PRIM1 (NM_000946) Human Untagged Clone

CAT#: SC126303

PRIM1 (untagged)-Human primase, DNA, polypeptide 1 (49kDa) (PRIM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRIM1
• Synonyms: p49
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000946 edited
  CCTGTGGTAATCCTTACCGTGGCGAGTTCCGCGCTCAATGGAGACGTTTGACCCCACCGA
  GCTGCCCGAGCTGCTTAAACTTTATTACCGGAGGCTCTTTCCCTACTCTCAGTACTATCG
  CTGGCTCAACTACGGTGGAGTGATAAAGAATTACTTTCAACACCGTGAATTTTCATTCAC
  ATTGAAAGATGATATTTACATTCGCTACCAATCCTTCAACAACCAGAGTGATCTGGAAAA
  GGAGATGCAGAAAATGAATCCATACAAGATTGATATAGGCGCAGTATATTCTCACAGACC
  CAATCAACACAATACAGTGAAGCTGGGAGCTTTCCAGGCTCAGGAAAAAGAACTGGTATT
  TGACATTGACATGACAGACTATGACGATGTGAGGAGATGTTGTAGTTCTGCAGACATATG
  TCCTAAGTGCTGGACCCTCATGACAATGGCCATACGCATCATTGACAGAGCATTGAAGGA
  GGACTTTGGATTTAAGCATCGTCTCTGGGTATATTCTGGAAGGAGAGGTGTTCATTGTTG
  GGTCTGTGATGAATCAGTTAGAAAACTGTCTTCTGCAGTACGTTCTGGGATAGTTGAGTA
  TTTGAGCCTTGTAAAGGGTGGTCAAGACGTTAAAAAGAAAGTTCACCTAAGTGAAAAAAT
  TCACCCTTTTATCAGAAAATCTATAAACATAATAAAAAAATACTTTGAAGAATATGCCTT
  GGTTAATCAAGATATTCTCGAAAATAAAGAAAGCTGGGATAAGATTTTAGCCCTTGTTCC
  TGAAACAATTCATGATGAACTTCAACAAAGCTTCCAAAAGTCTCACAATTCACTTCAGCG
  TTGGGAGCACTTGAAGAAAGTAGCCAGCAGATATCAGAATAACATCAAAAATGACAAATA
  TGGACCCTGGCTGGAGTGGGAGATTATGCTCCAGTACTGTTTTCCACGGCTGGATATCAA
  TGTCAGCAAAGGAATCAATCATCTACTGAAGAGCCCTTTTAGTGTTCATCCTAAAACAGG
  TCGCATATCTGTGCCTATTGATTTGCAGAAAGTGGACCAGTTTGATCCATTTACTGTTCC
  GACCATAAGCTTCATCTGCCGTGAATTGGATGCCATTTCCACTAATGAAGAGGAAAAAGA
  GGAGAATGAAGCTGAATCTGATGTCAAACATAGAACCAGAGATTATAAGAAGACCAGTCT
  AGCACCTTATGTGAAAGTTTTTGAACATTTTCTTGAAAATCTGGATAAATCCCGAAAAGG
  AGAACTTCTTAAGAAGAGTGATTTACAAAAAGATTTCTGAAGACAGAGCTCCTCAAACCA
  TTGTGGATATCTTCTGCCTTCAACCACAGATCAAATACTTCAAGAGCCATTTAATAAATA
  TGGCAGAACTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000946 unedited
  TCAGGATTTGTATACGACTGCACTATAGGCGGCGCGCGAATTCGCCATTACGGCCGGGGC
  CTGTGGTAATCCTTACCGTGGCGAGTTCCGCGCTCAATGGAGACGTTTGACCCCACCGAG
  CTGCCCGAGCTGCTTAAACTTTATTACCGGAGGCTCTTTCCCTACTCTCAGTACTATCGC
  TGGCTCAACTACGGTGGAGTGATAAAGAATTACTTTCAACACCGTGAATTTTCATTCACA
  TGGGGAAGATGATATTTACATTCGCTACCAATCCTTCAACAACCAGAGTGATCTGGAAAA
  GGAGATGCAGAAAATGAATCCATACAAGATTGATATAGGCGCAGTATATTCTCACAGACC
  CAATCAACACAATACAGTGAAGCTGGGAGCTTTCCAGGCTCAGGAAAAAGAACTGGTATT
  TGACATTGACATGACAGACTATGACGATGTGAGGAGATGTTGTANTTCTGCAAACATATG
  TCCTAAGTGCTGGGACCCTCATGACAATGGCCATACGCATCATTGACAGAGCATTGAAGG
  AGGACTTTGGATTTAAGCATCGGCTCTGGGTATATTCTGGAAGAGAGGTGTTCATTGGTN
  GGTCTGTGATGAATCAGTTAGAAAACTGTGCTCTGCAGTACTTTCTGGGATAGTTGAGTA
  TTTGAGCCTTGGAAAGGTGGGCAAGACGTAAAAAGAAAGTTACCTAGTGAAAAAATCCCC
  CCTTTATCGGANATCTTTAACCTATAAAAAATCCTTTGAGAATATGCTTTGGTAATAAGA
  ATTCTCCAAATAAGAAACGTGGGTAGGATTTACCCCTGGGCCTGAAACATTCATGAGGAA
  TTCAACAAACTCCCAAAGCTAAAATTCCTTCACGGTTGGGGCCTTAAAAAATGCCCCCGA
  ATTCAAAACCTCCAAATAACAATGGCGCCGGCGGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_000946
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000946.2, NP_000937.1
• RefSeq Size: 1471 bp
• RefSeq ORF: 1263 bp
• Locus ID: 5557
• Cytogenetics: 12q13.3
• Protein Families: Druggable Genome
• Protein Pathways: DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• Gene Summary: 'The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008]'