WFS1 (NM_006005) Human Tagged ORF Clone

CAT#: RC202901

WFS1 (Myc-DDK-tagged)-Human Wolfram syndrome 1 (wolframin) (WFS1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY429034) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227925 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified WFS1 protein (Cat# TP302901). The protein was produced from HEK293T cells transfected with WFS1 cDNA clone (Cat# RC202901) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WFS1
• Synonyms: CTRCT41; WFRS; WFS; WFSL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202901 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCAACACTGCTCCGCTGGGCCCCTCCTGCCCACAGCCCCCGCCAGCACCGCAGCCCCAGGCGC
  GTTCCCGACTCAATGCCACAGCCTCGTTGGAGCAGGAGAGGAGCGAAAGGCCCCGAGCACCCGGACCCCA
  GGCTGGCCCTGGCCCTGGTGTTAGAGACGCAGCGGCCCCCGCTGAACCCCAGGCCCAGCATACCAGGAGC
  CGGGAAAGAGCAGACGGCACCGGGCCTACAAAGGGAGACATGGAAATCCCCTTTGAAGAAGTCCTGGAGA
  GGGCCAAGGCCGGGGACCCCAAGGCACAGACTGAGGTGGGGAAGCACTACCTGCAGTTGGCCGGCGACAC
  GGATGAAGAACTCAACAGCTGCACCGCTGTGGACTGGCTGGTCCTCGCCGCGAAGCAGGGCCGTCGCGAG
  GCTGTGAAGCTGCTTCGCCGGTGCTTGGCGGACAGAAGAGGCATCACGTCCGAGAACGAACGGGAGGTGA
  GGCAGCTCTCCTCCGAGACCGACCTGGAGAGGGCCGTGCGCAAGGCAGCCCTGGTCATGTACTGGAAGCT
  CAACCCCAAGAAGAAGAAGCAGGTGGCCGTGGCGGAGCTGCTGGAGAATGTCGGCCAGGTCAACGAGCAC
  GATGGAGGGGCGCAGCCAGGCCCCGTGCCCAAGTCCCTGCAGAAGCAGAGGCGCATGCTGGAGCGCCTGG
  TCAGCAGCGAGTCCAAGAACTACATCGCGCTGGATGACTTTGTGGAGATCACTAAGAAGTACGCCAAGGG
  CGTCATCCCCAGCAGCCTGTTCCTGCAGGACGACGAAGATGATGACGAGCTGGCGGGGAAGAGCCCTGAG
  GACCTGCCACTGCGTCTGAAGGTGGTCAAGTACCCCCTGCACGCCATCATGGAGATCAAGGAGTACCTGA
  TTGACATGGCCTCCAGGGCAGGCATGCACTGGCTGTCCACCATCATCCCCACGCACCACATCAACGCGCT
  CATCTTCTTCTTCATCGTCAGCAACCTCACCATCGACTTCTTCGCCTTCTTCATCCCGCTGGTCATCTTC
  TACCTGTCCTTCATCTCCATGGTGATCTGCACCCTCAAGGTGTTCCAGGACAGCAAGGCCTGGGAGAACT
  TCCGCACCCTCACCGACCTGCTGCTGCGCTTCGAGCCCAACCTGGATGTGGAGCAGGCCGAGGTCAACTT
  CGGCTGGAACCACCTGGAGCCCTATGCCCATTTCCTGCTCTCTGTCTTCTTCGTCATCTTCTCCTTCCCC
  ATCGCCAGCAAGGACTGCATCCCCTGCTCGGAGCTGGCTGTCATCACCGGCTTCTTTACCGTGACCAGCT
  ACCTGAGCCTGAGCACCCATGCAGAGCCCTACACGCGCAGGGCCCTGGCCACCGAGGTCACCGCCGGCCT
  GCTATCGCTGCTGCCCTCCATGCCCTTGAATTGGCCCTACCTGAAGGTCCTTGGCCAGACCTTCATCACC
  GTGCCTGTCGGCCACCTGGTCGTCCTCAACGTCAGCGTCCCGTGCCTGCTCTATGTCTACCTGCTCTATC
  TCTTCTTCCGCATGGCACAGCTGAGGAATTTCAAGGGCACCTACTGCTACCTTGTGCCCTACCTGGTGTG
  CTTCATGTGGTGTGAGCTCTCCGTGGTCATCCTGCTGGAGTCCACCGGCCTGGGGCTGCTCCGCGCCTCC
  ATCGGCTACTTCCTCTTCCTCTTTGCCCTCCCCATCCTGGTGGCCGGCCTGGCCCTGGTGGGCGTGCTGC
  AGTTCGCCCGGTGGTTCACGTCTCTGGAGCTCACCAAGATCGCAGTCACCGTGGCGGTCTGTAGTGTGCC
  CCTGCTGTTGCGCTGGTGGACCAAGGCCAGCTTCTCTGTGGTGGGGATGGTGAAGTCCCTGACGCGGAGC
  TCCATGGTCAAGCTCATCCTGGTGTGGCTCACGGCCATCGTGCTGTTCTGCTGGTTCTATGTGTACCGCT
  CAGAGGGCATGAAGGTCTACAACTCCACACTGACCTGGCAGCAGTATGGTGCGCTGTGCGGGCCACGCGC
  CTGGAAGGAGACCAACATGGCGCGCACCCAGATCCTCTGCAGCCACCTGGAGGGCCACAGGGTCACGTGG
  ACCGGCCGCTTCAAGTACGTCCGCGTGACTGACATCGACAACAGCGCCGAGTCTGCCATCAACATGCTCC
  CGTTCTTCATCGGCGACTGGATGCGCTGCCTCTACGGCGAGGCCTACCCTGCCTGCAGCCCTGGCAACAC
  CTCCACGGCCGAGGAGGAGCTCTGTCGCCTTAAGCTGCTGGCCAAGCACCCCTGCCACATCAAGAAGTTC
  GACCGCTACAAGTTTGAGATTACCGTGGGCATGCCATTCAGCAGCGGCGCTGACGGCTCGCGCAGCCGCG
  AGGAGGACGACGTCACCAAGGACATCGTGCTGCGGGCCAGCAGCGAGTTCAAGAGCGTGCTGCTCAGCCT
  GCGCCAGGGCAGCCTCATCGAGTTCAGCACCATCCTGGAGGGCCGCCTGGGCAGCAAGTGGCCTGTCTTC
  GAGCTCAAGGCCATCAGCTGCCTCAACTGCATGGCCCAGCTCTCACCCACCAGGCGGCACGTGAAGATCG
  AGCACGACTGGCGCAGCACCGTGCATGGCGCCGTGAAGTTCGCCTTCGACTTCTTTTTCTTCCCATTCCT
  GTCGGCGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202901 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSNTAPLGPSCPQPPPAPQPQARSRLNATASLEQERSERPRAPGPQAGPGPGVRDAAAPAEPQAQHTRS
  RERADGTGPTKGDMEIPFEEVLERAKAGDPKAQTEVGKHYLQLAGDTDEELNSCTAVDWLVLAAKQGRRE
  AVKLLRRCLADRRGITSENEREVRQLSSETDLERAVRKAALVMYWKLNPKKKKQVAVAELLENVGQVNEH
  DGGAQPGPVPKSLQKQRRMLERLVSSESKNYIALDDFVEITKKYAKGVIPSSLFLQDDEDDDELAGKSPE
  DLPLRLKVVKYPLHAIMEIKEYLIDMASRAGMHWLSTIIPTHHINALIFFFIVSNLTIDFFAFFIPLVIF
  YLSFISMVICTLKVFQDSKAWENFRTLTDLLLRFEPNLDVEQAEVNFGWNHLEPYAHFLLSVFFVIFSFP
  IASKDCIPCSELAVITGFFTVTSYLSLSTHAEPYTRRALATEVTAGLLSLLPSMPLNWPYLKVLGQTFIT
  VPVGHLVVLNVSVPCLLYVYLLYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILLESTGLGLLRAS
  IGYFLFLFALPILVAGLALVGVLQFARWFTSLELTKIAVTVAVCSVPLLLRWWTKASFSVVGMVKSLTRS
  SMVKLILVWLTAIVLFCWFYVYRSEGMKVYNSTLTWQQYGALCGPRAWKETNMARTQILCSHLEGHRVTW
  TGRFKYVRVTDIDNSAESAINMLPFFIGDWMRCLYGEAYPACSPGNTSTAEEELCRLKLLAKHPCHIKKF
  DRYKFEITVGMPFSSGADGSRSREEDDVTKDIVLRASSEFKSVLLSLRQGSLIEFSTILEGRLGSKWPVF
  ELKAISCLNCMAQLSPTRRHVKIEHDWRSTVHGAVKFAFDFFFFPFLSAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006005
• ORF Size: 2670 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006005.1, NM_006005.2, NM_006005.3, NP_005996.1
• RefSeq Size: 3640 bp
• RefSeq ORF: 2673 bp
• Locus ID: 7466
• Cytogenetics: 4p16.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 100.3 kDa
• Gene Summary: 'This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]'