VPS25 (NM_032353) Human Tagged ORF Clone

CAT#: RG202487

VPS25 (GFP-tagged) - Human vacuolar protein sorting 25 homolog (S. cerevisiae) (VPS25)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: VPS25
• Synonyms: DERP9; EAP20; FAP20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202487 representing NM_032353
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATGAGTTTCGAGTGGCCGTGGCAGTATCGCTTCCCACCCTTCTTTACGTTACAACCGAATGTGG
  ACACTCGGCAGAAGCAGCTGGCCGCCTGGTGCTCGCTGGTCCTGTCCTTCTGCCGCCTGCACAAACAGTC
  CAGCATGACGGTGATGGAAGCTCAGGAGAGCCCGCTCTTCAACAACGTCAAGCTACAGCGAAAGCTTCCT
  GTGGAGTCGATCCAGATTGTATTAGAGGAACTGAGGAAGAAAGGGAACCTCGAGTGGTTGGATAAGAGCA
  AGTCCAGCTTCCTGATCATGTGGCGGAGGCCAGAAGAATGGGGGAAACTCATCTATCAGTGGGTTTCCAG
  GAGTGGCCAGAACAACTCCGTCTTTACCCTGTATGAACTGACTAATGGGGAAGACACAGAGGATGAGGAG
  TTCCACGGGCTGGATGAAGCCACTCTACTGCGGGCTCTGCAGGCCCTACAGCAGGAGCACAAGGCCGAGA
  TCATCACTGTCAGCGATGGCCGAGGCGTCAAGTTCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202487 representing NM_032353
  Red=Cloning site Green=Tags(s)
  
  MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLP
  VESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEE
  FHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032353
• ORF Size: 528 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032353.2, NP_115729.1
• RefSeq Size: 1122
• RefSeq ORF: 531
• Locus ID: 84313
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]