EXTL3 (NM_001440) Human Tagged ORF Clone

CAT#: RC202899

EXTL3 (Myc-DDK-tagged)-Human exostoses (multiple)-like 3 (EXTL3)

Product Images

Western blot validation of overexpression lysate (Cat# LY419936) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202899 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXTL3
• Synonyms: BOTV; EXTL1L; EXTR1; ISDNA; REGR; RPR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202899 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGGCTATACCATGCTGCGGAATGGGGGCGCGGGGAACGGAGGTCAGACCTGCATGCTGCGCTGGT
  CCAACCGCATCCGCCTCACGTGGCTCAGCTTCACGCTCTTTGTCATCCTGGTCTTCTTCCCGCTCATCGC
  CCACTATTACCTCACCACTCTGGATGAGGCTGATGAGGCAGGCAAGCGGATTTTTGGTCCCCGGGTGGGG
  AACGAGCTGTGCGAGGTGAAGCACGTGCTGGATCTGTGCCGCATCCGGGAGTCGGTGAGTGAAGAGCTCC
  TGCAGCTGGAGGCCAAGCGCCAAGAGCTGAACAGCGAGATCGCCAAGCTGAATCTGAAGATCGAAGCCTG
  TAAGAAGAGCATTGAGAACGCCAAGCAGGACCTGCTCCAGCTCAAGAATGTCATCAGCCAGACCGAGCAT
  TCCTACAAGGAGCTCATGGCCCAGAACCAGCCCAAGCTGTCCCTGCCCATCCGACTGCTCCCAGAGAAGG
  ACGATGCCGGCCTCCCTCCCCCGAAGGCCACTCGGGGCTGCCGGCTACACAACTGCTTTGATTATTCTCG
  TTGCCCTCTCACCTCTGGCTTCCCGGTCTACGTCTATGACAGTGACCAGTTTGTCTTTGGCAGCTACCTG
  GATCCCTTGGTCAAGCAGGCTTTTCAGGCGACAGCACGAGCTAACGTTTATGTTACAGAAAATGCAGACA
  TCGCCTGCCTTTACGTGATACTAGTGGGAGAGATGCAGGAGCCGGTGGTGCTGCGGCCTGCTGAGCTGGA
  GAAGCAGTTGTATTCCCTGCCACACTGGCGGACGGATGGACACAACCATGTCATCATCAATCTGTCACGT
  AAGTCAGATACACAGAACCTTCTCTATAACGTCAGTACTGGCCGTGCCATGGTGGCCCAGTCCACCTTCT
  ACACTGTCCAGTACAGACCTGGCTTTGACTTGGTCGTATCACCGCTGGTCCATGCCATGTCTGAGCCCAA
  CTTCATGGAAATCCCACCACAGGTGCCGGTGAAGCGGAAATATCTCTTCACCTTCCAGGGCGAGAAGATT
  GAGTCTCTGAGGTCTAGCCTTCAGGAGGCCCGCTCCTTCGAAGAGGAAATGGAGGGCGACCCTCCCGCCG
  ACTACGATGACCGGATCATTGCCACCCTGAAGGCGGTGCAGGACAGCAAGCTGGATCAGGTCCTGGTGGA
  ATTCACCTGCAAAAACCAGCCCAAACCCAGCCTGCCAACTGAGTGGGCACTGTGTGGAGAGCGGGAGGAC
  CGCTTGGAATTGCTGAAGCTCTCCACCTTCGCCCTCATCATTACCCCCGGGGACCCTCGCTTGGTTATTT
  CCTCTGGGTGTGCAACACGGCTCTTCGAAGCCCTGGAAGTCGGTGCCGTCCCGGTGGTGCTGGGGGAGCA
  GGTCCAGCTTCCCTACCAGGACATGCTGCAGTGGAACGAGGCGGCCCTGGTGGTGCCAAAGCCTCGTGTT
  ACCGAGGTTCATTTCCTGCTCAGAAGCCTCTCCGATAGTGACCTCCTGGCTATGAGGCGGCAAGGCCGCT
  TTCTCTGGGAGACTTACTTCTCCACTGCTGACAGTATTTTTAATACCGTGCTGGCTATGATTAGGACTCG
  CATCCAGATCCCAGCCGCTCCCATCCGGGAAGAGGCGGCAGCTGAGATCCCCCACCGTTCAGGCAAGGCG
  GCTGGAACTGACCCCAACATGGCTGACAACGGGGACCTGGACCTGGGGCCAGTGGAGACGGAGCCGCCCT
  ACGCCTCACCCAGATACCTCCGCAATTTCACTCTGACTGTCACTGACTTTTACCGCAGCTGGAACTGTGC
  TCCAGGGCCTTTCCATCTTTTCCCCCACACTCCCTTTGACCCTGTGTTGCCCTCAGAGGCCAAATTCTTG
  GGCTCAGGGACTGGCTTTCGGCCTATTGGTGGTGGAGCTGGGGGTTCTGGCAAGGAATTTCAGGCAGCGC
  TTGGAGGCAATGTTCCCCGAGAGCAGTTCACGGTGGTGATGTTGACTTATGAGCGGGAGGAAGTGCTTAT
  GAACTCTTTAGAGAGGCTGAATGGCCTCCCTTACCTGAACAAGGTCGTGGTGGTGTGGAATTCTCCCAAG
  CTGCCATCAGAGGACCTTCTGTGGCCTGACATTGGCGTCCCCATCATGGTGGTCCGTACTGAGAAGAACA
  GTTTGAACAACCGATTCTTACCCTGGAATGAAATTGAGACAGAGGCCATCCTGTCCATTGATGACGATGC
  TCACCTCCGCCATGACGAAATCATGTTTGGGTTCCGGGTGTGGAGAGAAGCTCGGGACCGCATCGTGGGC
  TTCCCTGGCCGTTACCACGCATGGGACATCCCCCATCAGTCCTGGCTCTACAACTCCAACTACTCCTGTG
  AGCTGTCCATGGTGCTGACAGGTGCTGCCTTCTTTCACAAGTATTATGCCTACCTGTATTCTTATGTGAT
  GCCCCAGGCCATCCGGGACATGGTGGATGAATACATCAACTGTGAGGACATTGCCATGAACTTCCTTGTC
  TCCCACATCACTCGGAAGCCCCCCATCAAGGTGACCTCACGGTGGACATTCCGATGCCCAGGATGCCCTC
  AGGCCCTGTCTCATGATGACTCCCACTTCCACGAGCGGCACAAGTGCATCAACTTCTTCGTGAAGGTGTA
  CGGCTACATGCCCCTCCTGTACACGCAGTTCAGGGTGGATTCTGTGCTCTTCAAGACACGCCTGCCCCAT
  GACAAGACCAAGTGCTTCAAGTTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202899 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTGYTMLRNGGAGNGGQTCMLRWSNRIRLTWLSFTLFVILVFFPLIAHYYLTTLDEADEAGKRIFGPRVG
  NELCEVKHVLDLCRIRESVSEELLQLEAKRQELNSEIAKLNLKIEACKKSIENAKQDLLQLKNVISQTEH
  SYKELMAQNQPKLSLPIRLLPEKDDAGLPPPKATRGCRLHNCFDYSRCPLTSGFPVYVYDSDQFVFGSYL
  DPLVKQAFQATARANVYVTENADIACLYVILVGEMQEPVVLRPAELEKQLYSLPHWRTDGHNHVIINLSR
  KSDTQNLLYNVSTGRAMVAQSTFYTVQYRPGFDLVVSPLVHAMSEPNFMEIPPQVPVKRKYLFTFQGEKI
  ESLRSSLQEARSFEEEMEGDPPADYDDRIIATLKAVQDSKLDQVLVEFTCKNQPKPSLPTEWALCGERED
  RLELLKLSTFALIITPGDPRLVISSGCATRLFEALEVGAVPVVLGEQVQLPYQDMLQWNEAALVVPKPRV
  TEVHFLLRSLSDSDLLAMRRQGRFLWETYFSTADSIFNTVLAMIRTRIQIPAAPIREEAAAEIPHRSGKA
  AGTDPNMADNGDLDLGPVETEPPYASPRYLRNFTLTVTDFYRSWNCAPGPFHLFPHTPFDPVLPSEAKFL
  GSGTGFRPIGGGAGGSGKEFQAALGGNVPREQFTVVMLTYEREEVLMNSLERLNGLPYLNKVVVVWNSPK
  LPSEDLLWPDIGVPIMVVRTEKNSLNNRFLPWNEIETEAILSIDDDAHLRHDEIMFGFRVWREARDRIVG
  FPGRYHAWDIPHQSWLYNSNYSCELSMVLTGAAFFHKYYAYLYSYVMPQAIRDMVDEYINCEDIAMNFLV
  SHITRKPPIKVTSRWTFRCPGCPQALSHDDSHFHERHKCINFFVKVYGYMPLLYTQFRVDSVLFKTRLPH
  DKTKCFKFI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001440
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001440.1, NM_001440.2, NM_001440.3, NP_001431.1
• RefSeq Size: 6351 bp
• RefSeq ORF: 2760 bp
• Locus ID: 2137
• Cytogenetics: 8p21.1
• Domains: Exostosin
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis, Metabolic pathways
• MW: 104.7 kDa
• Gene Summary: 'This gene encodes a single-pass membrane protein which functions as a glycosyltransferase. The encoded protein catalyzes the transfer of N-acetylglucosamine to glycosaminoglycan chains. This reaction is important in heparin and heparan sulfate synthesis. Alternative splicing results in the multiple transcript variants. [provided by RefSeq, Nov 2012]'