EPB41L1 (NM_177996) Human Tagged ORF Clone

CAT#: RC202596

EPB41L1 (Myc-DDK-tagged)-Human erythrocyte membrane protein band 4.1-like 1 (EPB41L1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY403596) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202596 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EPB41L1 protein (Cat# TP302596). The protein was produced from HEK293T cells transfected with EPB41L1 cDNA clone (Cat# RC202596) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EPB41L1
• Synonyms: 4.1N; MRD11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202596 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGAAGGACTACAGTGAGGCCGATGGCCTTTCGGAGAGGACCACGCCCAGCAAGGCCCAGAAAT
  CGCCCCAGAAGATTGCCAAGAAATACAAGAGTGCCATCTGCCGGGTCACTCTGCTTGATGCCTCGGAGTA
  TGAGTGTGAGGTGGAGAAACATGGCCGGGGCCAGGTGCTGTTTGACCTGGTCTGTGAACACCTCAACCTC
  CTAGAGAAGGACTACTTCGGCCTGACCTTCTGTGATGCTGACAGCCAGAAGAACTGGCTGGACCCCTCCA
  AGGAGATCAAGAAGCAGATCCGGAGTAGCCCCTGGAATTTTGCCTTCACAGTCAAGTTCTACCCGCCTGA
  TCCTGCCCAGCTGACAGAAGACATCACAAGATACTACCTGTGCCTGCAGCTGCGGGCAGACATCATCACG
  GGCCGGCTGCCATGCTCCTTTGTCACGCATGCCCTACTGGGCTCCTACGCTGTGCAGGCTGAGCTGGGTG
  ACTATGATGCTGAGGAGCATGTGGGCAACTATGTCAGCGAGCTCCGCTTCGCCCCTAACCAGACCCGGGA
  GCTGGAGGAGAGGATCATGGAGCTGCATAAGACATATAGGGGGATGACCCCGGGAGAAGCAGAAATCCAC
  TTCTTAGAGAATGCCAAGAAGCTTTCCATGTACGGAGTAGACCTGCACCATGCCAAGGACTCTGAGGGCA
  TCGACATCATGTTAGGCGTTTGTGCCAATGGCCTGCTCATCTACCGGGACCGGCTGAGAATCAACCGCTT
  TGCCTGGCCCAAGATCCTCAAGATCTCCTACAAGAGGAGTAACTTCTATATCAAGATCCGGCCTGGGGAG
  TATGAGCAATTTGAGAGCACAATTGGCTTTAAGCTCCCAAACCACCGGTCAGCCAAGAGACTGTGGAAGG
  TCTGCATCGAGCATCATACATTCTTCCGGCTGGTGTCCCCTGAGCCCCCACCCAAGGGCTTCCTGGTGAT
  GGGCTCCAAGTTCCGGTACAGTGGGAGGACCCAGGCACAGACTCGCCAGGCCAGCGCCCTCATTGACCGG
  CCTGCACCCTTCTTTGAGCGTTCTTCCAGCAAACGGTACACCATGTCCCGCAGCCTTGATGGAGCAGAGT
  TCTCCCGCCCAGCCTCGGTCAGCGAGAACCATGATGCAGGGCCTGACGGTGACAAGCGGGATGAGGATGG
  CGAGTCTGGGGGGCAACGGTCAGAGGCTGAGGAGGGAGAGGTCAGGACTCCAACCAAGATCAAGGAGCTA
  AAGTTCTTAGACAAGCCAGAAGATGTCTTGCTGAAGCACCAGGCCAGCATCAATGAGCTCAAAAGGACCC
  TGAAGGAGCCCAACAGCAAACTCATCCACCGGGATCGAGACTGGGAACGGGAGCGCAGGCTGCCCTCCTC
  CCCCGCCTCCCCCTCCCCCAAGGGCACCCCTGAGAAAGCCAATGAGAGAGCAGGGCTGAGGGAGGGCTCC
  GAGGAGAAAGTCAAACCACCACGTCCCCGGGCCCCAGAGAGTGACACAGGCGATGAGGACCAGGACCAGG
  AGAGGGACACGGTGTTCCTGAAGGACAACCACCTGGCCATTGAGCGCAAGTGCTCCAGCATCACGGTCAG
  CTCTACGTCTAGCCTGGAGGCTGAGGTGGACTTCACGGTCATTGGTGACTACCATGGCAGCGCCTTCGAA
  GACTTCTCCCGCAGCCTGCCTGAGCTCGACCGGGACAAAAGCGACTCGGACACTGAGGGCCTGCTGTTCT
  CCCGGGATCTCAACAAGGGGGCCCCCAGCCAGGATGATGAGTCTGGGGGCATTGAGGACAGCCCGGATCG
  AGGGGCCTGCTCCACCCCGGATATGCCCCAGTTTGAGCCCGTGAAAACAGAAACCATGACTGTCAGCAGT
  CTGGCCATTAGAAAGAAGATTGAGCCGGAGGCCGTACTGCAGACCAGAGTCTCCGCTATGGATAACACCC
  AGGAGAACAGTCTCAAGTCCGGGAAGGGGGCAGCTGCCATGATCCCAGGCCCACAGACGGTGGCCACGGA
  AATCCGTTCTCTTTCTCCGATCATCGGGAAAGATGTCCTCACCAGCACCTACGGCGCCACTGCGGAAACC
  CTCTCAACCTCCACCACCACCCATGTCACCAAAACTGTGAAAGGAGGGTTTTCTGAGACAAGGATCGAGA
  AGCGAATCATCATTACTGGGGATGAAGATGTCGATCAAGACCAGGCCCTGGCTTTGGCCATCAAGGAGGC
  CAAACTGCAGCATCCTGATATGCTGGTAACCAAAGCTGTCGTATACAGAGAAACAGACCCATCCCCAGAG
  GAGAGGGACAAGAAGCCACAGGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202596 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEKDYSEADGLSERTTPSKAQKSPQKIAKKYKSAICRVTLLDASEYECEVEKHGRGQVLFDLVCEHLNL
  LEKDYFGLTFCDADSQKNWLDPSKEIKKQIRSSPWNFAFTVKFYPPDPAQLTEDITRYYLCLQLRADIIT
  GRLPCSFVTHALLGSYAVQAELGDYDAEEHVGNYVSELRFAPNQTRELEERIMELHKTYRGMTPGEAEIH
  FLENAKKLSMYGVDLHHAKDSEGIDIMLGVCANGLLIYRDRLRINRFAWPKILKISYKRSNFYIKIRPGE
  YEQFESTIGFKLPNHRSAKRLWKVCIEHHTFFRLVSPEPPPKGFLVMGSKFRYSGRTQAQTRQASALIDR
  PAPFFERSSSKRYTMSRSLDGAEFSRPASVSENHDAGPDGDKRDEDGESGGQRSEAEEGEVRTPTKIKEL
  KFLDKPEDVLLKHQASINELKRTLKEPNSKLIHRDRDWERERRLPSSPASPSPKGTPEKANERAGLREGS
  EEKVKPPRPRAPESDTGDEDQDQERDTVFLKDNHLAIERKCSSITVSSTSSLEAEVDFTVIGDYHGSAFE
  DFSRSLPELDRDKSDSDTEGLLFSRDLNKGAPSQDDESGGIEDSPDRGACSTPDMPQFEPVKTETMTVSS
  LAIRKKIEPEAVLQTRVSAMDNTQENSLKSGKGAAAMIPGPQTVATEIRSLSPIIGKDVLTSTYGATAET
  LSTSTTTHVTKTVKGGFSETRIEKRIIITGDEDVDQDQALALAIKEAKLQHPDMLVTKAVVYRETDPSPE
  ERDKKPQES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177996
• ORF Size: 2337 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177996.1, NM_177996.2, NP_818932.1
• RefSeq Size: 5967 bp
• RefSeq ORF: 2340 bp
• Locus ID: 2036
• Cytogenetics: 20q11.23
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• MW: 87.6 kDa
• Gene Summary: 'Erythrocyte membrane protein band 4.1 (EPB41) is a multifunctional protein that mediates interactions between the erythrocyte cytoskeleton and the overlying plasma membrane. The encoded protein binds and stabilizes D2 and D3 dopamine receptors at the neuronal plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]'