Nucleoside Diphosphate Kinase 7 (NME7) (NM_197972) Human Tagged ORF Clone

CAT#: RC202669

NME7 (Myc-DDK-tagged)-Human non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase) (NME7), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405095) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202669 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NME7 protein (Cat# TP302669). The protein was produced from HEK293T cells transfected with NME7 cDNA clone (Cat# RC202669) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NME7
• Synonyms: CFAP67; MN23H7; NDK 7; NDK7; nm23-H7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202669 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCATAGTGAAAGATTCGTTTTCATTGCAGAGTGGTATGATCCAAATGCTTCACTTCTTCGACGTT
  ATGAGCTTTTATTTTACCCAGGGGATGGATCTGTTGAAATGCATGATGTAAAGAATCATCGCACCTTTTT
  AAAGCGGACCAAATATGATAACCTGCACTTGGAAGATTTATTTATAGGCAACAAAGTGAATGTCTTCTCT
  CGACAACTGGTATTAATTGACTATGGGGATCAATATACAGCTCGCCAGCTGGGCAGTAGGAAAGAAAAAA
  CGCTAGCCCTAATTAAACCAGATGCAATATCAAAGGCTGGAGAAATAATTGAAATAATAAACAAAGCTGG
  ATTTACTATAACCAAACTCAAAATGATGATGCTTTCAAGGAAAGAAGCATTGGATTTTCATGTAGATCAC
  CAGTCAAGACCCTTTTTCAATGAGCTGATCCAGTTTATTACAACTGGTCCTATTATTGCCATGGAGATTT
  TAAGAGATGATGCTATATGTGAATGGAAAAGACTGCTGGGACCTGCAAACTCTGGAGTGGCACGCACAGA
  TGCTTCTGAAAGCATTAGAGCCCTCTTTGGAACAGATGGCATAAGAAATGCAGCGCATGGCCCTGATTCT
  TTTGCTTCTGCGGCCAGAGAAATGGAGTTGTTTTTTCCTTCAAGTGGAGGTTGTGGGCCGGCAAACACTG
  CTAAATTTACTAATTGTACCTGTTGCATTGTTAAACCCCATGCTGTCAGTGAAGGACTGTTGGGAAAGAT
  CCTGATGGCTATCCGAGATGCAGGTTTTGAAATCTCAGCTATGCAGATGTTCAATATGGATCGGGTTAAT
  GTTGAGGAATTCTATGAAGTTTATAAAGGAGTAGTGACCGAATATCATGACATGGTGACAGAAATGTATT
  CTGGCCCTTGTGTAGCAATGGAGATTCAACAGAATAATGCTACAAAGACATTTCGAGAATTTTGTGGACC
  TGCTGATCCTGAAATTGCCCGGCATTTACGCCCTGGAACTCTCAGAGCAATCTTTGGTAAAACTAAGATC
  CAGAATGCTGTTCACTGTACTGATCTGCCAGAGGATGGCCTATTAGAGGTTCAATACTTCTTCAAGATCT
  TGGATAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202669 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNHSERFVFIAEWYDPNASLLRRYELLFYPGDGSVEMHDVKNHRTFLKRTKYDNLHLEDLFIGNKVNVFS
  RQLVLIDYGDQYTARQLGSRKEKTLALIKPDAISKAGEIIEIINKAGFTITKLKMMMLSRKEALDFHVDH
  QSRPFFNELIQFITTGPIIAMEILRDDAICEWKRLLGPANSGVARTDASESIRALFGTDGIRNAAHGPDS
  FASAAREMELFFPSSGGCGPANTAKFTNCTCCIVKPHAVSEGLLGKILMAIRDAGFEISAMQMFNMDRVN
  VEEFYEVYKGVVTEYHDMVTEMYSGPCVAMEIQQNNATKTFREFCGPADPEIARHLRPGTLRAIFGKTKI
  QNAVHCTDLPEDGLLEVQYFFKILDN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_197972
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1625 bp
• RefSeq ORF: 1023 bp
• Locus ID: 29922
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• MW: 42.5 kDa
• Gene Summary: This gene encodes a member of the non-metastatic expressed family of nucleoside diphosphate kinases. Members of this family are enzymes that catalyzes phosphate transfer from nucleoside triphosphates to nucleoside diphosphates. This protein contains two kinase domains, one of which is involved in autophosphorylation and the other may be inactive. This protein localizes to the centrosome and functions as a component of the gamma-tubulin ring complex which plays a role in microtubule organization. Mutations in this gene may be associated with venous thromboembolism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]