WFS1 (NM_006005) Human Untagged Clone

CAT#: SC319529

WFS1 (untagged)-Human Wolfram syndrome 1 (wolframin) (WFS1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WFS1
• Synonyms: CTRCT41; WFRS; WFS; WFSL
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006005.2 CTCCCGTTTGCGCCGCGTTCAGCTGCTCCCGAACAACTTTTCTGCCGGCCCAGAGGCCCC
  AGGGCGTCGCAGCGCCGCGTGCGGCCCACTCACGGGCCGGATGGACTCCAACACTGCTCC
  GCTGGGCCCCTCCTGCCCACAGCCCCCGCCAGCACCGCAGCCCCAGGCGCGTTCCCGACT
  CAATGCCACAGCCTCGTTGGAGCAGGAGAGGAGCGAAAGGCCCCGAGCACCCGGACCCCA
  GGCTGGCCCTGGCCCTGGTGTTAGAGACGCAGCGGCCCCCGCTGAACCCCAGGCCCAGCA
  TACCAGGAGCCGGGAAAGAGCAGACGGCACCGGGCCTACAAAGGGAGACATGGAAATCCC
  CTTTGAAGAAGTCCTGGAGAGGGCCAAGGCCGGGGACCCCAAGGCACAGACTGAGGTGGG
  GAAGCACTACCTGCAGTTGGCCGGCGACACGGATGAAGAACTCAACAGCTGCACCGCTGT
  GGACTGGCTGGTCCTCGCCGCGAAGCAGGGCCGTCGCGAGGCTGTGAAGCTGCTTCGCCG
  GTGCTTGGCGGACAGAAGAGGCATCACGTCCGAGAACGAACGGGAGGTGAGGCAGCTCTC
  CTCCGAGACCGACCTGGAGAGGGCCGTGCGCAAGGCAGCCCTGGTCATGTACTGGAAGCT
  CAACCCCAAGAAGAAGAAGCAGGTGGCCGTGGCGGAGCTGCTGGAGAATGTCGGCCAGGT
  CAACGAGCACGATGGAGGGGCGCAGCCAGGCCCCGTGCCCAAGTCCCTGCAGAAGCAGAG
  GCGCATGCTGGAGCGCCTGGTCAGCAGCGAGTCCAAGAACTACATCGCGCTGGATGACTT
  TGTGGAGATCACTAAGAAGTACGCCAAGGGCGTCATCCCCAGCAGCCTGTTCCTGCAGGA
  CGACGAAGATGATGACGAGCTGGCGGGGAAGAGCCCTGAGGACCTGCCACTGCGTCTGAA
  GGTGGTCAAGTACCCCCTGCACGCCATCATGGAGATCAAGGAGTACCTGATTGACATGGC
  CTCCAGGGCAGGCATGCACTGGCTGTCCACCATCATCCCCACGCACCACATCAACGCGCT
  CATCTTCTTCTTCATCGTCAGCAACCTCACCATCGACTTCTTCGCCTTCTTCATCCCGCT
  GGTCATCTTCTACCTGTCCTTCATCTCCATGGTGATCTGCACCCTCAAGGTGTTCCAGGA
  CAGCAAGGCCTGGGAGAACTTCCGCACCCTCACCGACCTGCTGCTGCGCTTCGAGCCCAA
  CCTGGATGTGGAGCAGGCCGAGGTCAACTTCGGCTGGAACCACCTGGAGCCCTATGCCCA
  TTTCCTGCTCTCTGTCTTCTTCGTCATCTTCTCCTTCCCCATCGCCAGCAAGGACTGCAT
  CCCCTGCTCGGAGCTGGCTGTCATCACCGGCTTCTTTACCGTGACCAGCTACCTGAGCCT
  GAGCACCCATGCAGAGCCCTACACGCGCAGGGCCCTGGCCACCGAGGTCACCGCCGGCCT
  GCTATCGCTGCTGCCCTCCATGCCCTTGAATTGGCCCTACCTGAAGGTCCTTGGCCAGAC
  CTTCATCACCGTGCCTGTCGGCCACCTGGTCGTCCTCAACGTCAGCGTCCCGTGCCTGCT
  CTATGTCTACCTGCTCTATCTCTTCTTCCGCATGGCACAGCTGAGGAATTTCAAGGGCAC
  CTACTGCTACCTTGTGCCCTACCTGGTGTGCTTCATGTGGTGTGAGCTCTCCGTGGTCAT
  CCTGCTGGAGTCCACCGGCCTGGGGCTGCTCCGCGCCTCCATCGGCTACTTCCTCTTCCT
  CTTTGCCCTCCCCATCCTGGTGGCCGGCCTGGCCCTGGTGGGCGTGCTGCAGTTCGCCCG
  GTGGTTCACGTCTCTGGAGCTCACCAAGATCGCAGTCACCGTGGCGGTCTGTAGTGTGCC
  CCTGCTGTTGCGCTGGTGGACCAAGGCCAGCTTCTCTGTGGTGGGGATGGTGAAGTCCCT
  GACGCGGAGCTCCATGGTCAAGCTCATCCTGGTGTGGCTCACGGCCATCGTGCTGTTCTG
  CTGGTTCTATGTGTACCGCTCAGAGGGCATGAAGGTCTACAACTCCACACTGACCTGGCA
  GCAGTATGGTGCGCTGTGCGGGCCACGCGCCTGGAAGGAGACCAACATGGCGCGCACCCA
  GATCCTCTGCAGCCACCTGGAGGGCCACAGGGTCACGTGGACCGGCCGCTTCAAGTACGT
  CCGCGTGACTGACATCGACAACAGCGCCGAGTCTGCCATCAACATGCTCCCGTTCTTCAT
  CGGCGACTGGATGCGCTGCCTCTACGGCGAGGCCTACCCTGCCTGCAGCCCTGGCAACAC
  CTCCACGGCCGAGGAGGAGCTCTGTCGCCTTAAGCTGCTGGCCAAGCACCCCTGCCACAT
  CAAGAAGTTCGACCGCTACAAGTTTGAGATTACCGTGGGCATGCCATTCAGCAGCGGCGC
  TGACGGCTCGCGCAGCCGCGAGGAGGACGACGTCACCAAGGACATCGTGCTGCGGGCCAG
  CAGCGAGTTCAAGAGCGTGCTGCTCAGCCTGCGCCAGGGCAGCCTCATCGAGTTCAGCAC
  CATCCTGGAGGGCCGCCTGGGCAGCAAGTGGCCTGTCTTCGAGCTCAAGGCCATCAGCTG
  CCTCAACTGCATGGCCCAGCTCTCACCCACCAGGCGGCACGTGAAGATCGAGCACGACTG
  GCGCAGCACCGTGCATGGCGCCGTGAAGTTCGCCTTCGACTTCTTTTTCTTCCCATTCCT
  GTCGGCGGCCTGAGGATGGTCCGCCACGAGGAGCTTCCAGTGCATGTTGCCATGAGGCCT
  TTCCCCAGTGTGGCCCCAGCCCGACAGGCATGCACCAGTGCCGCCTGTGCCCACGTGTGC
  AGACTGTGGCTGCAGAGACCTTGCGACCATGTGTAGATTGCGTGGACCCCGACAAAGGGA
  AGGCTGCTGCTGAATACCAAGTGTGTTGGGAATTGCATGCCATCTCCACCCTGAGCCTGA
  CCTTTCTGAGTGACATGGGTGTGCCAGGCTAGACTAGGAGGTTCCGGTGTCTGGAAAAGC
  ACTTTACAGATGAGATTCCCTCTCCTCCCCCACCTTCAAGCACCCTGTTCCCTCTTTCTT
  TCTTTTGTGTTGGATTTGTTTAAAAACCAAATAAGCATCTGTGTAACCTCCAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006005
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006005.2, NP_005996.1
• RefSeq Size: 3640 bp
• RefSeq ORF: 2673 bp
• Locus ID: 7466
• Cytogenetics: 4p16.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (1) is the longer transcript. Variants 1 and 2 encode the same protein.