WIPI2 (NM_015610) Human Tagged ORF Clone

CAT#: RC202654

WIPI2 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414456) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202654 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WIPI2
• Synonyms: ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202654 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTGGCGAGCCAGAGCGGGGAGGCCGGCGCCGGCCAGCTGCTCTTCGCCAACTTCAACCAGGACA
  ACACAGAAGTGAAAGGGGCATCAAGAGCAGCTGGTCTTGGCCGTCGCGCTGTTGTCTGGTCCCTAGCTGT
  TGGTAGTAAGTCCGGTTATAAATTTTTCTCCCTTTCTTCTGTGGATAAGCTGGAACAGATCTATGAATGC
  ACCGATACGGAAGATGTGTGCATTGTAGAGAGATTGTTCTCCAGCAGCCTAGTGGCCATCGTGAGCCTTA
  AAGCACCAAGGAAGCTAAAGGTTTGCCACTTTAAGAAGGGAACTGAGATCTGCAACTACAGCTACTCCAA
  CACGATTCTGGCTGTGAAGCTCAACAGGCAGAGGCTGATAGTATGCCTGGAGGAGTCCCTGTACATCCAC
  AACATTCGGGACATGAAGGTGCTGCATACGATCAGGGAGACGCCTCCAAACCCTGCAGGCCTGTGTGCGC
  TGTCAATCAACAACGACAACTGCTACTTGGCGTACCCAGGGAGCGCGACCATCGGAGAGGTGCAGGTCTT
  CGATACCATTAATTTGAGAGCTGCAAACATGATTCCGGCTCACGACAGTCCTTTAGCGGCACTGGCCTTT
  GACGCAAGTGGAACTAAACTTGCCACGGCTTCGGAGAAGGGGACCGTGATTAGGGTATTTTCCATTCCAG
  AAGGACAAAAACTCTTTGAGTTTCGGAGAGGAGTAAAGAGGTGCGTGAGCATCTGCTCCCTGGCCTTCAG
  CATGGACGGCATGTTCCTCTCCGCCTCCAGCAACACTGAGACCGTGCACATCTTCAAACTCGAGACTGTG
  AAAGAAAAACCCCCAGAGGAGCCCACCACCTGGACCGGGTACTTCGGGAAAGTGCTCATGGCCTCCACCA
  GCTACCTGCCTTCCCAAGTGACAGAAATGTTCAACCAGGGCAGAGCCTTCGCCACGGTCCGCCTGCCATT
  CTGCGGCCACAAAAACATCTGCTCGCTAGCCACAATTCAGAAGATCCCGCGGTTGTTGGTGGGTGCCGCC
  GACGGGTACCTGTACATGTACAACCTGGACCCCCAGGAGGGCGGCGAGTGTGCCCTGATGAAGCAGCACC
  GGCTGGACGGCAGTCTGGAAACGACCAATGAGATCTTGGACTCTGCCTCTCACGACTGCCCCTTAGTCAC
  TCAGACATACGGCGCAGCTGCAGGAAAAGGTACTTACGTGCCTTCATCCCCAACGAGACTTGCCTACACA
  GACGACCTGGGTGCTGTGGGTGGCGCCTGCCTGGAGGACGAGGCCAGCGCCCTGCGCCTGGATGAGGACA
  GCGAGCACCCGCCCATGATTCTTCGGACTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202654 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNLASQSGEAGAGQLLFANFNQDNTEVKGASRAAGLGRRAVVWSLAVGSKSGYKFFSLSSVDKLEQIYEC
  TDTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIH
  NIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIPAHDSPLAALAF
  DASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLETV
  KEKPPEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATIQKIPRLLVGAA
  DGYLYMYNLDPQEGGECALMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKGTYVPSSPTRLAYT
  DDLGAVGGACLEDEASALRLDEDSEHPPMILRTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015610
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015610.1, NM_015610.2, NM_015610.3, NP_056425.1
• RefSeq Size: 4489 bp
• RefSeq ORF: 1365 bp
• Locus ID: 26100
• Domains: WD40
• MW: 49.4 kDa
• Gene Summary: WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]). [supplied by OMIM, Mar 2008]