PRIM1 (NM_000946) Human Tagged ORF Clone
CAT#: RC202710L3
- LentiORF®
Lenti ORF clone of Human primase, DNA, polypeptide 1 (49kDa) (PRIM1), Myc-DDK-tagged
"NM_000946" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PRIM1 |
Synonyms | p49 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC202710).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000946 |
ORF Size | 1260 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000946.2, NP_000937.1 |
RefSeq Size | 1471 bp |
RefSeq ORF | 1263 bp |
Locus ID | 5557 |
Cytogenetics | 12q13.3 |
Protein Families | Druggable Genome |
Protein Pathways | DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism |
MW | 49.9 kDa |
Gene Summary | 'The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC126303 | PRIM1 (untagged)-Human primase, DNA, polypeptide 1 (49kDa) (PRIM1) |
USD 310.00 |
|
RC202710 | PRIM1 (Myc-DDK-tagged)-Human primase, DNA, polypeptide 1 (49kDa) (PRIM1) |
USD 420.00 |
|
RG202710 | PRIM1 (GFP-tagged) - Human primase, DNA, polypeptide 1 (49kDa) (PRIM1) |
USD 460.00 |
|
RC202710L4 | Lenti ORF clone of Human primase, DNA, polypeptide 1 (49kDa) (PRIM1), mGFP tagged |
USD 620.00 |
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