VPS25 (NM_032353) Human Tagged ORF Clone

CAT#: RC202487

VPS25 (Myc-DDK-tagged)-Human vacuolar protein sorting 25 homolog (S. cerevisiae) (VPS25)

Product Images

Western blot validation of overexpression lysate (Cat# LY410190) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202487 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified VPS25 protein (Cat# TP302487). The protein was produced from HEK293T cells transfected with VPS25 cDNA clone (Cat# RC202487) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VPS25
• Synonyms: DERP9; EAP20; FAP20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202487 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATGAGTTTCGAGTGGCCGTGGCAGTATCGCTTCCCACCCTTCTTTACGTTACAACCGAATGTGG
  ACACTCGGCAGAAGCAGCTGGCCGCCTGGTGCTCGCTGGTCCTGTCCTTCTGCCGCCTGCACAAACAGTC
  CAGCATGACGGTGATGGAAGCTCAGGAGAGCCCGCTCTTCAACAACGTCAAGCTACAGCGAAAGCTTCCT
  GTGGAGTCGATCCAGATTGTATTAGAGGAACTGAGGAAGAAAGGGAACCTCGAGTGGTTGGATAAGAGCA
  AGTCCAGCTTCCTGATCATGTGGCGGAGGCCAGAAGAATGGGGGAAACTCATCTATCAGTGGGTTTCCAG
  GAGTGGCCAGAACAACTCCGTCTTTACCCTGTATGAACTGACTAATGGGGAAGACACAGAGGATGAGGAG
  TTCCACGGGCTGGATGAAGCCACTCTACTGCGGGCTCTGCAGGCCCTACAGCAGGAGCACAAGGCCGAGA
  TCATCACTGTCAGCGATGGCCGAGGCGTCAAGTTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202487 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLP
  VESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEE
  FHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032353
• ORF Size: 528 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032353.1, NM_032353.2, NM_032353.3, NP_115729.1
• RefSeq Size: 1120 bp
• RefSeq ORF: 531 bp
• Locus ID: 84313
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis
• MW: 20.7 kDa
• Gene Summary: This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]