RING1 (NM_002931) Human Tagged ORF Clone

CAT#: RC202650

RING1 (Myc-DDK-tagged)-Human ring finger protein 1 (RING1)

Product Images

Western blot validation of overexpression lysate (Cat# LY401023) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202650 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RING1 protein (Cat# TP302650). The protein was produced from HEK293T cells transfected with RING1 cDNA clone (Cat# RC202650) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RING1
• Synonyms: RING1A; RNF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202650 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACGCCGGCGAATGCCCAGAATGCCAGCAAAACGTGGGAACTGAGTCTGTATGAGCTGCACCGGA
  CCCCGCAGGAAGCCATAATGGATGGCACAGAGATTGCTGTTTCCCCTCGGTCACTGCATTCAGAACTCAT
  GTGCCCTATCTGCCTGGACATGCTGAAGAATACGATGACCACCAAGGAGTGCCTCCACAGATTCTGCTCT
  GACTGCATTGTCACAGCCCTACGGAGCGGGAACAAGGAGTGTCCTACCTGCCGAAAGAAGCTGGTGTCCA
  AGCGATCCCTACGGCCAGACCCCAACTTTGATGCCCTGATCTCTAAGATCTATCCTAGCCGGGAGGAATA
  CGAGGCCCATCAAGACCGAGTGCTTATCCGCCTGAGCCGCCTGCACAACCAGCAGGCATTGAGCTCCAGC
  ATTGAGGAGGGGCTACGCATGCAGGCCATGCACAGGGCCCAGCGTGTGAGGCGGCCGATACCAGGGTCAG
  ATCAGACCACAACGATGAGTGGGGGGGAAGGAGAGCCCGGGGAGGGAGAAGGGGATGGAGAAGATGTGAG
  CTCAGACTCCGCCCCTGACTCTGCCCCAGGCCCTGCTCCCAAGCGACCCCGTGGAGGGGGCGCAGGGGGG
  AGCAGTGTAGGGACAGGGGGAGGCGGCACTGGTGGGGTGGGTGGGGGTGCCGGTTCGGAAGACTCTGGTG
  ACCGGGGAGGGACTCTGGGAGGGGGAACGCTGGGCCCCCCAAGCCCTCCTGGGGCCCCCAGCCCCCCAGA
  GCCAGGTGGAGAAATTGAGCTCGTGTTCCGGCCCCACCCCCTGCTCGTGGAGAAGGGAGAATACTGCCAG
  ACGAGGTATGTGAAGACAACTGGGAATGCCACAGTGGACCACCTCTCCAAGTACTTGGCCCTGCGCATTG
  CCCTCGAGCGGAGGCAACAGCAGGAAGCAGGGGAGCCAGGAGGGCCTGGAGGGGGCGCCTCTGACACCGG
  AGGACCTGATGGGTGTGGCGGGGAGGGTGGGGGTGCCGGAGGAGGTGACGGTCCTGAGGAGCCTGCTTTG
  CCCAGCCTGGAGGGCGTCAGTGAAAAGCAGTACACCATCTACATCGCACCTGGAGGCGGGGCGTTCACGA
  CGTTGAATGGCTCGCTGACCCTGGAGCTGGTGAATGAGAAATTCTGGAAGGTGTCCCGGCCACTGGAGCT
  GTGCTATGCTCCCACCAAGGATCCAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202650 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTTPANAQNASKTWELSLYELHRTPQEAIMDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCS
  DCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSS
  IEEGLRMQAMHRAQRVRRPIPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGG
  SSVGTGGGGTGGVGGGAGSEDSGDRGGTLGGGTLGPPSPPGAPSPPEPGGEIELVFRPHPLLVEKGEYCQ
  TRYVKTTGNATVDHLSKYLALRIALERRQQQEAGEPGGPGGGASDTGGPDGCGGEGGGAGGGDGPEEPAL
  PSLEGVSEKQYTIYIAPGGGAFTTLNGSLTLELVNEKFWKVSRPLELCYAPTKDPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002931
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002931.3, NP_002922.2
• RefSeq Size: 1744 bp
• RefSeq ORF: 1221 bp
• Locus ID: 6015
• Domains: RING
• Protein Families: Druggable Genome, Transcription Factors
• MW: 42.4 kDa
• Gene Summary: This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq, Jul 2008]