ABHD3 (NM_138340) Human Tagged ORF Clone

CAT#: RC202633

ABHD3 (Myc-DDK-tagged)-Human abhydrolase domain containing 3 (ABHD3)

Product Images

Western blot validation of overexpression lysate (Cat# LY408617) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202633 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ABHD3 protein (Cat# TP302633). The protein was produced from HEK293T cells transfected with ABHD3 cDNA clone (Cat# RC202633) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABHD3
• Synonyms: LABH3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202633 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTGCCTGGCCATGGACCTGCGGATGTTGTCCCGGGAGCTCTCCCTCTACCTGGAACACCAAGTCC
  GGGTGGGGTTCTTCGGCTCGGGGGTGGGCTTATCCCTTATCCTGGGCTTCAGCGTCGCTTATGCCTTCTA
  CTACCTGAGCAGCATTGCCAAGAAACCCCAGTTAGTGACCGGGGGTGAGAGTTTCAGCCGCTTCCTTCAA
  GACCACTGTCCCGTGGTTACAGAAACGTACTACCCGACGGTCTGGTGCTGGGAGGGTCGAGGACAGACCC
  TGCTTAGACCTTTCATCACTTCGAAGCCCCCGGTGCAGTACAGGAATGAACTTATTAAAACTGCAGATGG
  AGGACAGATTTCACTGGACTGGTTTGATAATGATAACAGTACGTGTTATATGGATGCCAGCACCAGACCT
  ACTATCTTATTGTTGCCTGGCCTCACGGGAACAAGCAAGGAGTCATATATCCTTCATATGATCCATCTTA
  GTGAAGAATTAGGATACAGATGTGTGGTTTTTAACAACAGAGGAGTGGCGGGGGAGAATCTCTTGACGCC
  AAGGACTTATTGTTGTGCTAACACTGAAGACTTGGAGACAGTTATTCACCATGTACACAGCCTGTACCCT
  TCTGCTCCTTTCCTGGCAGCAGGGGTTTCAATGGGAGGAATGCTGCTTCTAAATTACTTGGGCAAAATTG
  GGTCCAAAACGCCTTTGATGGCAGCTGCAACTTTTTCCGTTGGTTGGAACACCTTCGCTTGCTCAGAGTC
  ATTGGAAAAACCACTGAACTGGCTACTTTTTAATTACTATTTGACAACCTGCCTTCAGTCTTCAGTTAAT
  AAGCACCGACATATGTTTGTAAAACAAGTTGATATGGATCATGTCATGAAGGCTAAATCCATCAGAGAGT
  TTGATAAGCGATTCACTTCAGTCATGTTTGGATACCAAACAATTGATGATTATTATACTGATGCCAGTCC
  GAGTCCTAGACTGAAGTCAGTAGGAATTCCAGTATTGTGTCTAAATTCTGTGGATGATGTTTTCTCACCC
  AGTCATGCTATTCCAATAGAAACTGCTAAGCAAAATCCTAATGTTGCTTTGGTCCTTACTTCTTATGGAG
  GCCATATTGGTTTTCTGGAGGGAATCTGGCCAAGACAGTCCACTTACATGGATCGTGTCTTCAAGCAATT
  TGTGCAAGCCATGGTTGAGCATGGACATGAACTCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202633 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQCLAMDLRMLSRELSLYLEHQVRVGFFGSGVGLSLILGFSVAYAFYYLSSIAKKPQLVTGGESFSRFLQ
  DHCPVVTETYYPTVWCWEGRGQTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNDNSTCYMDASTRP
  TILLLPGLTGTSKESYILHMIHLSEELGYRCVVFNNRGVAGENLLTPRTYCCANTEDLETVIHHVHSLYP
  SAPFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLEKPLNWLLFNYYLTTCLQSSVN
  KHRHMFVKQVDMDHVMKAKSIREFDKRFTSVMFGYQTIDDYYTDASPSPRLKSVGIPVLCLNSVDDVFSP
  SHAIPIETAKQNPNVALVLTSYGGHIGFLEGIWPRQSTYMDRVFKQFVQAMVEHGHELS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138340
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138340.1, NM_138340.2, NM_138340.3, NM_138340.4, NP_612213.2
• RefSeq Size: 2085 bp
• RefSeq ORF: 1230 bp
• Locus ID: 171586
• Protein Families: Transmembrane
• MW: 46 kDa
• Gene Summary: This gene encodes a protein containing an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. The function of this protein has not been determined. [provided by RefSeq, Jul 2008]