GNG3 (NM_012202) Human Tagged ORF Clone

CAT#: RC202857L3

Lenti ORF clone of Human guanine nucleotide binding protein (G protein), gamma 3 (GNG3), Myc-DDK-tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GNG3
• Synonyms: guanine nucleotide-binding protein gamma-3 subunit; guanine nucleotide binding protein (G protein), gamma 3; NBP gamma-3
• Vector: pLenti-C-Myc-DDK-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC202857).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_012202
• ORF Size: 225 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012202.1, NP_036334.1
• RefSeq Size: 962 bp
• RefSeq ORF: 228 bp
• Locus ID: 2785
• Cytogenetics: 11q12.3
• Domains: G-gamma
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Taste transduction
• MW: 8.3 kDa
• Gene Summary: 'Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The gamma subunit determines the specificity of which signaling pathways will be affected by this particular complex. The protein encoded by this gene represents the gamma subunit of both inhibitory and stimulatory complexes. [provided by RefSeq, Jan 2012]'