Probable hydrolase PNKD (PNKD) (NM_022572) Human Tagged ORF Clone

CAT#: RC202515

PNKD (Myc-DDK-tagged)-Human paroxysmal nonkinesigenic dyskinesia (PNKD), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY411638) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202515 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNKD
• Synonyms: BRP17; DYT8; FKSG19; FPD1; KIPP1184; MR-1; MR-1S; MR1; PDC; PKND1; PNKD1; TAHCCP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202515 representing NM_022572
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGGCAGGGCTGGCCCGCGGCGTGGCAGTGGGTCGCCGGCTGCTGGCTCCTCCTCGTCCTTGTCC
  TCGTCCTACTTGTGAGCCCCCGCGGCTGCCGAGCGCGGCGGGGCCTCCGCGGTCTGCTCATGGCGCACAG
  CCAGCGGCTGCTCTTCCGAATCGGGTACAGCCTGTACACCCGCACCTGGCTCGGGTACCTCTTCTACCGA
  CAGCAGCTGCGCAGGGCTCGGAATCGCTACCCTAAAGGCCACTCGAAAACCCAGCCCCGCCTCTTCAATG
  GAGTGAAGGTGCTTCCCATCCCTGTCCTCTCGGACAACTACAGCTACCTCATCATCGACACCCAGGCCCA
  GCTGGCTGTGGCTGTGGACCCTTCAGACCCTCGGGCTGTGCAGGCTTCCATTGAAAAGGAAGGGGTCACC
  TTGGTCGCCATTCTGTGTACTCACAAGCACTGGGACCACAGTGGAGGGAACCGTGACCTCAGCCGGCGGC
  ACCGGGACTGTCGGGTGTACGGGAGCCCTCAGGACGGCATCCCCTACCTCACCCATCCCCTGTGTCATCA
  AGATGTGGTCAGCGTGGGACGGCTTCAGATCCGGGCCCTGGCTACACCTGGCCACACACAAGGCCATCTG
  GTCTACCTACTGGATGGGGAGCCCTACAAGGGTCCCTCCTGCCTCTTCTCAGGGGACCTGCTCTTCCTCT
  CTGGCTGTGGGCGGACCTTTGAGGGCAATGCAGAGACCATGCTGAGCTCACTGGACACTGTGCTGGGGCT
  AGGGGATGACACCCTTCTGTGGCCTGGTCATGAGTATGCAGAGGAGAACCTGGGCTTTGCAGGTGTGGTG
  GAGCCCGAGAACCTGGCCCGGGAGAGGAAGATGCAGTGGGTGCAGCGGCAGCGGCTGGAGCGCAAGGGCA
  CGTGCCCATCTACCCTGGGAGAGGAGCGCTCCTACAACCCGTTCCTGAGAACCCACTGCCTGGCGCTACA
  GGAGGCTCTGGGGCCGGGGCCGGGCCCCACTGGGGATGATGACTACTCCCGGGCCCAGCTCCTGGAAGAG
  CTCCGCCGGCTGAAGGATATGCACAAGAGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202515 representing NM_022572
  Red=Cloning site Green=Tags(s)
  
  MAWQGWPAAWQWVAGCWLLLVLVLVLLVSPRGCRARRGLRGLLMAHSQRLLFRIGYSLYTRTWLGYLFYR
  QQLRRARNRYPKGHSKTQPRLFNGVKVLPIPVLSDNYSYLIIDTQAQLAVAVDPSDPRAVQASIEKEGVT
  LVAILCTHKHWDHSGGNRDLSRRHRDCRVYGSPQDGIPYLTHPLCHQDVVSVGRLQIRALATPGHTQGHL
  VYLLDGEPYKGPSCLFSGDLLFLSGCGRTFEGNAETMLSSLDTVLGLGDDTLLWPGHEYAEENLGFAGVV
  EPENLARERKMQWVQRQRLERKGTCPSTLGEERSYNPFLRTHCLALQEALGPGPGPTGDDDYSRAQLLEE
  LRRLKDMHKSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022572
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022572.1, NM_022572.2, NM_022572.3, NM_022572.4, NP_072094.1
• RefSeq Size: 2986
• RefSeq ORF: 1086
• Locus ID: 25953
• Domains: lactamase_B
• Protein Families: Transmembrane
• MW: 40.6 kDa
• Gene Summary: This gene is thought to play a role in the regulation of myofibrillogenesis. Mutations in this gene have been associated with the movement disorder paroxysmal non-kinesigenic dyskinesia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]