLQQYRDFIDNQMLLLVAQRDRASRIFPHLYLGSEWNAANLEEL
  QRNRVTHILNMAREIDNFYPERFTYHNVRLWDEESAQLLPHWKETHRFIEAARAQGTHVLVHCKMGVSRS
  AATVLAYAMKQYECSLEQALRHVQELRPIARPNPGFLRQLQIYQGILTASRQSHVWEQKVGGVSPEEHPA
  PEVSTPFPPLPPEPEGGGEEKVVGMEESQAAPKEEPGPRPRINLRGVMRSISLLEPSLELESTSETSDMP
  EVFSSHESSHEEPLQPFPQLARTKGGQQVDRGPQPALKSRQSVVTLQGSAVVANRTQAFQEQEQGQGQGQ
  GEPCISSTPRFRKVVRQASVHDSGEEGEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017857
• ORF Size: 1977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017857.2, NM_017857.3, NP_060327.2
• RefSeq Size: 2894 bp
• RefSeq ORF: 1980 bp
• Locus ID: 54961
• Domains: DSPc
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 73 kDa
• Gene Summary: The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]). [supplied by OMIM, Mar 2008]