WIPI2 (NM_015610) Human Tagged ORF Clone
CAT#: RC202654
WIPI2 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 1
"NM_015610" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | WIPI2 |
Synonyms | ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202654 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTGGCGAGCCAGAGCGGGGAGGCCGGCGCCGGCCAGCTGCTCTTCGCCAACTTCAACCAGGACA ACACAGAAGTGAAAGGGGCATCAAGAGCAGCTGGTCTTGGCCGTCGCGCTGTTGTCTGGTCCCTAGCTGT TGGTAGTAAGTCCGGTTATAAATTTTTCTCCCTTTCTTCTGTGGATAAGCTGGAACAGATCTATGAATGC ACCGATACGGAAGATGTGTGCATTGTAGAGAGATTGTTCTCCAGCAGCCTAGTGGCCATCGTGAGCCTTA AAGCACCAAGGAAGCTAAAGGTTTGCCACTTTAAGAAGGGAACTGAGATCTGCAACTACAGCTACTCCAA CACGATTCTGGCTGTGAAGCTCAACAGGCAGAGGCTGATAGTATGCCTGGAGGAGTCCCTGTACATCCAC AACATTCGGGACATGAAGGTGCTGCATACGATCAGGGAGACGCCTCCAAACCCTGCAGGCCTGTGTGCGC TGTCAATCAACAACGACAACTGCTACTTGGCGTACCCAGGGAGCGCGACCATCGGAGAGGTGCAGGTCTT CGATACCATTAATTTGAGAGCTGCAAACATGATTCCGGCTCACGACAGTCCTTTAGCGGCACTGGCCTTT GACGCAAGTGGAACTAAACTTGCCACGGCTTCGGAGAAGGGGACCGTGATTAGGGTATTTTCCATTCCAG AAGGACAAAAACTCTTTGAGTTTCGGAGAGGAGTAAAGAGGTGCGTGAGCATCTGCTCCCTGGCCTTCAG CATGGACGGCATGTTCCTCTCCGCCTCCAGCAACACTGAGACCGTGCACATCTTCAAACTCGAGACTGTG AAAGAAAAACCCCCAGAGGAGCCCACCACCTGGACCGGGTACTTCGGGAAAGTGCTCATGGCCTCCACCA GCTACCTGCCTTCCCAAGTGACAGAAATGTTCAACCAGGGCAGAGCCTTCGCCACGGTCCGCCTGCCATT CTGCGGCCACAAAAACATCTGCTCGCTAGCCACAATTCAGAAGATCCCGCGGTTGTTGGTGGGTGCCGCC GACGGGTACCTGTACATGTACAACCTGGACCCCCAGGAGGGCGGCGAGTGTGCCCTGATGAAGCAGCACC GGCTGGACGGCAGTCTGGAAACGACCAATGAGATCTTGGACTCTGCCTCTCACGACTGCCCCTTAGTCAC TCAGACATACGGCGCAGCTGCAGGAAAAGGTACTTACGTGCCTTCATCCCCAACGAGACTTGCCTACACA GACGACCTGGGTGCTGTGGGTGGCGCCTGCCTGGAGGACGAGGCCAGCGCCCTGCGCCTGGATGAGGACA GCGAGCACCCGCCCATGATTCTTCGGACTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202654 protein sequence
Red=Cloning site Green=Tags(s) MNLASQSGEAGAGQLLFANFNQDNTEVKGASRAAGLGRRAVVWSLAVGSKSGYKFFSLSSVDKLEQIYEC TDTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIH NIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIPAHDSPLAALAF DASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLETV KEKPPEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATIQKIPRLLVGAA DGYLYMYNLDPQEGGECALMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKGTYVPSSPTRLAYT DDLGAVGGACLEDEASALRLDEDSEHPPMILRTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015610 |
ORF Size | 1362 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015610.1, NM_015610.2, NM_015610.3, NP_056425.1 |
RefSeq Size | 4489 bp |
RefSeq ORF | 1365 bp |
Locus ID | 26100 |
Domains | WD40 |
MW | 49.4 kDa |
Gene Summary | WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]). [supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC114682 | WIPI2 (untagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 1 |
USD 760.00 |
|
SC320341 | WIPI2 (untagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 1 |
USD 760.00 |
|
RG202654 | WIPI2 (GFP-tagged) - Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 1 |
USD 460.00 |
|
RC202654L3 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC202654L4 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 1, mGFP tagged |
USD 620.00 |
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