MCC (NM_002387) Human Tagged ORF Clone
CAT#: RC202890
MCC (Myc-DDK-tagged)-Human mutated in colorectal cancers (MCC), transcript variant 2
"NM_002387" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MCC |
Synonyms | MCC1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202890 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCCGGAGTTGCCATGAAATATGGAAACGACTCCTCGGCCGAGCTGAGTGAGCTCCATTCAGCAG CCCTGGCATCACTAAAGGGAGATATAGTGGAACTTAATAAACGTCTCCAGCAAACAGAGAGGGAACGGGA CCTTCTGGAAAAGAAATTGGCCAAGGCACAGTGCGAGCAGTCCCACCTCATGAGAGAGCATGAGGATGTC CAGGAGCGAACGACGCTTCGCTATGAGGAACGCATCACAGAGCTCCACAGCGTCATTGCGGAGCTCAACA AGAAGATAGACCGTCTGCAAGGCACCACCATCAGGGAGGAAGATGAGTACTCAGAACTGCGATCAGAACT CAGCCAGAGCCAACACGAGGTCAACGAGGACTCTCGAAGCATGGACCAAGACCAGACCTCTGTCTCTATC CCCGAAAACCAGTCTACCATGGTTACTGCTGACATGGACAACTGCAGTGACCTGAACTCAGAACTGCAGA GGGTGCTGACAGGGCTGGAGAATGTTGTCTGCGGCAGGAAGAAGAGCAGCTGCAGCCTCTCCGTGGCCGA GGTGGACAGGCACATTGAGCAGCTCACCACAGCCAGCGAGCACTGTGACCTGGCTATTAAGACAGTCGAG GAGATTGAGGGGGTGCTTGGCCGGGACCTGTATCCCAACCTGGCTGAAGAGAGGTCTCGGTGGGAGAAGG AGCTGGCTGGGCTGAGGGAAGAGAATGAGAGCCTGACTGCCATGCTGTGCAGCAAAGAGGAAGAACTGAA CCGGACTAAGGCCACCATGAATGCCATCCGGGAAGAGCGGGACCGGCTCCGGAGGAGGGTCAGAGAGCTT CAAACTCGACTACAGAGCGTGCAGGCCACAGGTCCCTCCAGCCCTGGCCGCCTCACTTCCACCAACCGCC CGATTAACCCCAGCACTGGGGAGCTGAGCACAAGCAGCAGCAGCAATGACATTCCCATCGCCAAGATTGC TGAGAGGGTGAAGCTATCAAAGACAAGGTCCGAATCGTCATCATCTGATCGGCCAGTCCTGGGCTCAGAA ATCAGTAGCATAGGGGTATCCAGCAGTGTGGCTGAACACCTGGCCCACTCACTTCAGGACTGCTCCAATA TCCAAGAGATTTTCCAAACACTCTACTCACACGGATCTGCCATCTCAGAAAGCAAGATTAGAGAGTTTGA GGTGGAAACAGAACGGCTGAATAGCCGGATTGAGCACCTCAAATCCCAAAATGACCTCCTGACCATAACC TTGGAGGAATGTAAAAGCAATGCCGAGAGGATGAGCATGCTGGTGGGAAAATACGAATCCAATGCCACAG CGCTGAGGCTGGCCTTGCAGTACAGCGAGCAGTGCATCGAAGCCTACGAACTCCTCCTGGCGCTGGCAGA GAGTGAGCAGAGCCTCATCCTGGGGCAGTTCCGAGCGGCGGGCGTGGGGTCCTCCCCTGGAGACCAGTCG GGGGATGAAAACATCACTCAGATGCTCAAGCGAGCTCATGACTGCCGGAAGACAGCTGAGAATGCTGCCA AGGCCCTGCTCATGAAGCTGGACGGCAGCTGTGGGGGAGCCTTTGCCGTGGCCGGCTGCAGCGTGCAGCC CTGGGAGAGCCTTTCCTCCAACAGCCACACCAGCACAACCAGCTCCACAGCCAGTAGTTGCGACACCGAG TTCACTAAAGAAGACGAGCAGAGGCTGAAGGATTATATCCAGCAGCTCAAGAATGACAGGGCTGCGGTCA AGCTGACCATGCTGGAGCTGGAAAGCATCCACATCGATCCTCTCAGCTATGACGTCAAGCCTCGGGGAGA CAGCCAGAGGCTGGATCTGGAAAACGCAGTGCTTATGCAGGAGCTCATGGCCATGAAGGAGGAGATGGCC GAGTTGAAGGCCCAGCTCTACCTACTGGAGAAAGAGAAGAAGGCCCTGGAGCTGAAGCTGAGCACGCGGG AGGCCCAGGAGCAGGCCTACCTGGTGCACATTGAGCACCTGAAGTCCGAGGTGGAGGAGCAGAAGGAGCA GCGGATGCGATCCCTCAGCTCCACCAGCAGCGGCAGCAAAGATAAACCTGGCAAGGAGTGTGCTGATGCT GCCTCCCCAGCTCTGTCCCTAGCTGAACTCAGGACAACGTGCAGCGAGAATGAGCTGGCTGCGGAGTTCA CCAACGCCATTCGTCGAGAAAAGAAGTTGAAGGCCAGAGTTCAAGAGCTGGTGAGTGCCTTGGAGAGACT CACCAAGAGCAGTGAAATCCGACATCAGCAATCTGCAGAGTTCGTGAATGATCTAAAGCGGGCCAACAGC AACCTGGTGGCTGCCTATGAGAAAGCAAAGAAAAAGCATCAAAACAAACTGAAGAAGTTAGAGTCGCAGA TGATGGCCATGGTGGAGAGACATGAGACCCAAGTGAGGATGCTCAAGCAAAGAATAGCTCTGCTAGAGGA GGAGAACTCCAGGCCACACACCAATGAAACTTCGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202890 protein sequence
Red=Cloning site Green=Tags(s) MNSGVAMKYGNDSSAELSELHSAALASLKGDIVELNKRLQQTERERDLLEKKLAKAQCEQSHLMREHEDV QERTTLRYEERITELHSVIAELNKKIDRLQGTTIREEDEYSELRSELSQSQHEVNEDSRSMDQDQTSVSI PENQSTMVTADMDNCSDLNSELQRVLTGLENVVCGRKKSSCSLSVAEVDRHIEQLTTASEHCDLAIKTVE EIEGVLGRDLYPNLAEERSRWEKELAGLREENESLTAMLCSKEEELNRTKATMNAIREERDRLRRRVREL QTRLQSVQATGPSSPGRLTSTNRPINPSTGELSTSSSSNDIPIAKIAERVKLSKTRSESSSSDRPVLGSE ISSIGVSSSVAEHLAHSLQDCSNIQEIFQTLYSHGSAISESKIREFEVETERLNSRIEHLKSQNDLLTIT LEECKSNAERMSMLVGKYESNATALRLALQYSEQCIEAYELLLALAESEQSLILGQFRAAGVGSSPGDQS GDENITQMLKRAHDCRKTAENAAKALLMKLDGSCGGAFAVAGCSVQPWESLSSNSHTSTTSSTASSCDTE FTKEDEQRLKDYIQQLKNDRAAVKLTMLELESIHIDPLSYDVKPRGDSQRLDLENAVLMQELMAMKEEMA ELKAQLYLLEKEKKALELKLSTREAQEQAYLVHIEHLKSEVEEQKEQRMRSLSSTSSGSKDKPGKECADA ASPALSLAELRTTCSENELAAEFTNAIRREKKLKARVQELVSALERLTKSSEIRHQQSAEFVNDLKRANS NLVAAYEKAKKKHQNKLKKLESQMMAMVERHETQVRMLKQRIALLEEENSRPHTNETSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002387 |
ORF Size | 2487 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002387.1, NM_002387.2, NP_002378.1 |
RefSeq Size | 8223 bp |
RefSeq ORF | 2490 bp |
Locus ID | 4163 |
Cytogenetics | 5q22.2 |
Protein Families | Druggable Genome |
MW | 93.1 kDa |
Gene Summary | 'This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC118657 | MCC (untagged)-Human mutated in colorectal cancers (MCC), transcript variant 2 |
USD 760.00 |
|
RG202890 | MCC (GFP-tagged) - Human mutated in colorectal cancers (MCC), transcript variant 2 |
USD 460.00 |
|
RC202890L3 | Lenti ORF clone of Human mutated in colorectal cancers (MCC), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC202890L4 | Lenti ORF clone of Human mutated in colorectal cancers (MCC), transcript variant 2, mGFP tagged |
USD 620.00 |
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