Hey L (HEYL) (NM_014571) Human Tagged ORF Clone
CAT#: RC202851
HEYL (Myc-DDK-tagged)-Human hairy/enhancer-of-split related with YRPW motif-like (HEYL)
"NM_014571" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HEYL |
Synonyms | bHLHb33; HESR3; HEY3; HRT3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202851 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGACCCAAGGAGCCGAGCGGCTCCGACGGGGAGTCCGACGGACCCATCGACGTGGGCCAAGAGG GCCAGCTGAGCCAGATGGCCAGGCCGCTGTCCACCCCCAGCTCTTCGCAGATGCAAGCCAGGAAGAAACG CAGAGGGATCATAGAGAAACGGCGTCGAGACCGCATCAACAGTAGCCTTTCTGAATTGCGACGCTTGGTC CCCACTGCCTTTGAGAAACAGGGCTCTTCCAAGCTGGAGAAAGCCGAGGTCTTGCAGATGACGGTGGATC ACTTGAAAATGCTCCATGCCACTGGTGGGACAGGATTCTTTGATGCCCGAGCCCTGGCAGTTGACTTCCG GAGCATTGGTTTTCGGGAGTGCCTCACTGAGGTCATCAGGTACCTGGGGGTCCTTGAAGGGCCCAGCAGC CGTGCAGACCCCGTCCGGATTCGCCTTCTCTCCCACCTCAACAGCTACGCAGCCGAGATGGAGCCTTCGC CCACGCCCACTGGCCCTTTGGCCTTCCCTGCCTGGCCCTGGTCTTTCTTCCATAGCTGTCCAGGGCTGCC AGCCCTGAGCAACCAGCTCGCCATCCTGGGAAGAGTGCCCAGCCCTGTCCTCCCCGGTGTCTCCTCTCCT GCTTACCCCATCCCAGCCCTCCGAACCGCTCCCCTTCGCAGAGCCACAGGCATCATCCTGCCAGCCCGGA GGAATGTGCTGCCCAGTCGAGGGGCATCTTCCACCCGGAGGGCCCGCCCCCTAGAGAGGCCAGCGACCCC TGTGCCTGTCGCCCCCAGCAGCAGGGCTGCCAGGAGCAGCCACATCGCTCCCCTCCTGCAGTCTTCCTCC CCAACACCCCCTGGTCCTACAGGGTCGGCTGCTTACGTGGCTGTTCCCACCCCCAACTCATCCTCCCCAG GGCCAGCTGGGAGGCCAGCGGGAGCCATGCTCTACCACTCCTGGGTCTCTGAAATCACTGAAATCGGGGC TTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202851 protein sequence
Red=Cloning site Green=Tags(s) MKRPKEPSGSDGESDGPIDVGQEGQLSQMARPLSTPSSSQMQARKKRRGIIEKRRRDRINSSLSELRRLV PTAFEKQGSSKLEKAEVLQMTVDHLKMLHATGGTGFFDARALAVDFRSIGFRECLTEVIRYLGVLEGPSS RADPVRIRLLSHLNSYAAEMEPSPTPTGPLAFPAWPWSFFHSCPGLPALSNQLAILGRVPSPVLPGVSSP AYPIPALRTAPLRRATGIILPARRNVLPSRGASSTRRARPLERPATPVPVAPSSRAARSSHIAPLLQSSS PTPPGPTGSAAYVAVPTPNSSSPGPAGRPAGAMLYHSWVSEITEIGAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014571 |
ORF Size | 984 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014571.1, NM_014571.2, NM_014571.3, NP_055386.1 |
RefSeq Size | 4131 bp |
RefSeq ORF | 987 bp |
Locus ID | 26508 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 35.1 kDa |
Gene Summary | This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC122816 | HEYL (untagged)-Human hairy/enhancer-of-split related with YRPW motif-like (HEYL) |
USD 310.00 |
|
RG202851 | HEYL (GFP-tagged) - Human hairy/enhancer-of-split related with YRPW motif-like (HEYL) |
USD 460.00 |
|
RC202851L1 | Lenti ORF clone of Human hairy/enhancer-of-split related with YRPW motif-like (HEYL), Myc-DDK-tagged |
USD 768.00 |
|
RC202851L2 | Lenti ORF clone of Human hairy/enhancer-of-split related with YRPW motif-like (HEYL), mGFP tagged |
USD 768.00 |
|
RC202851L3 | Lenti ORF clone of Human hairy/enhancer-of-split related with YRPW motif-like (HEYL), Myc-DDK-tagged |
USD 620.00 |
|
RC202851L4 | Lenti ORF clone of Human hairy/enhancer-of-split related with YRPW motif-like (HEYL), mGFP tagged |
USD 620.00 |
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