Hey L (HEYL) (NM_014571) Human Tagged ORF Clone

CAT#: RG202851

HEYL (GFP-tagged) - Human hairy/enhancer-of-split related with YRPW motif-like (HEYL)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HEYL
• Synonyms: bHLHb33; HESR3; HEY3; HRT3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202851 representing NM_014571
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGACCCAAGGAGCCGAGCGGCTCCGACGGGGAGTCCGACGGACCCATCGACGTGGGCCAAGAGG
  GCCAGCTGAGCCAGATGGCCAGGCCGCTGTCCACCCCCAGCTCTTCGCAGATGCAAGCCAGGAAGAAACG
  CAGAGGGATCATAGAGAAACGGCGTCGAGACCGCATCAACAGTAGCCTTTCTGAATTGCGACGCTTGGTC
  CCCACTGCCTTTGAGAAACAGGGCTCTTCCAAGCTGGAGAAAGCCGAGGTCTTGCAGATGACGGTGGATC
  ACTTGAAAATGCTCCATGCCACTGGTGGGACAGGATTCTTTGATGCCCGAGCCCTGGCAGTTGACTTCCG
  GAGCATTGGTTTTCGGGAGTGCCTCACTGAGGTCATCAGGTACCTGGGGGTCCTTGAAGGGCCCAGCAGC
  CGTGCAGACCCCGTCCGGATTCGCCTTCTCTCCCACCTCAACAGCTACGCAGCCGAGATGGAGCCTTCGC
  CCACGCCCACTGGCCCTTTGGCCTTCCCTGCCTGGCCCTGGTCTTTCTTCCATAGCTGTCCAGGGCTGCC
  AGCCCTGAGCAACCAGCTCGCCATCCTGGGAAGAGTGCCCAGCCCTGTCCTCCCCGGTGTCTCCTCTCCT
  GCTTACCCCATCCCAGCCCTCCGAACCGCTCCCCTTCGCAGAGCCACAGGCATCATCCTGCCAGCCCGGA
  GGAATGTGCTGCCCAGTCGAGGGGCATCTTCCACCCGGAGGGCCCGCCCCCTAGAGAGGCCAGCGACCCC
  TGTGCCTGTCGCCCCCAGCAGCAGGGCTGCCAGGAGCAGCCACATCGCTCCCCTCCTGCAGTCTTCCTCC
  CCAACACCCCCTGGTCCTACAGGGTCGGCTGCTTACGTGGCTGTTCCCACCCCCAACTCATCCTCCCCAG
  GGCCAGCTGGGAGGCCAGCGGGAGCCATGCTCTACCACTCCTGGGTCTCTGAAATCACTGAAATCGGGGC
  TTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202851 representing NM_014571
  Red=Cloning site Green=Tags(s)
  
  MKRPKEPSGSDGESDGPIDVGQEGQLSQMARPLSTPSSSQMQARKKRRGIIEKRRRDRINSSLSELRRLV
  PTAFEKQGSSKLEKAEVLQMTVDHLKMLHATGGTGFFDARALAVDFRSIGFRECLTEVIRYLGVLEGPSS
  RADPVRIRLLSHLNSYAAEMEPSPTPTGPLAFPAWPWSFFHSCPGLPALSNQLAILGRVPSPVLPGVSSP
  AYPIPALRTAPLRRATGIILPARRNVLPSRGASSTRRARPLERPATPVPVAPSSRAARSSHIAPLLQSSS
  PTPPGPTGSAAYVAVPTPNSSSPGPAGRPAGAMLYHSWVSEITEIGAF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014571
• ORF Size: 984 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014571.2, NP_055386.1
• RefSeq Size: 3416
• RefSeq ORF: 987
• Locus ID: 26508
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]